##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AviII BbrPI BciVI BclI
BfrI BglII BlpI Bpu1102I BsaAI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraIII EcoO65I EcoRI EcoT22I
FbaI FspI HpaI KpnI MluI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SalI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AhdI 601 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 835 CCTCAGG CC^T[ACGT]AGG
Aor51HI 262 AGCGCT AGC^GCT
AspEI 601 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1068 CCTAGG C^CTAGG
BbsI 2644 GTCTTC GAAGAC(2/6)
BbvCI 4143 GCTGAGG CCTCAGC(-5/-2)
BcgI 3602 GCAAGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1068 CCTAGG C^CTAGG
BpuAI 2644 GTCTTC GAAGAC(2/6)
Bsu36I 835 CCTCAGG CC^T[ACGT]AGG
DraI 4481 TTTAAA TTT^AAA
DrdI 2763 GACTCCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 601 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 262 AGCGCT AGC^GCT
Eco81I 835 CCTCAGG CC^T[ACGT]AGG
EcoRV 3331 GATATC GAT^ATC
FseI 4351 GGCCGGCC GGCCGG^CC
HincII 317 GTCAAC GT[CT]^[GA]AC
HindII 317 GTCAAC GT[CT]^[GA]AC
KspI 143 CCGCGG CCGC^GG
MfeI 406 CAATTG C^AATTG
MslI 3006 CACAGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 406 CAATTG C^AATTG
NcoI 3383 CCATGG C^CATGG
PshAI 762 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2261 CAGCTG CAG^CTG
SacI 5153 GAGCTC GAGCT^C
SacII 143 CCGCGG CCGC^GG
SapI 4548 GCTCTTC GCTCTTC(1/4)
SbfI 940 CCTGCAGG CCTGCA^GG
SfiI 3513 GGCCAGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 940 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 3593 GACGTC GACGT^C
4393 GACGTC
AccI 2398 GTCTAC GT^[AC][GT]AC
3226 GTCTAC
AccIII 597 TCCGGA T^CCGGA
3750 TCCGGA
AcsI 49 AAATTC [GA]^AATT[CT]
5223 GAATTT
ApoI 49 AAATTC [GA]^AATT[CT]
5223 GAATTT
Asp700I 2115 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5053 GAAAGGTTTC
AspI 984 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2392 GACCATGTC
BalI 2225 TGGCCA TGG^CCA
3512 TGGCCA
BamHI 1032 GGATCC G^GATCC
2803 GGATCC
BsaBI 150 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3355 GATGTGGATC
BseAI 597 TCCGGA T^CCGGA
3750 TCCGGA
BsmBI 4694 CGTCTC CGTCTC(1/5)
5069 GAGACG
BspEI 597 TCCGGA T^CCGGA
3750 TCCGGA
BstXI 1819 CCATTATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3086 CCATCCTCCTGG
EagI 237 CGGCCG C^GGCCG
4467 CGGCCG
EclXI 237 CGGCCG C^GGCCG
4467 CGGCCG
Eco52I 237 CGGCCG C^GGCCG
4467 CGGCCG
HindIII 252 AAGCTT A^AGCTT
2536 AAGCTT
MamI 150 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3355 GATGTGGATC
MluNI 2225 TGGCCA TGG^CCA
3512 TGGCCA
MroI 597 TCCGGA T^CCGGA
3750 TCCGGA
MscI 2225 TGGCCA TGG^CCA
3512 TGGCCA
PaeR7I 697 CTCGAG C^TCGAG
4286 CTCGAG
PflFI 984 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2392 GACCATGTC
Tth111I 984 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2392 GACCATGTC
XhoI 697 CTCGAG C^TCGAG
4286 CTCGAG
XmnI 2115 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5053 GAAAGGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI