##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AflIII AhdI AocI Aor51HI AscI
AseI AsnI AspEI AviII AvrII
BalI BbeI BbrPI BbsI BbvCI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BseRI BsiEI
BsmI BspDI BspHI BspLU11I BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I FseI FspI
Hin1I HincII HindII HpaI KasI
MamI MluI MluNI MscI NarI
NcoI NdeI NheI NotI NruI
NspV PacI PaeR7I PflMI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 2278 CGCG CG^CG
AccIII 1167 TCCGGA T^CCGGA
AgeI 1415 ACCGGT A^CCGGT
Alw44I 389 GTGCAC G^TGCAC
AlwNI 2804 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1476 GGGCCC GGGCC^C
ApaLI 389 GTGCAC G^TGCAC
BfaI 682 CTAG C^TAG
BlpI 3039 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3039 GCTGAGC GC^T[ACGT]AGC
BsaI 2195 GGTCTC GGTCTC(1/5)
BseAI 1167 TCCGGA T^CCGGA
BsiWI 194 CGTACG C^GTACG
BsmBI 3005 CGTCTC CGTCTC(1/5)
Bsp1407I 830 TGTACA T^GTACA
BspEI 1167 TCCGGA T^CCGGA
BsrBI 2262 GAGCGG CCGCTC(-3/-3)
BsrGI 830 TGTACA T^GTACA
BstUI 2278 CGCG CG^CG
CelII 3039 GCTGAGC GC^T[ACGT]AGC
CfoI 2440 GCGC GCG^C
DraII 1682 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 2287 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 410 TGGCCG [CT]^GGCC[GA]
EarI 2534 GAAGAG CTCTTC(1/4)
EcoO109I 1682 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 329 GATATC GAT^ATC
HaeII 2439 GGCGCT [GA]GCGC^[CT]
HgaI 3036 GACGC GACGC(5/10)
HhaI 2440 GCGC GCG^C
HinP1I 2440 GCGC G^CGC
Ksp632I 2534 GAAGAG CTCTTC(1/4)
KspI 2277 CCGCGG CCGC^GG
MaeI 682 CTAG C^TAG
MfeI 1265 CAATTG C^AATTG
MroI 1167 TCCGGA T^CCGGA
MunI 1265 CAATTG C^AATTG
MvnI 2278 CGCG CG^CG
PinAI 1415 ACCGGT A^CCGGT
SacII 2277 CCGCGG CCGC^GG
SbfI 2946 CCTGCAGG CCTGCA^GG
ScaI 2755 AGTACT AGT^ACT
SphI 2848 GCATGC GCATG^C
Sse8387I 2946 CCTGCAGG CCTGCA^GG
SspBI 830 TGTACA T^GTACA
SspI 972 AATATT AAT^ATT
StuI 138 AGGCCT AGG^CCT
SwaI 3266 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 2076 GGTACC G^GTACC
2505 GGTACC
Asp700I 77 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2363 GAATTGGTTC
Asp718I 2076 GGTACC G^GTACC
2505 GGTACC
AspI 1339 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1564 GACTTTGTC
AvaII 1950 GGACC G^G[AT]CC
2981 GGACC
BamHI 762 GGATCC G^GATCC
791 GGATCC
BciVI 2416 GGATAC GTATCC(6/5)
1518 GTATCC
BclI 62 TGATCA T^GATCA
2639 TGATCA
BsaWI 1167 TCCGGA [AT]^CCGG[AT]
1415 ACCGGT
DraI 1774 TTTAAA TTT^AAA
3267 TTTAAA
EcoRI 2086 GAATTC G^AATTC
2624 GAATTC
EcoT22I 856 ATGCAT ATGCA^T
1446 ATGCAT
FauI 1310 CCCGC CCCGC(4/6)
2276 CCCGC
FbaI 62 TGATCA T^GATCA
2639 TGATCA
KpnI 2076 GGTACC GGTAC^C
2505 GGTACC
NaeI 520 GCCGGC GCC^GGC
2383 GCCGGC
NgoMIV 520 GCCGGC G^CCGGC
2383 GCCGGC
NsiI 856 ATGCAT ATGCA^T
1446 ATGCAT
NspI 1444 ACATGC [GA]CATG^[CT]
2848 GCATGC
PflFI 1339 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1564 GACTTTGTC
Tth111I 1339 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1564 GACTTTGTC
XmnI 77 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2363 GAATTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI BanI BanII
BbvI BcgI BcnI BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr10I
Cfr13I DdeI DpnII DsaI EcoNI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI