##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccIII AclI AflII AgeI Aor51HI
AscI BbeI BbrPI BclI BfrI
BsaAI BsaBI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DraIII
Eco47III EcoT22I FbaI FseI KasI
KspI MamI MluI MroI NarI
NdeI NheI NruI NsiI NspV
PacI PinAI PmaCI PmlI Psp1406I
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 3315 GACGTC GACGT^C
Acc65I 4224 GGTACC G^GTACC
AccI 2281 GTCTAC GT^[AC][GT]AC
AflIII 1370 ACATGT A^C[GA][CT]GT
Alw44I 2580 GTGCAC G^TGCAC
ApaLI 2580 GTGCAC G^TGCAC
AseI 5912 ATTAAT AT^TAAT
AsnI 5912 ATTAAT AT^TAAT
Asp718I 4224 GGTACC G^GTACC
AviII 3565 TGCGCA TGC^GCA
BamHI 3288 GGATCC G^GATCC
BcgI 5929 CGAATCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI 145 TCCGGT [AT]^CCGG[AT]
BspHI 927 TCATGA T^CATGA
BspLU11I 1370 ACATGT A^CATGT
BssSI 445 CTCGTG CACGAG(-5/-1)
BstEII 2708 GGTCACC G^GT[ACGT]ACC
BstPI 2708 GGTCACC G^GT[ACGT]ACC
BstXI 3391 CCACCGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I 2708 GGTCACC G^GT[ACGT]ACC
EcoRI 2725 GAATTC G^AATTC
EcoRV 2254 GATATC GAT^ATC
FspI 3565 TGCGCA TGC^GCA
HaeII 333 AGCGCC [GA]GCGC^[CT]
HindIII 4616 AAGCTT A^AGCTT
HpaI 4285 GTTAAC GTT^AAC
KpnI 4224 GGTACC GGTAC^C
MfeI 1528 CAATTG C^AATTG
MunI 1528 CAATTG C^AATTG
NotI 1 GCGGCCGC GC^GGCCGC
PaeR7I 5193 CTCGAG C^TCGAG
PmeI 2586 GTTTAAAC GTTT^AAAC
PpuMI 653 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 5912 ATTAAT AT^TAAT
PvuI 297 CGATCG CGAT^CG
RcaI 927 TCATGA T^CATGA
SexAI 318 ACCTGGT A^CC[AT]GGT
SpeI 1068 ACTAGT A^CTAGT
XhoI 5193 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AhdI 315 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4309 GACAGAGAGTC
AlwNI 5278 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
5675 CAGCTACTG
Asp700I 980 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2120 GAATTGATTC
AspEI 315 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4309 GACAGAGAGTC
AspI 5200 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5248 GACCAAGTC
BalI 1945 TGGCCA TGG^CCA
2210 TGGCCA
BciVI 1116 GGATAC GTATCC(6/5)
4486 GTATCC
BglII 3879 AGATCT A^GATCT
5827 AGATCT
BsmI 4568 GCATTC GAATGC(1/-1)
4712 GCATTC
BssHII 3279 GCGCGC G^CGCGC
3407 GCGCGC
DraI 2239 TTTAAA TTT^AAA
2587 TTTAAA
DrdI 2140 GACTCTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5090 GACACCCACGTC
Eam1105I 315 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4309 GACAGAGAGTC
MluNI 1945 TGGCCA TGG^CCA
2210 TGGCCA
MscI 1945 TGGCCA TGG^CCA
2210 TGGCCA
MslI 2804 CACATTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5233 CATCATGGTG
NcoI 171 CCATGG C^CATGG
4904 CCATGG
PflFI 5200 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5248 GACCAAGTC
PflMI 1948 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2202 CCAACACCTGG
PshAI 5036 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5309 GACAATGGTC
SspI 1013 AATATT AAT^ATT
1356 AATATT
Tth111I 5200 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5248 GACCAAGTC
Van91I 1948 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2202 CCAACACCTGG
XmnI 980 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2120 GAATTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AocI ApaI ApoI
AspHI AvaI AvaII AvrII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI