##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccI AclI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII BciVI
BclI BsaBI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI Eam1105I Eco47III
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HpaI KpnI MamI MfeI
MluI MunI NheI NotI NsiI
NspV PacI PaeR7I PflFI PmeI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SphI Sse8387I SspBI
SspI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 3542 TCCGGA T^CCGGA
AflII 3192 CTTAAG C^TTAAG
AflIII 1161 ACACGT A^C[GA][CT]GT
AgeI 3622 ACCGGT A^CCGGT
Asp700I 1285 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2251 CCTAGG C^CTAGG
BamHI 1690 GGATCC G^GATCC
BbeI 3103 GGCGCC GGCGC^C
BbrPI 2561 CACGTG CAC^GTG
BcgI 381 GCATCATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3192 CTTAAG C^TTAAG
BglI 3078 GCCAGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5074 AGATCT A^GATCT
BlnI 2251 CCTAGG C^CTAGG
BsaAI 2561 CACGTG [CT]AC^GT[GA]
BseAI 3542 TCCGGA T^CCGGA
BsiEI 913 CGGCCG CG[GA][CT]^CG
BspEI 3542 TCCGGA T^CCGGA
BspHI 577 TCATGA T^CATGA
BsrDI 2424 GCAATG GCAATG(2/0)
DraIII 3153 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 913 CGGCCG C^GGCCG
EclXI 913 CGGCCG C^GGCCG
Eco52I 913 CGGCCG C^GGCCG
EcoRV 2702 GATATC GAT^ATC
HindIII 5141 AAGCTT A^AGCTT
KasI 3103 GGCGCC G^GCGCC
KspI 910 CCGCGG CCGC^GG
MroI 3542 TCCGGA T^CCGGA
NaeI 816 GCCGGC GCC^GGC
NarI 3103 GGCGCC GG^CGCC
NdeI 2351 CATATG CA^TATG
NgoMIV 816 GCCGGC G^CCGGC
NruI 1124 TCGCGA TCG^CGA
NspI 5031 ACATGC [GA]CATG^[CT]
PinAI 3622 ACCGGT A^CCGGT
PmaCI 2561 CACGTG CAC^GTG
PmlI 2561 CACGTG CAC^GTG
PstI 3940 CTGCAG CTGCA^G
RcaI 577 TCATGA T^CATGA
SacII 910 CCGCGG CCGC^GG
SfoI 3103 GGCGCC GGC^GCC
SpeI 2540 ACTAGT A^CTAGT
XbaI 2290 TCTAGA T^CTAGA
XmnI 1285 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 9 enzymes) #
###################################
AatII 1749 GACGTC GACGT^C
3736 GACGTC
AcsI 3218 GAATTT [GA]^AATT[CT]
4508 AAATTC
ApoI 3218 GAATTT [GA]^AATT[CT]
4508 AAATTC
BbsI 2676 GAAGAC GAAGAC(2/6)
3827 GAAGAC
BpuAI 2676 GAAGAC GAAGAC(2/6)
3827 GAAGAC
HincII 1020 GTCAAC GT[CT]^[GA]AC
4882 GTTGAC
HindII 1020 GTCAAC GT[CT]^[GA]AC
4882 GTTGAC
MslI 2561 CACGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4901 CATGAGAATG
ScaI 4796 AGTACT AGT^ACT
4861 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PshAI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI