##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbrPI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BsrBI BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI HincII
HindII HindIII HpaI KpnI KspI
MaeII MamI MfeI MflI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI SspBI SspI
SwaI Tsp509I Tth111I XhoII XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 418 TCCGGA T^CCGGA
AflIII 1391 ACATGT A^C[GA][CT]GT
Alw44I 1054 GTGCAC G^TGCAC
AlwI 421 GGATC GGATC(4/5)
AocI 1709 CCTCAGG CC^T[ACGT]AGG
ApaLI 1054 GTGCAC G^TGCAC
BalI 797 TGGCCA TGG^CCA
BbsI 1715 GAAGAC GAAGAC(2/6)
BbvCI 885 GCTGAGG CCTCAGC(-5/-2)
BpuAI 1715 GAAGAC GAAGAC(2/6)
BsaWI 418 TCCGGA [AT]^CCGG[AT]
BseAI 418 TCCGGA T^CCGGA
BspEI 418 TCCGGA T^CCGGA
BspLU11I 1391 ACATGT A^CATGT
BstAPI 1698 GCACGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1709 CCTCAGG CC^T[ACGT]AGG
Eco81I 1709 CCTCAGG CC^T[ACGT]AGG
FseI 1739 GGCCGGCC GGCCGG^CC
HgaI 1531 GCGTC GACGC(5/10)
MaeIII 974 GTGAC ^GT[ACGT]AC
MluNI 797 TGGCCA TGG^CCA
MroI 418 TCCGGA T^CCGGA
MscI 797 TGGCCA TGG^CCA
NspI 1391 ACATGT [GA]CATG^[CT]
SgrAI 59 CACCGGCG C[GA]^CCGG[CT]G
StuI 1273 AGGCCT AGG^CCT
Tsp45I 974 GTGAC ^GT[GC]AC
XbaI 1655 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
ApaI 1346 GGGCCC GGGCC^C
1421 GGGCCC
AviII 165 TGCGCA TGC^GCA
929 TGCGCA
BbeI 88 GGCGCC GGCGC^C
196 GGCGCC
BfaI 1351 CTAG C^TAG
1656 CTAG
BglI 1357 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1414 GCCAAGCGGGC
BssHII 64 GCGCGC G^CGCGC
527 GCGCGC
DpnII 422 GATC ^GATC
1458 GATC
EcoNI 1605 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1844 CCTTGGCAAGG
FspI 165 TGCGCA TGC^GCA
929 TGCGCA
KasI 88 GGCGCC G^GCGCC
196 GGCGCC
MaeI 1351 CTAG C^TAG
1656 CTAG
MboI 422 GATC ^GATC
1458 GATC
MseI 692 TTAA T^TAA
1858 TTAA
NarI 88 GGCGCC GG^CGCC
196 GGCGCC
NcoI 572 CCATGG C^CATGG
714 CCATGG
NdeII 422 GATC ^GATC
1458 GATC
PaeR7I 19 CTCGAG C^TCGAG
1169 CTCGAG
PflMI 1152 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1188 CCAGTGTGTGG
Sau3AI 422 GATC ^GATC
1458 GATC
SbfI 82 CCTGCAGG CCTGCA^GG
774 CCTGCAGG
SfoI 88 GGCGCC GGC^GCC
196 GGCGCC
SmaI 43 CCCGGG CCC^GGG
333 CCCGGG
SmlI 19 CTCGAG C^T[CT][GA]AG
1169 CTCGAG
Sse8387I 82 CCTGCAGG CCTGCA^GG
774 CCTGCAGG
TfiI 208 GATTC G^A[AT]TC
457 GAATC
Tru9I 692 TTAA T^TAA
1858 TTAA
Van91I 1152 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1188 CCAGTGTGTGG
XcmI 1148 CCATCCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1665 CCACAAGCACTATGG
XhoI 19 CTCGAG C^TCGAG
1169 CTCGAG
XmaCI 43 CCCGGG C^CCGGG
333 CCCGGG
XmaI 43 CCCGGG C^CCGGG
333 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NgoMIV NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI TspRI TthHB8I