##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AclI        AflII       AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AvrII       BbrPI       BclI        
BfrI        BlnI        BsiWI       Bsp1407I    BspDI       
BspHI       BsrGI       Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        DraI        Eam1105I    Eco47III    
EcoRI       EcoT22I     FbaI        FseI        HpaI        
MfeI        MluI        MunI        NdeI        NheI        
NotI        NsiI        NspV        PacI        PaeR7I      
PflFI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SbfI        ScaI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I        970  GGTACC               G^GTACC
AccIII        401  TCCGGA               T^CCGGA
AflIII       1784  ACATGT               A^C[GA][CT]GT
AgeI         1933  ACCGGT               A^CCGGT
Asp718I       970  GGTACC               G^GTACC
BbsI         1927  GTCTTC               GAAGAC(2/6)
BcgI         2859  CGACATTTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1057  GTATCC               GTATCC(6/5)
BlpI         1256  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1256  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1927  GTCTTC               GAAGAC(2/6)
BsaAI        3386  CACGTA               [CT]AC^GT[GA]
BsaBI         318  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         401  TCCGGA               T^CCGGA
BsiEI          21  CGGCCG               CG[GA][CT]^CG
BsmI         2882  GAATGC               GAATGC(1/-1)
BspEI         401  TCCGGA               T^CCGGA
BspLU11I     1784  ACATGT               A^CATGT
BsrDI        1877  CATTGC               GCAATG(2/0)
BssSI        1128  CTCGTG               CACGAG(-5/-1)
BstEII        368  GGTCACC              G^GT[ACGT]ACC
BstPI         368  GGTCACC              G^GT[ACGT]ACC
CelII        1256  GCTGAGC              GC^T[ACGT]AGC
DraIII       3556  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           21  CGGCCG               C^GGCCG
EclXI          21  CGGCCG               C^GGCCG
Eco52I         21  CGGCCG               C^GGCCG
EcoO65I       368  GGTCACC              G^GT[ACGT]ACC
EcoRV         415  GATATC               GAT^ATC
HincII       1461  GTCAAC               GT[CT]^[GA]AC
HindII       1461  GTCAAC               GT[CT]^[GA]AC
KpnI          970  GGTACC               GGTAC^C
MamI          318  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          401  TCCGGA               T^CCGGA
NaeI           19  GCCGGC               GCC^GGC
NgoMIV         19  GCCGGC               G^CCGGC
NruI         1695  TCGCGA               TCG^CGA
PinAI        1933  ACCGGT               A^CCGGT
SexAI        2239  ACCTGGT              A^CC[AT]GGT
SphI           13  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AatII        3087  GACGTC               GACGT^C
             3906  GACGTC              
AccI         3090  GTCTAC               GT^[AC][GT]AC
             3576  GTCTAC              
AcsI         2696  AAATTT               [GA]^AATT[CT]
             2919  AAATTT              
ApoI         2696  AAATTT               [GA]^AATT[CT]
             2919  AAATTT              
AviII          10  TGCGCA               TGC^GCA
             2258  TGCGCA              
BbeI          111  GGCGCC               GGCGC^C
             3313  GGCGCC              
BfaI          458  CTAG                 C^TAG
             2379  CTAG                
BsaI         1041  GAGACC               GGTCTC(1/5)
             2436  GAGACC              
BsmBI         175  GAGACG               CGTCTC(1/5)
             1650  GAGACG              
BsrBI        3012  GAGCGG               CCGCTC(-3/-3)
             3330  GAGCGG              
BstXI         663  CCACCATTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2107  CCAAGGCTGTGG        
FspI           10  TGCGCA               TGC^GCA
             2258  TGCGCA              
HgaI          177  GACGC                GACGC(5/10)
             1652  GACGC               
HindIII      1408  AAGCTT               A^AGCTT
             3720  AAGCTT              
KasI          111  GGCGCC               G^GCGCC
             3313  GGCGCC              
KspI          105  CCGCGG               CCGC^GG
              276  CCGCGG              
MaeI          458  CTAG                 C^TAG
             2379  CTAG                
MseI          850  TTAA                 T^TAA
             1479  TTAA                
NarI          111  GGCGCC               GG^CGCC
             3313  GGCGCC              
NspI           13  GCATGC               [GA]CATG^[CT]
             1784  ACATGT              
SacII         105  CCGCGG               CCGC^GG
              276  CCGCGG              
SfoI          111  GGCGCC               GGC^GCC
             3313  GGCGCC              
SmaI         1945  CCCGGG               CCC^GGG
             3343  CCCGGG              
Tru9I         850  TTAA                 T^TAA
             1479  TTAA                
XmaCI        1945  CCCGGG               C^CCGGG
             3343  CCCGGG              
XmaI         1945  CCCGGG               C^CCGGG
             3343  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        AspHI       
AvaI        AvaII       BalI        BamHI       BanI        
BanII       BbvCI       BbvI        BcnI        BglI        
BglII       BmyI        BpmI        BsaHI       BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BsrI        BssHII      BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DrdI        DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII