##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AcyI AflII AgeI
AhdI AocI ApaI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspMI BssHII BstAPI BstEII BstPI
Bsu36I CelII ClaI CpoI DraIII
DrdI Eam1105I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI Hin1I
KasI MluI MroI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AclI 2888 AACGTT AA^CGTT
Aor51HI 1036 AGCGCT AGC^GCT
BbsI 2515 GAAGAC GAAGAC(2/6)
BbvCI 3154 CCTCAGC CCTCAGC(-5/-2)
BglI 199 GCCGCAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 2515 GAAGAC GAAGAC(2/6)
BsaBI 772 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1679 GGTCTC GGTCTC(1/5)
BsaWI 2451 ACCGGA [AT]^CCGG[AT]
Bsp1407I 2653 TGTACA T^GTACA
BsrFI 2950 GCCGGT [GA]^CCGG[CT]
BsrGI 2653 TGTACA T^GTACA
BssSI 593 CTCGTG CACGAG(-5/-1)
Bst1107I 3042 GTATAC GTA^TAC
BstBI 399 TTCGAA TT^CGAA
BstZ17I 3042 GTATAC GTA^TAC
Cfr10I 2950 GCCGGT [GA]^CCGG[CT]
EagI 99 CGGCCG C^GGCCG
EclXI 99 CGGCCG C^GGCCG
Eco47III 1036 AGCGCT AGC^GCT
Eco52I 99 CGGCCG C^GGCCG
EcoRI 830 GAATTC G^AATTC
HincII 1858 GTTAAC GT[CT]^[GA]AC
HindII 1858 GTTAAC GT[CT]^[GA]AC
HpaI 1858 GTTAAC GTT^AAC
KspI 240 CCGCGG CCGC^GG
MamI 772 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2318 CAATTG C^AATTG
MunI 2318 CAATTG C^AATTG
NheI 487 GCTAGC G^CTAGC
NspV 399 TTCGAA TT^CGAA
PshAI 145 GACGGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2888 AACGTT AA^CGTT
SacI 2267 GAGCTC GAGCT^C
SacII 240 CCGCGG CCGC^GG
SapI 10 GCTCTTC GCTCTTC(1/4)
SfuI 399 TTCGAA TT^CGAA
SmaI 276 CCCGGG CCC^GGG
SspBI 2653 TGTACA T^GTACA
SspI 3016 AATATT AAT^ATT
XmaCI 276 CCCGGG C^CCGGG
XmaI 276 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 990 GGTACC G^GTACC
1374 GGTACC
AccI 2125 GTCTAC GT^[AC][GT]AC
3042 GTATAC
AflIII 1393 ACATGT A^C[GA][CT]GT
2131 ACATGT
Alw44I 68 GTGCAC G^TGCAC
1789 GTGCAC
ApaLI 68 GTGCAC G^TGCAC
1789 GTGCAC
Asp718I 990 GGTACC G^GTACC
1374 GGTACC
BseRI 1449 CTCCTC GAGGAG(10/8)
1769 CTCCTC
BsiEI 99 CGGCCG CG[GA][CT]^CG
1237 CGACCG
BspLU11I 1393 ACATGT A^CATGT
2131 ACATGT
BsrBI 1259 GAGCGG CCGCTC(-3/-3)
1490 GAGCGG
BsrDI 1756 CATTGC GCAATG(2/0)
2745 GCAATG
BstYI 771 GGATCT [GA]^GATC[CT]
2454 GGATCT
HindIII 1945 AAGCTT A^AGCTT
2851 AAGCTT
KpnI 990 GGTACC GGTAC^C
1374 GGTACC
MflI 771 GGATCT [GA]^GATC[CT]
2454 GGATCT
PaeR7I 645 CTCGAG C^TCGAG
2021 CTCGAG
PpuMI 1152 AGGACCC [GA]G^G[AT]CC[CT]
1535 AGGACCC
XcmI 663 CCATCAGCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
757 CCAGCTGTACTTTGG
XhoI 645 CTCGAG C^TCGAG
2021 CTCGAG
XhoII 771 GGATCT [GA]^GATC[CT]
2454 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I