##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AspEI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BciVI BclI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BseAI BsiEI BsiWI BsoBI BspDI
BspEI BspLU11I BsrBI BssHII BstBI
BstEII BstPI BstUI BstYI CelII
ClaI CpoI DraII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I FbaI FseI FspI HaeII
Hin1I HincII HindII HpaI KasI
KspI MflI MluI MroI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI XcmI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 459 GGTACC G^GTACC
AclI 1624 AACGTT AA^CGTT
AflII 908 CTTAAG C^TTAAG
Asp700I 2123 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 459 GGTACC G^GTACC
AspI 2680 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 459 GGTACC G^G[CT][GA]CC
BbvCI 61 CCTCAGC CCTCAGC(-5/-2)
BfrI 908 CTTAAG C^TTAAG
BsaBI 3807 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 706 TCCGGT [AT]^CCGG[AT]
BseRI 765 GAGGAG GAGGAG(10/8)
BsmI 1050 GAATGC GAATGC(1/-1)
Bsp1407I 536 TGTACA T^GTACA
BsrFI 3779 GCCGGT [GA]^CCGG[CT]
BsrGI 536 TGTACA T^GTACA
BssSI 2902 CTCGTG CACGAG(-5/-1)
Bst1107I 2170 GTATAC GTA^TAC
BstAPI 3208 GCAATTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2170 GTATAC GTA^TAC
CfoI 1252 GCGC GCG^C
Cfr10I 3779 GCCGGT [GA]^CCGG[CT]
DrdI 1943 GACTATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 3372 GATATC GAT^ATC
EcoT22I 2291 ATGCAT ATGCA^T
FauI 2882 GCGGG CCCGC(4/6)
HgaI 507 GACGC GACGC(5/10)
HhaI 1252 GCGC GCG^C
HinP1I 1252 GCGC G^CGC
HindIII 1235 AAGCTT A^AGCTT
KpnI 459 GGTACC GGTAC^C
MamI 3807 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1191 CAATTG C^AATTG
MunI 1191 CAATTG C^AATTG
NsiI 2291 ATGCAT ATGCA^T
PflFI 2680 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1624 AACGTT AA^CGTT
PstI 3756 CTGCAG CTGCA^G
ScaI 737 AGTACT AGT^ACT
SmlI 908 CTTAAG C^T[CT][GA]AG
SphI 2293 GCATGC GCATG^C
SspBI 536 TGTACA T^GTACA
SspI 2570 AATATT AAT^ATT
Tth111I 2680 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 761 TCTAGA T^CTAGA
XmnI 2123 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 2125 ACGTGT A^C[GA][CT]GT
3870 ACACGT
AlwI 2089 GATCC GGATC(4/5)
2907 GATCC
AocI 490 CCTAAGG CC^T[ACGT]AGG
2093 CCTCAGG
AspHI 692 GAGCAC G[AT]GC[AT]^C
3162 GTGCTC
BbsI 830 GAAGAC GAAGAC(2/6)
3467 GAAGAC
BcgI 2762 CGATTCTTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
387 GCAGTGTTCTCG
BmyI 692 GAGCAC G[AGT]GC[ACT]^C
3162 GTGCTC
BpuAI 830 GAAGAC GAAGAC(2/6)
3467 GAAGAC
BsiHKAI 692 GAGCAC G[AT]GC[AT]^C
3162 GTGCTC
BsmBI 1682 CGTCTC CGTCTC(1/5)
1803 GAGACG
Bsp1286I 692 GAGCAC G[AGT]GC[ACT]^C
3162 GTGCTC
Bsu36I 490 CCTAAGG CC^T[ACGT]AGG
2093 CCTCAGG
DsaI 2358 CCATGG C^C[GA][CT]GG
2399 CCATGG
Eco81I 490 CCTAAGG CC^T[ACGT]AGG
2093 CCTCAGG
NcoI 2358 CCATGG C^CATGG
2399 CCATGG
NdeI 2052 CATATG CA^TATG
2165 CATATG
PflMI 2018 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3928 CCATTTAGTGG
SfcI 3098 CTACAG C^T[GA][CT]AG
3756 CTGCAG
Van91I 2018 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3928 CCATTTAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI AseI AsnI AvaII BalI
BbvI BfaI BpmI BsaI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BspHI BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII DraI DraIII EaeI
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I