##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
Asp718I AvrII BbrPI BbsI BfrI
BlnI BpuAI BsaAI BsaBI BseAI
BsiWI BspDI BspEI BsrDI BstAPI
BstBI BstXI ClaI CpoI DraIII
EcoRV EcoT22I FseI HincII HindII
HpaI KpnI MamI MluI MroI
NcoI NdeI NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 2016 GTATAC GT^[AC][GT]AC
AgeI 2193 ACCGGT A^CCGGT
AhdI 2158 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3497 GTGCAC G^TGCAC
Aor51HI 670 AGCGCT AGC^GCT
ApaLI 3497 GTGCAC G^TGCAC
AscI 898 GGCGCGCC GG^CGCGCC
AspEI 2158 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3519 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4013 TGCGCA TGC^GCA
BcgI 287 CGAGTTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3022 TGATCA T^GATCA
BpmI 1599 CTGGAG CTGGAG(16/14)
BsmBI 267 GAGACG CGTCTC(1/5)
Bsp1407I 3916 TGTACA T^GTACA
BspHI 1058 TCATGA T^CATGA
BspMI 1674 GCAGGT ACCTGC(4/8)
BsrGI 3916 TGTACA T^GTACA
Bst1107I 2016 GTATAC GTA^TAC
BstEII 3880 GGTGACC G^GT[ACGT]ACC
BstPI 3880 GGTGACC G^GT[ACGT]ACC
BstZ17I 2016 GTATAC GTA^TAC
Eam1105I 2158 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 670 AGCGCT AGC^GCT
EcoO65I 3880 GGTGACC G^GT[ACGT]ACC
EcoRI 975 GAATTC G^AATTC
FbaI 3022 TGATCA T^GATCA
FspI 4013 TGCGCA TGC^GCA
PflFI 3519 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2193 ACCGGT A^CCGGT
PstI 2399 CTGCAG CTGCA^G
RcaI 1058 TCATGA T^CATGA
SacI 1519 GAGCTC GAGCT^C
SapI 2760 GCTCTTC GCTCTTC(1/4)
ScaI 160 AGTACT AGT^ACT
SmlI 3278 CTCAAG C^T[CT][GA]AG
SphI 1653 GCATGC GCATG^C
SspBI 3916 TGTACA T^GTACA
Tth111I 3519 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1515 CCAGGAGCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AocI 1585 CCTGAGG CC^T[ACGT]AGG
2368 CCTCAGG
Asp700I 536 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
971 GAATGAATTC
BalI 675 TGGCCA TGG^CCA
2500 TGGCCA
BamHI 583 GGATCC G^GATCC
3166 GGATCC
BciVI 3244 GTATCC GTATCC(6/5)
3673 GTATCC
BglII 601 AGATCT A^GATCT
1750 AGATCT
BlpI 1832 GCTCAGC GC^T[ACGT]AGC
2662 GCTCAGC
Bpu1102I 1832 GCTCAGC GC^T[ACGT]AGC
2662 GCTCAGC
BsaWI 2193 ACCGGT [AT]^CCGG[AT]
2733 TCCGGT
BsmI 2709 GCATTC GAATGC(1/-1)
3404 GCATTC
BstF5I 293 GGATG GGATG(2/0)
802 GGATG
Bsu36I 1585 CCTGAGG CC^T[ACGT]AGG
2368 CCTCAGG
CelII 1832 GCTCAGC GC^T[ACGT]AGC
2662 GCTCAGC
DrdI 1116 GACAATAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3555 GACATAAGGGTC
Eco81I 1585 CCTGAGG CC^T[ACGT]AGG
2368 CCTCAGG
EcoNI 2343 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4080 CCTCTCTGAGG
FokI 293 GGATG GGATG(9/13)
802 GGATG
HindIII 3887 AAGCTT A^AGCTT
3968 AAGCTT
MfeI 1850 CAATTG C^AATTG
4233 CAATTG
MluNI 675 TGGCCA TGG^CCA
2500 TGGCCA
MscI 675 TGGCCA TGG^CCA
2500 TGGCCA
MunI 1850 CAATTG C^AATTG
4233 CAATTG
NheI 57 GCTAGC G^CTAGC
3310 GCTAGC
PflMI 318 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3315 CCACAATTTGG
StuI 1576 AGGCCT AGG^CCT
2365 AGGCCT
TaqI 514 TCGA T^CGA
1766 TCGA
TthHB8I 514 TCGA T^CGA
1766 TCGA
Van91I 318 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3315 CCACAATTTGG
XmnI 536 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
971 GAATGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AseI AsnI AspHI AvaI
AvaII BanI BanII BbeI BbvCI
BbvI BcnI BfaI BglI BmyI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BsrBI
BsrFI BsrI BssHII BssKI BssSI
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PshBI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmaCI XmaI