##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI Asp718I
AspEI AspI AviII BamHI BbeI
BbrPI BbvCI BcgI BfrI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Bsu36I CfoI Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV FauI FseI FspI HaeII
HhaI HinP1I HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
Tth111I XcmI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1131 GTATAC GT^[AC][GT]AC
AcyI 502 GGCGTC G[GA]^CG[CT]C
ApaI 2208 GGGCCC GGGCC^C
AseI 664 ATTAAT AT^TAAT
AsnI 664 ATTAAT AT^TAAT
AspHI 201 GTGCTC G[AT]GC[AT]^C
AvrII 562 CCTAGG C^CTAGG
BanI 1726 GGTGCC G^G[CT][GA]CC
BciVI 73 GGATAC GTATCC(6/5)
BglI 692 GCCTTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 562 CCTAGG C^CTAGG
BsaHI 502 GGCGTC G[GA]^CG[CT]C
BseRI 2307 GAGGAG GAGGAG(10/8)
BsiHKAI 201 GTGCTC G[AT]GC[AT]^C
BspMI 2016 GCAGGT ACCTGC(4/8)
Bst1107I 1131 GTATAC GTA^TAC
BstXI 492 CCAATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1131 GTATAC GTA^TAC
DraII 2208 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 2466 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 758 CCATGG C^C[GA][CT]GG
EcoO109I 2208 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 3288 GAATTC G^AATTC
EcoT22I 2510 ATGCAT ATGCA^T
HgaI 503 GCGTC GACGC(5/10)
Hin1I 502 GGCGTC G[GA]^CG[CT]C
HincII 1646 GTTGAC GT[CT]^[GA]AC
HindII 1646 GTTGAC GT[CT]^[GA]AC
HindIII 1707 AAGCTT A^AGCTT
NcoI 758 CCATGG C^CATGG
NsiI 2510 ATGCAT ATGCA^T
PaeR7I 2199 CTCGAG C^TCGAG
PshBI 664 ATTAAT AT^TAAT
PstI 1021 CTGCAG CTGCA^G
SmaI 2205 CCCGGG CCC^GGG
SmlI 2199 CTCGAG C^T[CT][GA]AG
StuI 852 AGGCCT AGG^CCT
SwaI 2641 ATTTAAAT ATTT^AAAT
XbaI 515 TCTAGA T^CTAGA
XhoI 2199 CTCGAG C^TCGAG
XmaCI 2205 CCCGGG C^CCGGG
XmaI 2205 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 572 ACATGT A^C[GA][CT]GT
1098 ACATGT
AlwNI 1365 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
1695 CAGGGACTG
Asp700I 401 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1602 GAATTACTTC
AvaI 2199 CTCGAG C^[CT]CG[GA]G
2205 CCCGGG
BalI 53 TGGCCA TGG^CCA
609 TGGCCA
BanII 2202 GAGCCC G[GA]GC[CT]^C
2208 GGGCCC
BbsI 961 GAAGAC GAAGAC(2/6)
2881 GTCTTC
BclI 1525 TGATCA T^GATCA
3365 TGATCA
BlpI 62 GCTCAGC GC^T[ACGT]AGC
229 GCTTAGC
Bpu1102I 62 GCTCAGC GC^T[ACGT]AGC
229 GCTTAGC
BpuAI 961 GAAGAC GAAGAC(2/6)
2881 GTCTTC
BsgI 1714 CTGCAC GTGCAG(16/14)
1905 GTGCAG
BsoBI 2199 CTCGAG C^[CT]CG[GA]G
2205 CCCGGG
BspLU11I 572 ACATGT A^CATGT
1098 ACATGT
BstF5I 1866 CATCC GGATG(2/0)
2146 GGATG
Cac8I 1066 GCTTGC GC[ACGT]^[ACGT]GC
1153 GCAAGC
CelII 62 GCTCAGC GC^T[ACGT]AGC
229 GCTTAGC
EaeI 53 TGGCCA [CT]^GGCC[GA]
609 TGGCCA
EcoT14I 562 CCTAGG C^C[AT][AT]GG
758 CCATGG
FbaI 1525 TGATCA T^GATCA
3365 TGATCA
FokI 1866 CATCC GGATG(9/13)
2146 GGATG
HapII 1167 CCGG C^CGG
2206 CCGG
HpaII 1167 CCGG C^CGG
2206 CCGG
MluNI 53 TGGCCA TGG^CCA
609 TGGCCA
MscI 53 TGGCCA TGG^CCA
609 TGGCCA
MslI 2640 CATTTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2889 CATCACTGTG
MspA1I 108 CAGCTG C[AC]G^C[GT]G
1206 CAGCTG
MspI 1167 CCGG C^CGG
2206 CCGG
NdeI 1521 CATATG CA^TATG
2942 CATATG
PflMI 2093 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2288 CCAACTGGTGG
PvuII 108 CAGCTG CAG^CTG
1206 CAGCTG
SexAI 196 ACCTGGT A^CC[AT]GGT
2601 ACCTGGT
SspI 1422 AATATT AAT^ATT
3353 AATATT
StyI 562 CCTAGG C^C[AT][AT]GG
758 CCATGG
TaqI 172 TCGA T^CGA
2200 TCGA
TthHB8I 172 TCGA T^CGA
2200 TCGA
Van91I 2093 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2288 CCAACTGGTGG
XmnI 401 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1602 GAATTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BcnI BfaI BglII
BmyI BpmI BsaI BsaJI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstNI BstYI Cfr13I
DdeI DpnII DraI EarI EcoRII
Fnu4HI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII