##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Alw44I ApaLI AseI AsnI Asp700I
AviII AvrII BclI BfrI BglII
BlnI BsaBI BsiEI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KspI
MamI MfeI MluI MunI NaeI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SwaI XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 195 GGTACC G^GTACC
AcsI 998 GAATTT [GA]^AATT[CT]
AflIII 3797 ACGTGT A^C[GA][CT]GT
AhdI 1648 GACTCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2856 AGCGCT AGC^GCT
ApoI 998 GAATTT [GA]^AATT[CT]
AscI 1 GGCGCGCC GG^CGCGCC
Asp718I 195 GGTACC G^GTACC
AspEI 1648 GACTCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 835 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2918 GGCGCC GGCGC^C
BbrPI 3796 CACGTG CAC^GTG
BciVI 1333 GGATAC GTATCC(6/5)
BsaAI 3796 CACGTG [CT]AC^GT[GA]
BsrFI 192 GCCGGT [GA]^CCGG[CT]
BssHII 2 GCGCGC G^CGCGC
BstEII 3091 GGTGACC G^GT[ACGT]ACC
BstPI 3091 GGTGACC G^GT[ACGT]ACC
Cfr10I 192 GCCGGT [GA]^CCGG[CT]
DraIII 1688 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1648 GACTCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2856 AGCGCT AGC^GCT
EcoO65I 3091 GGTGACC G^GT[ACGT]ACC
KasI 2918 GGCGCC G^GCGCC
KpnI 195 GGTACC GGTAC^C
MseI 3997 TTAA T^TAA
MslI 508 CACGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2918 GGCGCC GG^CGCC
NdeI 3809 CATATG CA^TATG
PaeR7I 2334 CTCGAG C^TCGAG
PflFI 835 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3796 CACGTG CAC^GTG
PmlI 3796 CACGTG CAC^GTG
PshAI 417 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfiI 1946 GGCCTGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2918 GGCGCC GGC^GCC
SspI 3922 AATATT AAT^ATT
TaqI 2335 TCGA T^CGA
Tru9I 3997 TTAA T^TAA
Tth111I 835 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2335 TCGA T^CGA
XhoI 2334 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccIII 2712 TCCGGA T^CCGGA
2727 TCCGGA
AcyI 2918 GGCGCC G[GA]^CG[CT]C
3589 GACGCC
BamHI 2556 GGATCC G^GATCC
3380 GGATCC
BlpI 439 GCTGAGC GC^T[ACGT]AGC
747 GCTCAGC
Bpu1102I 439 GCTGAGC GC^T[ACGT]AGC
747 GCTCAGC
BsaHI 2918 GGCGCC G[GA]^CG[CT]C
3589 GACGCC
BsaI 423 GGTCTC GGTCTC(1/5)
1655 GGTCTC
BsaWI 2712 TCCGGA [AT]^CCGG[AT]
2727 TCCGGA
BseAI 2712 TCCGGA T^CCGGA
2727 TCCGGA
BsmI 1577 GCATTC GAATGC(1/-1)
3495 GCATTC
BspEI 2712 TCCGGA T^CCGGA
2727 TCCGGA
BssSI 3039 CACGAG CACGAG(-5/-1)
810 CTCGTG
CelII 439 GCTGAGC GC^T[ACGT]AGC
747 GCTCAGC
Hin1I 2918 GGCGCC G[GA]^CG[CT]C
3589 GACGCC
MroI 2712 TCCGGA T^CCGGA
2727 TCCGGA
PflMI 2000 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2449 CCATGTCCTGG
SacI 1231 GAGCTC GAGCT^C
2380 GAGCTC
SbfI 300 CCTGCAGG CCTGCA^GG
3309 CCTGCAGG
Sse8387I 300 CCTGCAGG CCTGCA^GG
3309 CCTGCAGG
Van91I 2000 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2449 CCATGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI TseI Tsp45I
Tsp509I TspRI XcmI XhoII