##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AccI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AvrII BfrI BlnI BsiEI
BsiWI Bsp1407I BspDI BsrBI BsrGI
BssHII Bst1107I BstZ17I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FseI
KpnI MluI NaeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PshBI PvuI RsrII
SalI SapI SbfI SexAI SfiI
SgrAI SnaBI Sse8387I SspBI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 351 GACGTC GACGT^C
AccIII 2590 TCCGGA T^CCGGA
AclI 376 AACGTT AA^CGTT
AcsI 472 AAATTC [GA]^AATT[CT]
Alw44I 297 GTGCAC G^TGCAC
AocI 4420 CCTGAGG CC^T[ACGT]AGG
ApaLI 297 GTGCAC G^TGCAC
ApoI 472 AAATTC [GA]^AATT[CT]
Asp700I 3133 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3712 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3245 TGCGCA TGC^GCA
BamHI 2857 GGATCC G^GATCC
BciVI 1249 GGATAC GTATCC(6/5)
BglII 4176 AGATCT A^GATCT
BsaBI 1152 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2590 TCCGGA [AT]^CCGG[AT]
BseAI 2590 TCCGGA T^CCGGA
BspEI 2590 TCCGGA T^CCGGA
BssSI 2819 CTCGTG CACGAG(-5/-1)
BstBI 1402 TTCGAA TT^CGAA
BstEII 4958 GGTCACC G^GT[ACGT]ACC
BstPI 4958 GGTCACC G^GT[ACGT]ACC
Bsu36I 4420 CCTGAGG CC^T[ACGT]AGG
Eco81I 4420 CCTGAGG CC^T[ACGT]AGG
EcoNI 4389 CCTTTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4958 GGTCACC G^GT[ACGT]ACC
FspI 3245 TGCGCA TGC^GCA
HgaI 3052 GCGTC GACGC(5/10)
HpaI 710 GTTAAC GTT^AAC
KspI 4756 CCGCGG CCGC^GG
MamI 1152 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2590 TCCGGA T^CCGGA
NdeI 3516 CATATG CA^TATG
NspV 1402 TTCGAA TT^CGAA
PflFI 3712 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4585 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 376 AACGTT AA^CGTT
SacII 4756 CCGCGG CCGC^GG
ScaI 3097 AGTACT AGT^ACT
SfuI 1402 TTCGAA TT^CGAA
SpeI 2624 ACTAGT A^CTAGT
SspI 418 AATATT AAT^ATT
Tth111I 3712 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4585 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3453 TCTAGA T^CTAGA
XmnI 3133 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 4316 CGCG CG^CG
4757 CGCG
AflIII 60 ACATGT A^C[GA][CT]GT
1626 ACATGT
AlwNI 4586 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
5199 CAGCAGCTG
ApaI 3691 GGGCCC GGGCC^C
4902 GGGCCC
BbrPI 3338 CACGTG CAC^GTG
4691 CACGTG
BbvCI 3569 CCTCAGC CCTCAGC(-5/-2)
4726 CCTCAGC
BcgI 1991 CGACTCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2199 CGACCTAGCTGC
BclI 290 TGATCA T^GATCA
3388 TGATCA
BglI 1195 GCCGTTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4833 GCCCCAGGGGC
BlpI 2991 GCTTAGC GC^T[ACGT]AGC
3550 GCTGAGC
Bpu1102I 2991 GCTTAGC GC^T[ACGT]AGC
3550 GCTGAGC
BsaAI 3338 CACGTG [CT]AC^GT[GA]
4691 CACGTG
BspHI 147 TCATGA T^CATGA
928 TCATGA
BspLU11I 60 ACATGT A^CATGT
1626 ACATGT
BstUI 4316 CGCG CG^CG
4757 CGCG
BstXI 75 CCATCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4789 CCAAGCCCCTGG
CelII 2991 GCTTAGC GC^T[ACGT]AGC
3550 GCTGAGC
DraIII 1443 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
2569 CACTCAGTG
FbaI 290 TGATCA T^GATCA
3388 TGATCA
HincII 710 GTTAAC GT[CT]^[GA]AC
2160 GTTGAC
HindII 710 GTTAAC GT[CT]^[GA]AC
2160 GTTGAC
HindIII 2870 AAGCTT A^AGCTT
3134 AAGCTT
MvnI 4316 CGCG CG^CG
4757 CGCG
NcoI 1510 CCATGG C^CATGG
1771 CCATGG
PmaCI 3338 CACGTG CAC^GTG
4691 CACGTG
PmlI 3338 CACGTG CAC^GTG
4691 CACGTG
PpuMI 3160 AGGACCT [GA]G^G[AT]CC[CT]
4515 AGGACCT
PshAI 222 GACTATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
970 GACTGCTGTC
RcaI 147 TCATGA T^CATGA
928 TCATGA
SacI 3280 GAGCTC GAGCT^C
3753 GAGCTC
SmaI 3731 CCCGGG CCC^GGG
4565 CCCGGG
StuI 960 AGGCCT AGG^CCT
2396 AGGCCT
XmaCI 3731 CCCGGG C^CCGGG
4565 CCCGGG
XmaI 3731 CCCGGG C^CCGGG
4565 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII