##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AscI AseI AsnI
Asp718I AspI AvrII BbrPI BciVI
BclI BfaI BfrI BglII BlnI
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI EcoRI EcoRV EcoT22I FbaI
HindIII HpaI KpnI MaeI MamI
MluI MroI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I RcaI RsrII SalI SapI
SexAI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 271 GAATTT [GA]^AATT[CT]
AhdI 3061 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 847 GTGCAC G^TGCAC
Aor51HI 2976 AGCGCT AGC^GCT
ApaLI 847 GTGCAC G^TGCAC
ApoI 271 GAATTT [GA]^AATT[CT]
Asp700I 4437 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3061 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2387 TGGCCA TGG^CCA
BamHI 971 GGATCC G^GATCC
BcgI 4457 CGACCCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 844 TACGTG [CT]AC^GT[GA]
BsaWI 3519 ACCGGA [AT]^CCGG[AT]
BsmI 831 GCATTC GAATGC(1/-1)
BssSI 3480 CACGAG CACGAG(-5/-1)
BstAPI 1372 GCACACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 971 GGATCC [GA]^GATC[CT]
DraI 4913 TTTAAA TTT^AAA
DraIII 1373 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3061 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2976 AGCGCT AGC^GCT
FseI 1521 GGCCGGCC GGCCGG^CC
MfeI 1273 CAATTG C^AATTG
MflI 971 GGATCC [GA]^GATC[CT]
MluNI 2387 TGGCCA TGG^CCA
MscI 2387 TGGCCA TGG^CCA
MseI 4914 TTAA T^TAA
MunI 1273 CAATTG C^AATTG
NcoI 2222 CCATGG C^CATGG
NspI 1161 GCATGT [GA]CATG^[CT]
PleI 2179 GACTC GAGTC(4/5)
PshAI 2572 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3185 CGATCG CGAT^CG
SbfI 465 CCTGCAGG CCTGCA^GG
ScaI 1670 AGTACT AGT^ACT
Sse8387I 465 CCTGCAGG CCTGCA^GG
StuI 2403 AGGCCT AGG^CCT
Tru9I 4914 TTAA T^TAA
XhoII 971 GGATCC [GA]^GATC[CT]
XmnI 4437 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AocI 1759 CCTCAGG CC^T[ACGT]AGG
3709 CCTCAGG
AviII 866 TGCGCA TGC^GCA
899 TGCGCA
BlpI 1878 GCTGAGC GC^T[ACGT]AGC
4170 GCTGAGC
Bpu1102I 1878 GCTGAGC GC^T[ACGT]AGC
4170 GCTGAGC
BstEII 1071 GGTGACC G^GT[ACGT]ACC
1461 GGTCACC
BstPI 1071 GGTGACC G^GT[ACGT]ACC
1461 GGTCACC
Bsu36I 1759 CCTCAGG CC^T[ACGT]AGG
3709 CCTCAGG
CelII 1878 GCTGAGC GC^T[ACGT]AGC
4170 GCTGAGC
Eco81I 1759 CCTCAGG CC^T[ACGT]AGG
3709 CCTCAGG
EcoNI 2655 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4933 CCTTTTGGAGG
EcoO65I 1071 GGTGACC G^GT[ACGT]ACC
1461 GGTCACC
FspI 866 TGCGCA TGC^GCA
899 TGCGCA
HincII 698 GTCAAC GT[CT]^[GA]AC
4209 GTCAAC
HindII 698 GTCAAC GT[CT]^[GA]AC
4209 GTCAAC
MaeII 845 ACGT A^CGT
4127 ACGT
PaeR7I 770 CTCGAG C^TCGAG
1489 CTCGAG
PvuII 1792 CAGCTG CAG^CTG
2641 CAGCTG
XhoI 770 CTCGAG C^TCGAG
1489 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvCI BbvI BcnI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PpuMI PstI
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SfoI
SmaI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XmaCI XmaI