##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AclI AflII AgeI Aor51HI
AscI AseI AsnI Asp718I AviII
BamHI BbrPI BfrI BlpI Bpu1102I
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrGI Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FseI FspI HpaI KpnI
KspI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 4919 GACGTC GACGT^C
AccI 4384 GTCTAC GT^[AC][GT]AC
AccIII 399 TCCGGA T^CCGGA
AflIII 1312 ACACGT A^C[GA][CT]GT
AhdI 2026 GACCTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1271 GTGCAC G^TGCAC
ApaI 1714 GGGCCC GGGCC^C
ApaLI 1271 GTGCAC G^TGCAC
AspEI 2026 GACCTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 828 GGCGCC GGCGC^C
BcgI 38 GCAGCTACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1268 TACGTG [CT]AC^GT[GA]
BsaBI 4704 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 399 TCCGGA T^CCGGA
BspEI 399 TCCGGA T^CCGGA
Eam1105I 2026 GACCTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2101 CCTTTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3780 GAATTC G^AATTC
HincII 3354 GTTGAC GT[CT]^[GA]AC
HindII 3354 GTTGAC GT[CT]^[GA]AC
HindIII 2472 AAGCTT A^AGCTT
KasI 828 GGCGCC G^GCGCC
MamI 4704 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 399 TCCGGA T^CCGGA
NaeI 4439 GCCGGC GCC^GGC
NarI 828 GGCGCC GG^CGCC
NgoMIV 4439 GCCGGC G^CCGGC
PshAI 4899 GACGACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2131 GAAGAGC GCTCTTC(1/4)
SbfI 3628 CCTGCAGG CCTGCA^GG
SfoI 828 GGCGCC GGC^GCC
SmlI 2197 CTCAAG C^T[CT][GA]AG
Sse8387I 3628 CCTGCAGG CCTGCA^GG
StuI 934 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvrII 232 CCTAGG C^CTAGG
2512 CCTAGG
BciVI 631 GGATAC GTATCC(6/5)
1844 GGATAC
BglII 442 AGATCT A^GATCT
1570 AGATCT
BlnI 232 CCTAGG C^CTAGG
2512 CCTAGG
BsaWI 399 TCCGGA [AT]^CCGG[AT]
1149 ACCGGA
BsiEI 1147 CGACCG CG[GA][CT]^CG
4904 CGGTCG
BsrBI 1811 CCGCTC CCGCTC(-3/-3)
1880 CCGCTC
BsrFI 3649 ACCGGC [GA]^CCGG[CT]
4439 GCCGGC
BssHII 58 GCGCGC G^CGCGC
3986 GCGCGC
BssSI 3253 CACGAG CACGAG(-5/-1)
1195 CTCGTG
Cfr10I 3649 ACCGGC [GA]^CCGG[CT]
4439 GCCGGC
NcoI 375 CCATGG C^CATGG
2049 CCATGG
PpuMI 605 GGGTCCC [GA]G^G[AT]CC[CT]
3551 GGGTCCT
PstI 328 CTGCAG CTGCA^G
3629 CTGCAG
SacI 1043 GAGCTC GAGCT^C
2587 GAGCTC
SphI 3073 GCATGC GCATG^C
4624 GCATGC
XbaI 2186 TCTAGA T^CTAGA
2575 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BclI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoO65I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI