##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AflII AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BfrI BglII
BlnI BsaBI BsiEI BsiWI BspDI
BssHII Bst1107I BstZ17I ClaI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoT22I FseI FspI HindIII
HpaI KasI KpnI MamI MfeI
MluI MunI NarI NcoI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PmaCI PmeI PmlI PshBI
PvuI SbfI ScaI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 1622 AACGTT AA^CGTT
AcsI 2069 GAATTC [GA]^AATT[CT]
AgeI 3255 ACCGGT A^CCGGT
AocI 592 CCTGAGG CC^T[ACGT]AGG
ApoI 2069 GAATTC [GA]^AATT[CT]
BlpI 170 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 170 GCTGAGC GC^T[ACGT]AGC
BsmI 3731 GAATGC GAATGC(1/-1)
Bsp1407I 1143 TGTACA T^GTACA
BspHI 2925 TCATGA T^CATGA
BspLU11I 3672 ACATGT A^CATGT
BsrGI 1143 TGTACA T^GTACA
BstBI 2730 TTCGAA TT^CGAA
BstXI 1200 CCAACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 592 CCTGAGG CC^T[ACGT]AGG
CelII 170 GCTGAGC GC^T[ACGT]AGC
CpoI 1593 CGGACCG CG^G[AT]CCG
DraIII 2422 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 592 CCTGAGG CC^T[ACGT]AGG
EcoRI 2069 GAATTC G^AATTC
EcoRV 1976 GATATC GAT^ATC
KspI 2598 CCGCGG CCGC^GG
MseI 3525 TTAA T^TAA
NaeI 224 GCCGGC GCC^GGC
NgoMIV 224 GCCGGC G^CCGGC
NspV 2730 TTCGAA TT^CGAA
PinAI 3255 ACCGGT A^CCGGT
Psp1406I 1622 AACGTT AA^CGTT
RcaI 2925 TCATGA T^CATGA
RsrII 1593 CGGACCG CG^G[AT]CCG
SacI 3722 GAGCTC GAGCT^C
SacII 2598 CCGCGG CCGC^GG
SalI 2550 GTCGAC G^TCGAC
SapI 274 GCTCTTC GCTCTTC(1/4)
SfiI 634 GGCCATCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2730 TTCGAA TT^CGAA
SspBI 1143 TGTACA T^GTACA
TfiI 3768 GATTC G^A[AT]TC
Tru9I 3525 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1241 GTCTAC GT^[AC][GT]AC
2550 GTCGAC
AccIII 1367 TCCGGA T^CCGGA
1575 TCCGGA
ApaI 712 GGGCCC GGGCC^C
1250 GGGCCC
Asp700I 253 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1756 GAACAGCTTC
AvaI 38 CCCGAG C^[CT]CG[GA]G
151 CCCGAG
BanI 682 GGTGCC G^G[CT][GA]CC
1453 GGTGCC
BclI 720 TGATCA T^GATCA
2740 TGATCA
BfaI 3632 CTAG C^TAG
3780 CTAG
BglI 635 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2486 GCCAGCAAGGC
BsaAI 1165 CACGTA [CT]AC^GT[GA]
1436 TACGTG
BseAI 1367 TCCGGA T^CCGGA
1575 TCCGGA
BsgI 1744 CTGCAC GTGCAG(16/14)
2471 GTGCAG
BsmBI 1183 CGTCTC CGTCTC(1/5)
965 GAGACG
BsoBI 38 CCCGAG C^[CT]CG[GA]G
151 CCCGAG
BspEI 1367 TCCGGA T^CCGGA
1575 TCCGGA
BsrDI 17 CATTGC GCAATG(2/0)
1383 GCAATG
BssSI 3594 CACGAG CACGAG(-5/-1)
1283 CTCGTG
BstAPI 764 GCAGACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2248 GCAGCGTGTGC
BstYI 588 AGATCC [GA]^GATC[CT]
1288 GGATCT
FbaI 720 TGATCA T^GATCA
2740 TGATCA
HaeII 1647 GGCGCT [GA]GCGC^[CT]
2708 AGCGCC
MaeI 3632 CTAG C^TAG
3780 CTAG
MflI 588 AGATCC [GA]^GATC[CT]
1288 GGATCT
MroI 1367 TCCGGA T^CCGGA
1575 TCCGGA
MslI 1861 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2260 CACAGGCATG
NspI 2265 GCATGT [GA]CATG^[CT]
3672 ACATGT
SexAI 417 ACCAGGT A^CC[AT]GGT
546 ACCTGGT
SmlI 529 CTCAAG C^T[CT][GA]AG
1790 CTCAAG
XhoII 588 AGATCC [GA]^GATC[CT]
1288 GGATCT
XmnI 253 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1756 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AspHI
AspI AvaII BalI BanII BbsI
BbvCI BbvI BcnI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflFI
PflMI PleI PpuMI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I