##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI AlwI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII BamHI BbeI BbrPI BcnI
BglI BlpI Bpu1102I BsaHI BsaWI
BseAI BsiEI BsiWI BsoBI BspDI
BspEI BspLU11I BspMI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV FauI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SpeI Sse8387I
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AccI 3432 GTCTAC GT^[AC][GT]AC
AflII 3383 CTTAAG C^TTAAG
AvrII 4599 CCTAGG C^CTAGG
BbvCI 3796 CCTCAGC CCTCAGC(-5/-2)
BcgI 4670 GCAAACATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4400 GGATAC GTATCC(6/5)
BfrI 3383 CTTAAG C^TTAAG
BlnI 4599 CCTAGG C^CTAGG
BsaAI 2588 TACGTA [CT]AC^GT[GA]
BsaBI 3880 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 4147 CGTCTC CGTCTC(1/5)
BsrBI 330 GAGCGG CCGCTC(-3/-3)
BsrDI 304 GCAATG GCAATG(2/0)
BsrFI 3822 ACCGGC [GA]^CCGG[CT]
Cfr10I 3822 ACCGGC [GA]^CCGG[CT]
DrdI 3967 GACTTCCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 346 ATGCAT ATGCA^T
HapII 3823 CCGG C^CGG
HpaII 3823 CCGG C^CGG
MamI 3880 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 3823 CCGG C^CGG
NsiI 346 ATGCAT ATGCA^T
PstI 419 CTGCAG CTGCA^G
SnaBI 2588 TACGTA TAC^GTA
SspI 4701 AATATT AAT^ATT
TaqI 3977 TCGA T^CGA
TthHB8I 3977 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 1608 GTGCAC G^TGCAC
2761 GTGCAC
ApaLI 1608 GTGCAC G^TGCAC
2761 GTGCAC
BalI 384 TGGCCA TGG^CCA
453 TGGCCA
BanI 1300 GGCACC G^G[CT][GA]CC
4582 GGCACC
BclI 566 TGATCA T^GATCA
4104 TGATCA
BsaI 3505 GAGACC GGTCTC(1/5)
3739 GGTCTC
Bsp1407I 4365 TGTACA T^GTACA
4555 TGTACA
BsrGI 4365 TGTACA T^GTACA
4555 TGTACA
BssSI 4089 CACGAG CACGAG(-5/-1)
4097 CACGAG
BstAPI 190 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4297 GCAACAAGTGC
BstXI 3806 CCAAAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4058 CCAGCACTTTGG
CfoI 1143 GCGC GCG^C
1722 GCGC
DsaI 387 CCATGG C^C[GA][CT]GG
3551 CCATGG
EaeI 384 TGGCCA [CT]^GGCC[GA]
453 TGGCCA
EcoNI 545 CCTTCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3802 CCTCCCAAAGG
FbaI 566 TGATCA T^GATCA
4104 TGATCA
HhaI 1143 GCGC GCG^C
1722 GCGC
HinP1I 1143 GCGC G^CGC
1722 GCGC
MluNI 384 TGGCCA TGG^CCA
453 TGGCCA
MscI 384 TGGCCA TGG^CCA
453 TGGCCA
MwoI 190 GCAAGGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
4297 GCAACAAGTGC
NcoI 387 CCATGG C^CATGG
3551 CCATGG
SfaNI 301 GATGC GCATC(5/9)
199 GCATC
SfcI 419 CTGCAG C^T[GA][CT]AG
4195 CTGTAG
SspBI 4365 TGTACA T^GTACA
4555 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI Asp700I AspHI AvaII BanII
BbsI BbvI BfaI BglII BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI NdeI NdeII NlaIII
NlaIV NspI PleI PpuMI RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SmlI SphI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmnI