##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BbeI BclI BfrI
BglII BlnI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BsrDI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DrdI EagI
EclXI Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflIII 364 ACATGT A^C[GA][CT]GT
Alw44I 828 GTGCAC G^TGCAC
Aor51HI 1309 AGCGCT AGC^GCT
ApaLI 828 GTGCAC G^TGCAC
BamHI 1024 GGATCC G^GATCC
BbsI 549 GTCTTC GAAGAC(2/6)
BbvCI 2222 GCTGAGG CCTCAGC(-5/-2)
BcgI 2559 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 888 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 888 GCTGAGC GC^T[ACGT]AGC
BpuAI 549 GTCTTC GAAGAC(2/6)
BsaBI 1220 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 1046 TCATGA T^CATGA
BspLU11I 364 ACATGT A^CATGT
BssHII 2684 GCGCGC G^CGCGC
BssSI 627 CACGAG CACGAG(-5/-1)
BstEII 1632 GGTCACC G^GT[ACGT]ACC
BstPI 1632 GGTCACC G^GT[ACGT]ACC
BstYI 1024 GGATCC [GA]^GATC[CT]
CelII 888 GCTGAGC GC^T[ACGT]AGC
DraI 2875 TTTAAA TTT^AAA
DraIII 2052 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 810 CTCTTC CTCTTC(1/4)
Eco47III 1309 AGCGCT AGC^GCT
EcoO65I 1632 GGTCACC G^GT[ACGT]ACC
Ksp632I 810 CTCTTC CTCTTC(1/4)
MamI 1220 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1024 GGATCC [GA]^GATC[CT]
MseI 2876 TTAA T^TAA
NaeI 2167 GCCGGC GCC^GGC
NcoI 2044 CCATGG C^CATGG
NgoMIV 2167 GCCGGC G^CCGGC
RcaI 1046 TCATGA T^CATGA
SacI 2501 GAGCTC GAGCT^C
SmlI 408 CTCAAG C^T[CT][GA]AG
Tru9I 2876 TTAA T^TAA
XhoII 1024 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 570 GTCTAC GT^[AC][GT]AC
1595 GTCTAC
AccII 489 CGCG CG^CG
2685 CGCG
AhdI 70 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1074 GACCTGGAGTC
AspEI 70 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1074 GACCTGGAGTC
AspI 233 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
564 GACTCAGTC
BalI 654 TGGCCA TGG^CCA
1442 TGGCCA
BbrPI 1235 CACGTG CAC^GTG
1335 CACGTG
BciVI 35 GTATCC GTATCC(6/5)
101 GTATCC
BfaI 825 CTAG C^TAG
2811 CTAG
BsaAI 1235 CACGTG [CT]AC^GT[GA]
1335 CACGTG
BsaI 2281 GGTCTC GGTCTC(1/5)
2675 GGTCTC
Bsp1407I 2598 TGTACA T^GTACA
2665 TGTACA
BsrFI 634 ACCGGC [GA]^CCGG[CT]
2167 GCCGGC
BsrGI 2598 TGTACA T^GTACA
2665 TGTACA
BstUI 489 CGCG CG^CG
2685 CGCG
Cfr10I 634 ACCGGC [GA]^CCGG[CT]
2167 GCCGGC
DsaI 259 CCACGG C^C[GA][CT]GG
2044 CCATGG
Eam1105I 70 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1074 GACCTGGAGTC
MaeI 825 CTAG C^TAG
2811 CTAG
MaeII 1236 ACGT A^CGT
1336 ACGT
MluNI 654 TGGCCA TGG^CCA
1442 TGGCCA
MscI 654 TGGCCA TGG^CCA
1442 TGGCCA
MslI 1799 CACAGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2590 CACAACAATG
MvnI 489 CGCG CG^CG
2685 CGCG
PflFI 233 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
564 GACTCAGTC
PflMI 2457 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2730 CCAATGGGTGG
PmaCI 1235 CACGTG CAC^GTG
1335 CACGTG
PmlI 1235 CACGTG CAC^GTG
1335 CACGTG
PpuMI 1700 AGGTCCC [GA]G^G[AT]CC[CT]
1766 AGGACCT
SmaI 293 CCCGGG CCC^GGG
1532 CCCGGG
SspBI 2598 TGTACA T^GTACA
2665 TGTACA
StuI 591 AGGCCT AGG^CCT
2563 AGGCCT
TfiI 2787 GATTC G^A[AT]TC
2805 GATTC
Tth111I 233 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
564 GACTCAGTC
Van91I 2457 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2730 CCAATGGGTGG
XcmI 1798 CCACAGGCGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2079 CCAAAACACACCTGG
XmaCI 293 CCCGGG C^CCGGG
1532 CCCGGG
XmaI 293 CCCGGG C^CCGGG
1532 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I