##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AclI AgeI Aor51HI AscI
AseI AsnI AvrII BbeI BlnI
BsiWI BspDI BspLU11I BsrFI BssHII
BstBI Cfr10I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FseI HpaI KasI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AhdI 2565 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2828 GTGCAC G^TGCAC
AocI 2546 CCTCAGG CC^T[ACGT]AGG
ApaI 1984 GGGCCC GGGCC^C
ApaLI 2828 GTGCAC G^TGCAC
AspEI 2565 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1929 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 78 GCTGAGG CCTCAGC(-5/-2)
BcgI 2620 CGATCCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiEI 2765 CGACCG CG[GA][CT]^CG
BsmI 3958 GAATGC GAATGC(1/-1)
Bst1107I 4450 GTATAC GTA^TAC
BstZ17I 4450 GTATAC GTA^TAC
Bsu36I 2546 CCTCAGG CC^T[ACGT]AGG
Eam1105I 2565 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2546 CCTCAGG CC^T[ACGT]AGG
HaeII 75 GGCGCT [GA]GCGC^[CT]
HindIII 4436 AAGCTT A^AGCTT
KspI 1519 CCGCGG CCGC^GG
NcoI 2002 CCATGG C^CATGG
PaeR7I 4935 CTCGAG C^TCGAG
PflFI 1929 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 357 GAGCTC GAGCT^C
SacII 1519 CCGCGG CCGC^GG
SmaI 2152 CCCGGG CCC^GGG
SphI 1970 GCATGC GCATG^C
Tth111I 1929 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4935 CTCGAG C^TCGAG
XmaCI 2152 CCCGGG C^CCGGG
XmaI 2152 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1191 GGTACC G^GTACC
1501 GGTACC
AccI 402 GTCTAC GT^[AC][GT]AC
4450 GTATAC
AccIII 435 TCCGGA T^CCGGA
2472 TCCGGA
AcyI 1961 GACGCC G[GA]^CG[CT]C
4615 GGCGTC
AflII 4439 CTTAAG C^TTAAG
4544 CTTAAG
AflIII 1767 ACGTGT A^C[GA][CT]GT
4454 ACGTGT
Asp700I 2358 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2417 GAATAAGTTC
Asp718I 1191 GGTACC G^GTACC
1501 GGTACC
AviII 637 TGCGCA TGC^GCA
3240 TGCGCA
BamHI 895 GGATCC G^GATCC
1592 GGATCC
BbrPI 972 CACGTG CAC^GTG
1766 CACGTG
BbsI 2686 GAAGAC GAAGAC(2/6)
3696 GAAGAC
BclI 2432 TGATCA T^GATCA
3163 TGATCA
BfrI 4439 CTTAAG C^TTAAG
4544 CTTAAG
BglII 2407 AGATCT A^GATCT
4575 AGATCT
BlpI 1166 GCTCAGC GC^T[ACGT]AGC
1352 GCTGAGC
Bpu1102I 1166 GCTCAGC GC^T[ACGT]AGC
1352 GCTGAGC
BpuAI 2686 GAAGAC GAAGAC(2/6)
3696 GAAGAC
BsaBI 239 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2361 GATTTTCATC
BsaHI 1961 GACGCC G[GA]^CG[CT]C
4615 GGCGTC
BsaI 2135 GAGACC GGTCTC(1/5)
3212 GAGACC
BseAI 435 TCCGGA T^CCGGA
2472 TCCGGA
BspEI 435 TCCGGA T^CCGGA
2472 TCCGGA
BspHI 4340 TCATGA T^CATGA
4370 TCATGA
BssSI 2721 CACGAG CACGAG(-5/-1)
1016 CTCGTG
BstAPI 1699 GCAGGTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2821 GCACAGTGTGC
BstEII 885 GGTGACC G^GT[ACGT]ACC
1236 GGTGACC
BstPI 885 GGTGACC G^GT[ACGT]ACC
1236 GGTGACC
CelII 1166 GCTCAGC GC^T[ACGT]AGC
1352 GCTGAGC
DraI 3103 TTTAAA TTT^AAA
5421 TTTAAA
DraIII 136 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2822 CACAGTGTG
EcoO65I 885 GGTGACC G^GT[ACGT]ACC
1236 GGTGACC
FbaI 2432 TGATCA T^GATCA
3163 TGATCA
FspI 637 TGCGCA TGC^GCA
3240 TGCGCA
Hin1I 1961 GACGCC G[GA]^CG[CT]C
4615 GGCGTC
HincII 546 GTCAAC GT[CT]^[GA]AC
3676 GTCAAC
HindII 546 GTCAAC GT[CT]^[GA]AC
3676 GTCAAC
KpnI 1191 GGTACC GGTAC^C
1501 GGTACC
MamI 239 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2361 GATTTTCATC
MroI 435 TCCGGA T^CCGGA
2472 TCCGGA
PflMI 1251 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1788 CCACAGTTTGG
PmaCI 972 CACGTG CAC^GTG
1766 CACGTG
PmlI 972 CACGTG CAC^GTG
1766 CACGTG
RcaI 4340 TCATGA T^CATGA
4370 TCATGA
SexAI 1822 ACCAGGT A^CC[AT]GGT
4536 ACCTGGT
Van91I 1251 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1788 CCACAGTTTGG
XbaI 4018 TCTAGA T^CTAGA
4555 TCTAGA
XmnI 2358 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2417 GAATAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvI
BciVI BcnI BfaI BglI BmyI
BpmI BsaAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII