##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Alw44I Aor51HI ApaLI
AscI Asp700I Asp718I AviII AvrII
BbeI BciVI BclI BglII BlnI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoRI EcoRV FbaI FseI FspI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NarI NdeI
NotI NruI NspI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SwaI TfiI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 2328 GTCTAC GT^[AC][GT]AC
AcsI 1921 AAATTC [GA]^AATT[CT]
AflII 2708 CTTAAG C^TTAAG
AflIII 2576 ACACGT A^C[GA][CT]GT
AhdI 3665 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1230 CCTCAGG CC^T[ACGT]AGG
ApoI 1921 AAATTC [GA]^AATT[CT]
AseI 2495 ATTAAT AT^TAAT
AsnI 2495 ATTAAT AT^TAAT
AspEI 3665 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2203 TGGCCA TGG^CCA
BbrPI 2577 CACGTG CAC^GTG
BfrI 2708 CTTAAG C^TTAAG
BsaAI 2577 CACGTG [CT]AC^GT[GA]
BsaWI 3648 ACCGGA [AT]^CCGG[AT]
BsgI 2982 CTGCAC GTGCAG(16/14)
BsmBI 3604 GAGACG CGTCTC(1/5)
BsrBI 3652 GAGCGG CCGCTC(-3/-3)
Bsu36I 1230 CCTCAGG CC^T[ACGT]AGG
DraI 1918 TTTAAA TTT^AAA
Eam1105I 3665 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1230 CCTCAGG CC^T[ACGT]AGG
EcoT22I 3365 ATGCAT ATGCA^T
HaeII 3174 GGCGCT [GA]GCGC^[CT]
HgaI 982 GCGTC GACGC(5/10)
HindIII 1177 AAGCTT A^AGCTT
MluNI 2203 TGGCCA TGG^CCA
MscI 2203 TGGCCA TGG^CCA
NaeI 2538 GCCGGC GCC^GGC
NcoI 2404 CCATGG C^CATGG
NgoMIV 2538 GCCGGC G^CCGGC
NheI 851 GCTAGC G^CTAGC
NsiI 3365 ATGCAT ATGCA^T
PmaCI 2577 CACGTG CAC^GTG
PmlI 2577 CACGTG CAC^GTG
PshBI 2495 ATTAAT AT^TAAT
SacI 2238 GAGCTC GAGCT^C
SexAI 1639 ACCTGGT A^CC[AT]GGT
SgrAI 200 CACCGGCG C[GA]^CCGG[CT]G
SmlI 2708 CTTAAG C^T[CT][GA]AG
SspI 3803 AATATT AAT^ATT
XcmI 1564 CCACAGTTTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 2238 GAGCTC G[AT]GC[AT]^C
3024 GAGCAC
AspI 464 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1638 GACCTGGTC
BamHI 1948 GGATCC G^GATCC
2674 GGATCC
BcgI 2338 CGAGAGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3256 CGATTTCATTGC
BlpI 2663 GCTGAGC GC^T[ACGT]AGC
2953 GCTGAGC
Bpu1102I 2663 GCTGAGC GC^T[ACGT]AGC
2953 GCTGAGC
BsiHKAI 2238 GAGCTC G[AT]GC[AT]^C
3024 GAGCAC
BsrFI 201 ACCGGC [GA]^CCGG[CT]
2538 GCCGGC
BstEII 136 GGTGACC G^GT[ACGT]ACC
2129 GGTGACC
BstPI 136 GGTGACC G^GT[ACGT]ACC
2129 GGTGACC
BstXI 1165 CCACTCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2118 CCAGCCTGCTGG
CelII 2663 GCTGAGC GC^T[ACGT]AGC
2953 GCTGAGC
CfoI 1928 GCGC GCG^C
3175 GCGC
Cfr10I 201 ACCGGC [GA]^CCGG[CT]
2538 GCCGGC
DrdI 1047 GACGGAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2417 GACCCCCCAGTC
EcoNI 2251 CCTCTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3193 CCTTCCCCAGG
EcoO65I 136 GGTGACC G^GT[ACGT]ACC
2129 GGTGACC
HhaI 1928 GCGC GCG^C
3175 GCGC
HinP1I 1928 GCGC G^CGC
3175 GCGC
MslI 677 CACTGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1166 CACTCTGATG
PflFI 464 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1638 GACCTGGTC
PflMI 359 CCACTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2399 CCACCCCATGG
Tth111I 464 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1638 GACCTGGTC
Van91I 359 CCACTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2399 CCACCCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI