##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
Alw44I AlwNI ApaI ApaLI AscI
AspI AviII BalI BfrI BglII
BsaBI BseAI BsgI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BsrBI
BstAPI BstBI ClaI CpoI EagI
EclXI Eco52I EcoRV FseI FspI
MamI MluI MluNI MroI MscI
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SbfI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
Van91I XbaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 1839 GACGTC GACGT^C
Acc65I 244 GGTACC G^GTACC
AhdI 900 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 943 CCTGAGG CC^T[ACGT]AGG
Aor51HI 207 AGCGCT AGC^GCT
AseI 3585 ATTAAT AT^TAAT
AsnI 3585 ATTAAT AT^TAAT
Asp718I 244 GGTACC G^GTACC
AspEI 900 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3623 CCTAGG C^CTAGG
BamHI 195 GGATCC G^GATCC
BbrPI 736 CACGTG CAC^GTG
BbsI 1213 GTCTTC GAAGAC(2/6)
BbvCI 565 GCTGAGG CCTCAGC(-5/-2)
BcgI 386 CGACGCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1536 TGATCA T^GATCA
BlnI 3623 CCTAGG C^CTAGG
BpuAI 1213 GTCTTC GAAGAC(2/6)
BsaAI 736 CACGTG [CT]AC^GT[GA]
BsaI 898 GAGACC GGTCTC(1/5)
BsiEI 529 CGGTCG CG[GA][CT]^CG
BsrDI 2706 CATTGC GCAATG(2/0)
BsrFI 378 GCCGGC [GA]^CCGG[CT]
BssHII 521 GCGCGC G^CGCGC
BssSI 249 CACGAG CACGAG(-5/-1)
Bst1107I 3775 GTATAC GTA^TAC
BstXI 3273 CCATGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3775 GTATAC GTA^TAC
Bsu36I 943 CCTGAGG CC^T[ACGT]AGG
Cfr10I 378 GCCGGC [GA]^CCGG[CT]
DraIII 11 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 357 GACAACAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 821 TGGCCG [CT]^GGCC[GA]
Eam1105I 900 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 207 AGCGCT AGC^GCT
Eco81I 943 CCTGAGG CC^T[ACGT]AGG
EcoNI 750 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1536 TGATCA T^GATCA
HpaI 3884 GTTAAC GTT^AAC
KpnI 244 GGTACC GGTAC^C
MfeI 3317 CAATTG C^AATTG
MunI 3317 CAATTG C^AATTG
NaeI 378 GCCGGC GCC^GGC
NdeI 2209 CATATG CA^TATG
NgoMIV 378 GCCGGC G^CCGGC
PaeR7I 201 CTCGAG C^TCGAG
PmaCI 736 CACGTG CAC^GTG
PmlI 736 CACGTG CAC^GTG
PshBI 3585 ATTAAT AT^TAAT
PstI 1805 CTGCAG CTGCA^G
SalI 531 GTCGAC G^TCGAC
SapI 973 GAAGAGC GCTCTTC(1/4)
SfiI 282 GGCCCGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 232 CCCGGG CCC^GGG
StuI 306 AGGCCT AGG^CCT
TfiI 1935 GAATC G^A[AT]TC
XhoI 201 CTCGAG C^TCGAG
XmaCI 232 CCCGGG C^CCGGG
XmaI 232 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BbeI 254 GGCGCC GGCGC^C
460 GGCGCC
BciVI 3801 GGATAC GTATCC(6/5)
723 GTATCC
BfaI 3121 CTAG C^TAG
3624 CTAG
BglI 283 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
700 GCCTCCTGGGC
BlpI 1940 GCTCAGC GC^T[ACGT]AGC
2444 GCTCAGC
Bpu1102I 1940 GCTCAGC GC^T[ACGT]AGC
2444 GCTCAGC
BsaWI 527 TCCGGT [AT]^CCGG[AT]
2714 ACCGGA
CelII 1940 GCTCAGC GC^T[ACGT]AGC
2444 GCTCAGC
DraI 2239 TTTAAA TTT^AAA
3902 TTTAAA
EcoT22I 2218 ATGCAT ATGCA^T
2890 ATGCAT
HindIII 2261 AAGCTT A^AGCTT
3171 AAGCTT
KasI 254 GGCGCC G^GCGCC
460 GGCGCC
MaeI 3121 CTAG C^TAG
3624 CTAG
NarI 254 GGCGCC GG^CGCC
460 GGCGCC
NsiI 2218 ATGCAT ATGCA^T
2890 ATGCAT
NspI 997 GCATGT [GA]CATG^[CT]
1347 ACATGC
ScaI 1230 AGTACT AGT^ACT
3406 AGTACT
SfoI 254 GGCGCC GGC^GCC
460 GGCGCC
SmlI 201 CTCGAG C^T[CT][GA]AG
3265 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I KspI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI