##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AocI Aor51HI AscI Asp718I
AspEI BamHI BcgI BciVI BfrI
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BstBI Bsu36I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRV FseI KpnI
MluI MroI NaeI NgoMIV NheI
NotI NruI NspV PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacI SalI SapI SbfI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 1209 GACGTC GACGT^C
ApaI 415 GGGCCC GGGCC^C
Asp700I 5666 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 5059 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 997 TGCGCA TGC^GCA
AvrII 2166 CCTAGG C^CTAGG
BalI 5607 TGGCCA TGG^CCA
BbvCI 3643 CCTCAGC CCTCAGC(-5/-2)
BclI 5712 TGATCA T^GATCA
BglI 530 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2166 CCTAGG C^CTAGG
BsmBI 752 CGTCTC CGTCTC(1/5)
BssSI 1524 CACGAG CACGAG(-5/-1)
Bst1107I 6728 GTATAC GTA^TAC
BstEII 4865 GGTAACC G^GT[ACGT]ACC
BstPI 4865 GGTAACC G^GT[ACGT]ACC
BstZ17I 6728 GTATAC GTA^TAC
EaeI 5607 TGGCCA [CT]^GGCC[GA]
EcoO65I 4865 GGTAACC G^GT[ACGT]ACC
FbaI 5712 TGATCA T^GATCA
FspI 997 TGCGCA TGC^GCA
MfeI 2595 CAATTG C^AATTG
MluNI 5607 TGGCCA TGG^CCA
MscI 5607 TGGCCA TGG^CCA
MunI 2595 CAATTG C^AATTG
NdeI 2071 CATATG CA^TATG
PflFI 5059 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SmaI 576 CCCGGG CCC^GGG
Tth111I 5059 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 419 CCAGTCCCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 576 CCCGGG C^CCGGG
XmaI 576 CCCGGG C^CCGGG
XmnI 5666 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 4032 GTAGAC GT^[AC][GT]AC
6728 GTATAC
Alw44I 1470 GTGCAC G^TGCAC
1668 GTGCAC
AlwI 3959 GGATC GGATC(4/5)
5397 GGATC
ApaLI 1470 GTGCAC G^TGCAC
1668 GTGCAC
AvaI 576 CCCGGG C^[CT]CG[GA]G
1554 CTCGGG
BbsI 2617 GAAGAC GAAGAC(2/6)
6436 GTCTTC
BglII 2553 AGATCT A^GATCT
3969 AGATCT
BpuAI 2617 GAAGAC GAAGAC(2/6)
6436 GTCTTC
BsaBI 194 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1623 GATTTGCATC
BsaWI 649 ACCGGA [AT]^CCGG[AT]
1274 ACCGGA
BsoBI 576 CCCGGG C^[CT]CG[GA]G
1554 CTCGGG
BssHII 892 GCGCGC G^CGCGC
894 GCGCGC
BstAPI 818 GCACGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2564 GCAGCTTTTGC
BstXI 3788 CCAAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5112 CCATGTGTCTGG
EcoNI 779 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2235 CCTGGCAAAGG
EcoRI 4510 GAATTC G^AATTC
6581 GAATTC
EcoT22I 2146 ATGCAT ATGCA^T
5810 ATGCAT
HindIII 6371 AAGCTT A^AGCTT
6636 AAGCTT
KspI 833 CCGCGG CCGC^GG
989 CCGCGG
MamI 194 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1623 GATTTGCATC
NcoI 1968 CCATGG C^CATGG
3104 CCATGG
NsiI 2146 ATGCAT ATGCA^T
5810 ATGCAT
PacI 3059 TTAATTAA TTAAT^TAA
3144 TTAATTAA
SacII 833 CCGCGG CCGC^GG
989 CCGCGG
ScaI 4198 AGTACT AGT^ACT
4345 AGTACT
SexAI 4880 ACCTGGT A^CC[AT]GGT
5060 ACCAGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwNI ApoI AseI
AsnI AspHI AvaII BanI BanII
BbeI BbrPI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PshBI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII