################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AclI AflII AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvrII BbrPI BclI BfrI BlnI BsiWI Bsp1407I BspDI BspHI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoRI EcoT22I FbaI FseI HpaI MfeI MluI MunI NdeI NheI NotI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 970 GGTACC G^GTACC AccIII 401 TCCGGA T^CCGGA AflIII 1784 ACATGT A^C[GA][CT]GT AgeI 1933 ACCGGT A^CCGGT Asp718I 970 GGTACC G^GTACC BbsI 1927 GTCTTC GAAGAC(2/6) BcgI 2859 CGACATTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1057 GTATCC GTATCC(6/5) BlpI 1256 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1256 GCTGAGC GC^T[ACGT]AGC BpuAI 1927 GTCTTC GAAGAC(2/6) BsaAI 3386 CACGTA [CT]AC^GT[GA] BsaBI 318 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 401 TCCGGA T^CCGGA BsiEI 21 CGGCCG CG[GA][CT]^CG BsmI 2882 GAATGC GAATGC(1/-1) BspEI 401 TCCGGA T^CCGGA BspLU11I 1784 ACATGT A^CATGT BsrDI 1877 CATTGC GCAATG(2/0) BssSI 1128 CTCGTG CACGAG(-5/-1) BstEII 368 GGTCACC G^GT[ACGT]ACC BstPI 368 GGTCACC G^GT[ACGT]ACC CelII 1256 GCTGAGC GC^T[ACGT]AGC DraIII 3556 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 21 CGGCCG C^GGCCG EclXI 21 CGGCCG C^GGCCG Eco52I 21 CGGCCG C^GGCCG EcoO65I 368 GGTCACC G^GT[ACGT]ACC EcoRV 415 GATATC GAT^ATC HincII 1461 GTCAAC GT[CT]^[GA]AC HindII 1461 GTCAAC GT[CT]^[GA]AC KpnI 970 GGTACC GGTAC^C MamI 318 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 401 TCCGGA T^CCGGA NaeI 19 GCCGGC GCC^GGC NgoMIV 19 GCCGGC G^CCGGC NruI 1695 TCGCGA TCG^CGA PinAI 1933 ACCGGT A^CCGGT SexAI 2239 ACCTGGT A^CC[AT]GGT SphI 13 GCATGC GCATG^C ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AatII 3087 GACGTC GACGT^C 3906 GACGTC AccI 3090 GTCTAC GT^[AC][GT]AC 3576 GTCTAC AcsI 2696 AAATTT [GA]^AATT[CT] 2919 AAATTT ApoI 2696 AAATTT [GA]^AATT[CT] 2919 AAATTT AviII 10 TGCGCA TGC^GCA 2258 TGCGCA BbeI 111 GGCGCC GGCGC^C 3313 GGCGCC BfaI 458 CTAG C^TAG 2379 CTAG BsaI 1041 GAGACC GGTCTC(1/5) 2436 GAGACC BsmBI 175 GAGACG CGTCTC(1/5) 1650 GAGACG BsrBI 3012 GAGCGG CCGCTC(-3/-3) 3330 GAGCGG BstXI 663 CCACCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2107 CCAAGGCTGTGG FspI 10 TGCGCA TGC^GCA 2258 TGCGCA HgaI 177 GACGC GACGC(5/10) 1652 GACGC HindIII 1408 AAGCTT A^AGCTT 3720 AAGCTT KasI 111 GGCGCC G^GCGCC 3313 GGCGCC KspI 105 CCGCGG CCGC^GG 276 CCGCGG MaeI 458 CTAG C^TAG 2379 CTAG MseI 850 TTAA T^TAA 1479 TTAA NarI 111 GGCGCC GG^CGCC 3313 GGCGCC NspI 13 GCATGC [GA]CATG^[CT] 1784 ACATGT SacII 105 CCGCGG CCGC^GG 276 CCGCGG SfoI 111 GGCGCC GGC^GCC 3313 GGCGCC SmaI 1945 CCCGGG CCC^GGG 3343 CCCGGG Tru9I 850 TTAA T^TAA 1479 TTAA XmaCI 1945 CCCGGG C^CCGGG 3343 CCCGGG XmaI 1945 CCCGGG C^CCGGG 3343 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BamHI BanI BanII BbvCI BbvI BcnI BglI BglII BmyI BpmI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII