##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AscI AseI AsnI Asp718I AviII
AvrII BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaBI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SexAI SfiI
SfuI SnaBI SpeI SspBI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1486 GTCTAC GT^[AC][GT]AC
AgeI 1674 ACCGGT A^CCGGT
AhdI 1561 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3957 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1241 AGCGCT AGC^GCT
AspEI 1561 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3319 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3123 GGCGCC GGCGC^C
BbvCI 112 GCTGAGG CCTCAGC(-5/-2)
BcgI 2559 GCAGGTCGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 4025 GAGACC GGTCTC(1/5)
BspLU11I 764 ACATGT A^CATGT
BstAPI 5 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3957 CCTGAGG CC^T[ACGT]AGG
Eam1105I 1561 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1241 AGCGCT AGC^GCT
Eco81I 3957 CCTGAGG CC^T[ACGT]AGG
EcoNI 1493 CCTCCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 1779 GTCAAC GT[CT]^[GA]AC
HindII 1779 GTCAAC GT[CT]^[GA]AC
KasI 3123 GGCGCC G^GCGCC
NaeI 2878 GCCGGC GCC^GGC
NarI 3123 GGCGCC GG^CGCC
NgoMIV 2878 GCCGGC G^CCGGC
NheI 1732 GCTAGC G^CTAGC
PflFI 3319 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1674 ACCGGT A^CCGGT
ScaI 1006 AGTACT AGT^ACT
SfoI 3123 GGCGCC GGC^GCC
SmlI 2382 CTTGAG C^T[CT][GA]AG
SphI 695 GCATGC GCATG^C
SspI 2302 AATATT AAT^ATT
Tth111I 3319 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 3123 GGCGCC G[GA]^CG[CT]C
4177 GGCGTC
Alw44I 3298 GTGCAC G^TGCAC
3499 GTGCAC
ApaLI 3298 GTGCAC G^TGCAC
3499 GTGCAC
BamHI 1413 GGATCC G^GATCC
3824 GGATCC
BglII 395 AGATCT A^GATCT
2197 AGATCT
BsaHI 3123 GGCGCC G[GA]^CG[CT]C
4177 GGCGTC
BsmBI 159 CGTCTC CGTCTC(1/5)
3324 CGTCTC
BsmI 1098 GAATGC GAATGC(1/-1)
1191 GAATGC
BsrDI 2210 CATTGC GCAATG(2/0)
3599 GCAATG
BssSI 3445 CACGAG CACGAG(-5/-1)
133 CTCGTG
Hin1I 3123 GGCGCC G[GA]^CG[CT]C
4177 GGCGTC
HindIII 1690 AAGCTT A^AGCTT
1758 AAGCTT
MseI 1205 TTAA T^TAA
2291 TTAA
PshAI 1529 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2019 GACGCTCGTC
SacI 884 GAGCTC GAGCT^C
1261 GAGCTC
SapI 1831 GAAGAGC GCTCTTC(1/4)
2986 GAAGAGC
SbfI 723 CCTGCAGG CCTGCA^GG
2496 CCTGCAGG
SgrAI 1673 CACCGGTG C[GA]^CCGG[CT]G
2877 CGCCGGCG
SmaI 57 CCCGGG CCC^GGG
2679 CCCGGG
Sse8387I 723 CCTGCAGG CCTGCA^GG
2496 CCTGCAGG
Tru9I 1205 TTAA T^TAA
2291 TTAA
XmaCI 57 CCCGGG C^CCGGG
2679 CCCGGG
XmaI 57 CCCGGG C^CCGGG
2679 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI