##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I AspEI AviII
AvrII BalI BanII BbeI BbrPI
BbvCI BcgI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI CfoI
Cfr10I ClaI CpoI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KspI MamI MluI MluNI
MroI MscI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I Van91I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 51 GGTACC G^GTACC
AflII 1956 CTTAAG C^TTAAG
AseI 234 ATTAAT AT^TAAT
AsnI 234 ATTAAT AT^TAAT
Asp718I 51 GGTACC G^GTACC
AspI 103 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1828 CTCGGG C^[CT]CG[GA]G
BamHI 2895 GGATCC G^GATCC
BcnI 161 CCGGG CC^[GC]GG
BfrI 1956 CTTAAG C^TTAAG
BlpI 1082 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1082 GCTCAGC GC^T[ACGT]AGC
BsgI 961 GTGCAG GTGCAG(16/14)
BsmFI 165 GTCCC GGGAC(10/14)
BsmI 1880 GAATGC GAATGC(1/-1)
BsoBI 1828 CTCGGG C^[CT]CG[GA]G
BspMI 1333 GCAGGT ACCTGC(4/8)
Bst1107I 2626 GTATAC GTA^TAC
BstXI 2409 CCAATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2626 GTATAC GTA^TAC
CelII 1082 GCTCAGC GC^T[ACGT]AGC
DraII 163 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 2211 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2269 CCACGG C^C[GA][CT]GG
EarI 3152 CTCTTC CTCTTC(1/4)
EcoO109I 163 GGGTCCC [GA]G^G[ACGT]CC[CT]
HapII 161 CCGG C^CGG
HpaII 161 CCGG C^CGG
KpnI 51 GGTACC GGTAC^C
Ksp632I 3152 CTCTTC CTCTTC(1/4)
MspI 161 CCGG C^CGG
NciI 161 CCGGG CC^[GC]GG
NdeI 894 CATATG CA^TATG
PflFI 103 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 163 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 234 ATTAAT AT^TAAT
ScaI 1621 AGTACT AGT^ACT
StuI 539 AGGCCT AGG^CCT
SwaI 1529 ATTTAAAT ATTT^AAAT
Tth111I 103 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2129 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 2083 GTAGAC GT^[AC][GT]AC
2626 GTATAC
AciI 2389 CCGC CCGC(-3/-1)
3197 CCGC
AflIII 1016 ACATGT A^C[GA][CT]GT
3422 ACATGT
AocI 59 CCTCAGG CC^T[ACGT]AGG
1764 CCTGAGG
AspHI 2841 GAGCAC G[AT]GC[AT]^C
3397 GAGCAC
AvaII 164 GGTCC G^G[AT]CC
1809 GGTCC
BbsI 493 GAAGAC GAAGAC(2/6)
457 GTCTTC
BciVI 1130 GGATAC GTATCC(6/5)
2234 GGATAC
BclI 3511 TGATCA T^GATCA
3549 TGATCA
BpmI 294 CTGGAG CTGGAG(16/14)
645 CTGGAG
BpuAI 493 GAAGAC GAAGAC(2/6)
457 GTCTTC
BsaI 3149 GGTCTC GGTCTC(1/5)
3165 GGTCTC
BseRI 57 CTCCTC GAGGAG(10/8)
648 GAGGAG
BsiHKAI 2841 GAGCAC G[AT]GC[AT]^C
3397 GAGCAC
BsiYI 55 CCCTCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
167 CCCTGGAAAGG
BslI 55 CCCTCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
167 CCCTGGAAAGG
Bsp1407I 480 TGTACA T^GTACA
1011 TGTACA
BspLU11I 1016 ACATGT A^CATGT
3422 ACATGT
BsrGI 480 TGTACA T^GTACA
1011 TGTACA
BstYI 1004 GGATCT [GA]^GATC[CT]
2895 GGATCC
Bsu36I 59 CCTCAGG CC^T[ACGT]AGG
1764 CCTGAGG
Eco81I 59 CCTCAGG CC^T[ACGT]AGG
1764 CCTGAGG
EcoRI 180 GAATTC G^AATTC
740 GAATTC
EcoT14I 835 CCAAGG C^C[AT][AT]GG
2278 CCTTGG
FbaI 3511 TGATCA T^GATCA
3549 TGATCA
MfeI 1963 CAATTG C^AATTG
2706 CAATTG
MflI 1004 GGATCT [GA]^GATC[CT]
2895 GGATCC
MunI 1963 CAATTG C^AATTG
2706 CAATTG
PleI 2065 GACTC GAGTC(4/5)
155 GAGTC
PstI 367 CTGCAG CTGCA^G
2338 CTGCAG
SspBI 480 TGTACA T^GTACA
1011 TGTACA
StyI 835 CCAAGG C^C[AT][AT]GG
2278 CCTTGG
XcmI 5 CCAGCTGTGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2269 CCACGGAATCCTTGG
XhoII 1004 GGATCT [GA]^GATC[CT]
2895 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI BanI BbvI BfaI BmyI
BsaJI BsmAI Bsp1286I BspHI BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI EcoRII
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I