##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AocI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII BclI BfrI BglII BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrBI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
Eam1105I Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HpaI KpnI
MfeI MluI MunI NcoI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Alw44I 5 GTGCAC G^TGCAC
Aor51HI 2073 AGCGCT AGC^GCT
ApaLI 5 GTGCAC G^TGCAC
AspI 217 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2443 CCTAGG C^CTAGG
BbeI 2341 GGCGCC GGCGC^C
BbrPI 2232 CACGTG CAC^GTG
BcgI 2022 CGAGGAACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 585 GGATAC GTATCC(6/5)
BlnI 2443 CCTAGG C^CTAGG
BpmI 1263 CTGGAG CTGGAG(16/14)
BsiEI 449 CGGCCG CG[GA][CT]^CG
BsmBI 445 GAGACG CGTCTC(1/5)
BspHI 1688 TCATGA T^CATGA
BsrDI 413 CATTGC GCAATG(2/0)
BssHII 1455 GCGCGC G^CGCGC
BssSI 1101 CACGAG CACGAG(-5/-1)
DraIII 553 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1247 CCGCGG C^C[GA][CT]GG
EagI 449 CGGCCG C^GGCCG
EclXI 449 CGGCCG C^GGCCG
Eco47III 2073 AGCGCT AGC^GCT
Eco52I 449 CGGCCG C^GGCCG
HincII 2372 GTTGAC GT[CT]^[GA]AC
HindII 2372 GTTGAC GT[CT]^[GA]AC
KasI 2341 GGCGCC G^GCGCC
KspI 1247 CCGCGG CCGC^GG
NaeI 870 GCCGGC GCC^GGC
NarI 2341 GGCGCC GG^CGCC
NgoMIV 870 GCCGGC G^CCGGC
NheI 547 GCTAGC G^CTAGC
PflFI 217 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2232 CACGTG CAC^GTG
PmlI 2232 CACGTG CAC^GTG
RcaI 1688 TCATGA T^CATGA
SacII 1247 CCGCGG CCGC^GG
SapI 2498 GCTCTTC GCTCTTC(1/4)
SfiI 1089 GGCCAAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2341 GGCGCC GGC^GCC
SmaI 1952 CCCGGG CCC^GGG
SmlI 1344 CTCAAG C^T[CT][GA]AG
SphI 2475 GCATGC GCATG^C
StuI 342 AGGCCT AGG^CCT
Tth111I 217 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1952 CCCGGG C^CCGGG
XmaI 1952 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 426 TCCGGA T^CCGGA
2315 TCCGGA
AcsI 262 GAATTC [GA]^AATT[CT]
1867 GAATTC
ApoI 262 GAATTC [GA]^AATT[CT]
1867 GAATTC
BbsI 196 GAAGAC GAAGAC(2/6)
853 GTCTTC
BpuAI 196 GAAGAC GAAGAC(2/6)
853 GTCTTC
BsaAI 1351 CACGTA [CT]AC^GT[GA]
2232 CACGTG
BsaBI 1443 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2055 GATGAATATC
BsaWI 426 TCCGGA [AT]^CCGG[AT]
2315 TCCGGA
BseAI 426 TCCGGA T^CCGGA
2315 TCCGGA
BsgI 1599 CTGCAC GTGCAG(16/14)
1124 GTGCAG
BspEI 426 TCCGGA T^CCGGA
2315 TCCGGA
BsrFI 818 ACCGGC [GA]^CCGG[CT]
870 GCCGGC
Cfr10I 818 ACCGGC [GA]^CCGG[CT]
870 GCCGGC
EarI 2499 CTCTTC CTCTTC(1/4)
232 GAAGAG
EcoRI 262 GAATTC G^AATTC
1867 GAATTC
HindIII 162 AAGCTT A^AGCTT
330 AAGCTT
Ksp632I 2499 CTCTTC CTCTTC(1/4)
232 GAAGAG
MamI 1443 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2055 GATGAATATC
MroI 426 TCCGGA T^CCGGA
2315 TCCGGA
MseI 1427 TTAA T^TAA
2561 TTAA
MslI 1201 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1683 CATCTTCATG
PflMI 396 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
552 CCACCGGGTGG
PstI 1387 CTGCAG CTGCA^G
2381 CTGCAG
SacI 123 GAGCTC GAGCT^C
2113 GAGCTC
Tru9I 1427 TTAA T^TAA
2561 TTAA
Tsp45I 673 GTCAC ^GT[GC]AC
1378 GTGAC
Van91I 396 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
552 CCACCGGGTGG
XcmI 969 CCAGACGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1085 CCAAGGCCAAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI Bpu1102I BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DrdI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp509I TspRI TthHB8I
XhoII