##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AflII AseI AsnI BciVI BclI
BfrI BglII BsaBI BsmI BspDI
BspHI BspLU11I Bst1107I BstBI BstZ17I
ClaI CpoI DraI EcoRI EcoT22I
FbaI HindIII HpaI MamI MfeI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshBI RcaI RsrII SfuI SnaBI
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 4320 GACGTC GACGT^C
AclI 5391 AACGTT AA^CGTT
AcsI 5181 GAATTT [GA]^AATT[CT]
AgeI 1722 ACCGGT A^CCGGT
ApoI 5181 GAATTT [GA]^AATT[CT]
AscI 298 GGCGCGCC GG^CGCGCC
AspI 6135 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BsiWI 1255 CGTACG C^GTACG
Bsp1407I 1611 TGTACA T^GTACA
BsrDI 941 GCAATG GCAATG(2/0)
BsrGI 1611 TGTACA T^GTACA
BssSI 1361 CTCGTG CACGAG(-5/-1)
BstEII 484 GGTGACC G^GT[ACGT]ACC
BstPI 484 GGTGACC G^GT[ACGT]ACC
EcoO65I 484 GGTGACC G^GT[ACGT]ACC
EcoRV 6910 GATATC GAT^ATC
MluI 1984 ACGCGT A^CGCGT
PflFI 6135 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1722 ACCGGT A^CCGGT
PshAI 4215 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5391 AACGTT AA^CGTT
PvuI 3842 CGATCG CGAT^CG
SalI 3895 GTCGAC G^TCGAC
SapI 4554 GAAGAGC GCTCTTC(1/4)
ScaI 5406 AGTACT AGT^ACT
SfiI 1016 GGCCACACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 5855 ACTAGT A^CTAGT
SspBI 1611 TGTACA T^GTACA
Tth111I 6135 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1388 GTCTAC GT^[AC][GT]AC
3895 GTCGAC
AflIII 1984 ACGCGT A^C[GA][CT]GT
5442 ACGTGT
AhdI 1160 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7109 GACTCTGTGTC
AspEI 1160 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7109 GACTCTGTGTC
AvrII 5340 CCTAGG C^CTAGG
5663 CCTAGG
BamHI 2270 GGATCC G^GATCC
3248 GGATCC
BbrPI 1304 CACGTG CAC^GTG
3888 CACGTG
BcgI 853 CGATCTGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1233 CGAGCAGCATGC
BlnI 5340 CCTAGG C^CTAGG
5663 CCTAGG
BsaAI 1304 CACGTG [CT]AC^GT[GA]
3888 CACGTG
DrdI 1379 GACGGCTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4638 GACCGACTGGTC
EagI 837 CGGCCG C^GGCCG
1956 CGGCCG
Eam1105I 1160 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7109 GACTCTGTGTC
EclXI 837 CGGCCG C^GGCCG
1956 CGGCCG
Eco52I 837 CGGCCG C^GGCCG
1956 CGGCCG
FseI 4248 GGCCGGCC GGCCGG^CC
5334 GGCCGGCC
MseI 2325 TTAA T^TAA
6955 TTAA
PmaCI 1304 CACGTG CAC^GTG
3888 CACGTG
PmlI 1304 CACGTG CAC^GTG
3888 CACGTG
SgrAI 1194 CGCCGGTG C[GA]^CCGG[CT]G
4781 CGCCGGTG
SmlI 4150 CTCAAG C^T[CT][GA]AG
5402 CTCAAG
Tru9I 2325 TTAA T^TAA
6955 TTAA
XcmI 906 CCATCTCGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3599 CCAACCAGCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 162 enzymes) #
#########################################
Acc65I AccII AccIII AciI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI Asp700I
Asp718I AspHI AvaI AvaII AviII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrFI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI KpnI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I SbfI
ScrFI SexAI SfaNI SfcI SfoI
SmaI SphI Sse8387I StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI XmnI