##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI Aor51HI AscI Asp718I AspI
AviII BcgI BciVI BclI BglII
BseAI BsiWI BsmBI BsmI BspDI
BspEI BssHII Bst1107I BstAPI BstZ17I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MfeI MluI MroI MunI
NaeI NgoMIV NheI NotI NruI
NsiI PacI PflFI PinAI PmeI
PshAI Psp1406I RsrII SalI SapI
SbfI SfiI SgrAI SnaBI SpeI
SphI Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AflII 3674 CTTAAG C^TTAAG
AhdI 1992 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 510 GTGCAC G^TGCAC
ApaLI 510 GTGCAC G^TGCAC
AseI 3476 ATTAAT AT^TAAT
AsnI 3476 ATTAAT AT^TAAT
AspEI 1992 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3013 CCTAGG C^CTAGG
BamHI 1950 GGATCC G^GATCC
BbrPI 168 CACGTG CAC^GTG
BfrI 3674 CTTAAG C^TTAAG
BlnI 3013 CCTAGG C^CTAGG
BsaAI 168 CACGTG [CT]AC^GT[GA]
BsaBI 932 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2880 GGTCTC GGTCTC(1/5)
BsaWI 3397 ACCGGA [AT]^CCGG[AT]
BsiEI 38 CGATCG CG[GA][CT]^CG
BspLU11I 838 ACATGT A^CATGT
BsrDI 3760 CATTGC GCAATG(2/0)
BsrFI 1732 ACCGGC [GA]^CCGG[CT]
BstBI 2219 TTCGAA TT^CGAA
BstEII 1883 GGTTACC G^GT[ACGT]ACC
BstPI 1883 GGTTACC G^GT[ACGT]ACC
Cfr10I 1732 ACCGGC [GA]^CCGG[CT]
DraIII 115 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1992 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1883 GGTTACC G^GT[ACGT]ACC
EcoRI 3788 GAATTC G^AATTC
KspI 1387 CCGCGG CCGC^GG
MamI 932 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 664 CATATG CA^TATG
NspV 2219 TTCGAA TT^CGAA
PflMI 3072 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 168 CACGTG CAC^GTG
PmlI 168 CACGTG CAC^GTG
PshBI 3476 ATTAAT AT^TAAT
PvuI 38 CGATCG CGAT^CG
SacII 1387 CCGCGG CCGC^GG
ScaI 806 AGTACT AGT^ACT
SfuI 2219 TTCGAA TT^CGAA
SspI 531 AATATT AAT^ATT
StuI 2686 AGGCCT AGG^CCT
Van91I 3072 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 643 ACACGT A^C[GA][CT]GT
838 ACATGT
Asp700I 308 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1192 GAACAAGTTC
BalI 1718 TGGCCA TGG^CCA
3989 TGGCCA
BbvCI 4379 CCTCAGC CCTCAGC(-5/-2)
188 GCTGAGG
BspMI 2957 ACCTGC ACCTGC(4/8)
3038 ACCTGC
BssSI 1487 CACGAG CACGAG(-5/-1)
444 CTCGTG
HgaI 392 GACGC GACGC(5/10)
4555 GCGTC
HincII 842 GTCAAC GT[CT]^[GA]AC
4689 GTTGAC
HindII 842 GTCAAC GT[CT]^[GA]AC
4689 GTTGAC
MluNI 1718 TGGCCA TGG^CCA
3989 TGGCCA
MscI 1718 TGGCCA TGG^CCA
3989 TGGCCA
MslI 2183 CACGGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4835 CACTGGCGTG
NspI 838 ACATGT [GA]CATG^[CT]
4507 GCATGT
PvuII 285 CAGCTG CAG^CTG
2237 CAGCTG
SacI 2336 GAGCTC GAGCT^C
4292 GAGCTC
SexAI 400 ACCTGGT A^CC[AT]GGT
4785 ACCTGGT
SmaI 29 CCCGGG CCC^GGG
1556 CCCGGG
XbaI 3467 TCTAGA T^CTAGA
3982 TCTAGA
XmaCI 29 CCCGGG C^CCGGG
1556 CCCGGG
XmaI 29 CCCGGG C^CCGGG
1556 CCCGGG
XmnI 308 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1192 GAACAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspHI
BsrBI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PaeR7I
PleI PpuMI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoI XhoII