##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BalI
BanI BbeI BbvCI BcgI BclI
BfrI BlnI BsaHI BsaI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BspMI BsrDI BsrFI
BssHII BssSI BstEII BstPI BstXI
Bsu36I Cfr10I ClaI CpoI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI Hin1I HindIII HpaI
KasI KpnI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI SphI Sse8387I SwaI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 401 GTATAC GT^[AC][GT]AC
AspI 1943 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 867 CACGTG CAC^GTG
BciVI 687 GTATCC GTATCC(6/5)
BsaAI 867 CACGTG [CT]AC^GT[GA]
BsaBI 2597 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1290 TCCGGT [AT]^CCGG[AT]
Bsp1407I 1779 TGTACA T^GTACA
BspHI 1468 TCATGA T^CATGA
BsrGI 1779 TGTACA T^GTACA
Bst1107I 401 GTATAC GTA^TAC
BstBI 3282 TTCGAA TT^CGAA
BstZ17I 401 GTATAC GTA^TAC
DraIII 932 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2955 GACTGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3271 GAATTC G^AATTC
HgaI 323 GACGC GACGC(5/10)
HincII 3054 GTCAAC GT[CT]^[GA]AC
HindII 3054 GTCAAC GT[CT]^[GA]AC
KspI 243 CCGCGG CCGC^GG
MamI 2597 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1594 CCATGG C^CATGG
NheI 724 GCTAGC G^CTAGC
NspI 2865 GCATGT [GA]CATG^[CT]
NspV 3282 TTCGAA TT^CGAA
PaeR7I 453 CTCGAG C^TCGAG
PflFI 1943 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 828 CCATCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 867 CACGTG CAC^GTG
PmlI 867 CACGTG CAC^GTG
RcaI 1468 TCATGA T^CATGA
SacI 1365 GAGCTC GAGCT^C
SacII 243 CCGCGG CCGC^GG
SfuI 3282 TTCGAA TT^CGAA
SmaI 175 CCCGGG CCC^GGG
SpeI 2204 ACTAGT A^CTAGT
SspBI 1779 TGTACA T^GTACA
StuI 975 AGGCCT AGG^CCT
Tth111I 1943 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 828 CCATCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 713 TCTAGA T^CTAGA
XhoI 453 CTCGAG C^TCGAG
XmaCI 175 CCCGGG C^CCGGG
XmaI 175 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 169 CGCG CG^CG
244 CGCG
AcsI 1831 AAATTT [GA]^AATT[CT]
3271 GAATTC
AhdI 192 GACCCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3222 GACAGCAAGTC
ApaI 179 GGGCCC GGGCC^C
331 GGGCCC
ApoI 1831 AAATTT [GA]^AATT[CT]
3271 GAATTC
AspEI 192 GACCCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3222 GACAGCAAGTC
AspHI 1310 GAGCAC G[AT]GC[AT]^C
1365 GAGCTC
AvaI 175 CCCGGG C^[CT]CG[GA]G
453 CTCGAG
BamHI 781 GGATCC G^GATCC
1228 GGATCC
BbsI 998 GAAGAC GAAGAC(2/6)
2360 GTCTTC
BglI 242 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2611 GCCCCCGTGGC
BglII 2066 AGATCT A^GATCT
3341 AGATCT
BlpI 2426 GCTTAGC GC^T[ACGT]AGC
2817 GCTTAGC
Bpu1102I 2426 GCTTAGC GC^T[ACGT]AGC
2817 GCTTAGC
BpuAI 998 GAAGAC GAAGAC(2/6)
2360 GTCTTC
BsgI 3096 CTGCAC GTGCAG(16/14)
2472 GTGCAG
BsiHKAI 1310 GAGCAC G[AT]GC[AT]^C
1365 GAGCTC
BsoBI 175 CCCGGG C^[CT]CG[GA]G
453 CTCGAG
BstAPI 18 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
579 GCACAAAATGC
BstUI 169 CGCG CG^CG
244 CGCG
CelII 2426 GCTTAGC GC^T[ACGT]AGC
2817 GCTTAGC
Eam1105I 192 GACCCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3222 GACAGCAAGTC
HaeII 250 GGCGCT [GA]GCGC^[CT]
295 AGCGCC
MaeII 868 ACGT A^CGT
1251 ACGT
MvnI 169 CGCG CG^CG
244 CGCG
PstI 2773 CTGCAG CTGCA^G
2846 CTGCAG
PvuII 2950 CAGCTG CAG^CTG
3006 CAGCTG
SspI 2158 AATATT AAT^ATT
2757 AATATT
XcmI 673 CCAGCAGGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2606 CCATAGCCCCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII