##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AviII AvrII BamHI BbeI
BbrPI BclI BglII BlnI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrFI BssSI
BstAPI BstBI BstEII BstPI Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I StuI
SwaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccI 2301 GTATAC GT^[AC][GT]AC
AccII 2725 CGCG CG^CG
AclI 2545 AACGTT AA^CGTT
AflII 790 CTTAAG C^TTAAG
AhdI 83 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2122 CCTGAGG CC^T[ACGT]AGG
AseI 770 ATTAAT AT^TAAT
AsnI 770 ATTAAT AT^TAAT
AspEI 83 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 476 GAGCTC G[AT]GC[AT]^C
AspI 122 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 313 CTCGGG C^[CT]CG[GA]G
BanI 2898 GGTGCC G^G[CT][GA]CC
BbvCI 2694 CCTCAGC CCTCAGC(-5/-2)
BcgI 1291 GCAGCTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1888 GGATAC GTATCC(6/5)
BcnI 2830 CCGGG CC^[GC]GG
BfrI 790 CTTAAG C^TTAAG
BglI 1856 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1935 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1935 GCTGAGC GC^T[ACGT]AGC
BsaAI 1111 TACGTA [CT]AC^GT[GA]
BsgI 879 CTGCAC GTGCAG(16/14)
BsiHKAI 476 GAGCTC G[AT]GC[AT]^C
BsmBI 2766 GAGACG CGTCTC(1/5)
BsoBI 313 CTCGGG C^[CT]CG[GA]G
BspHI 352 TCATGA T^CATGA
BspLU11I 2108 ACATGT A^CATGT
BspMI 2238 GCAGGT ACCTGC(4/8)
BsrBI 1180 CCGCTC CCGCTC(-3/-3)
BsrDI 3051 CATTGC GCAATG(2/0)
BssHII 2724 GCGCGC G^CGCGC
Bst1107I 2301 GTATAC GTA^TAC
BstUI 2725 CGCG CG^CG
BstZ17I 2301 GTATAC GTA^TAC
Bsu36I 2122 CCTGAGG CC^T[ACGT]AGG
CelII 1935 GCTGAGC GC^T[ACGT]AGC
Eam1105I 83 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2122 CCTGAGG CC^T[ACGT]AGG
EcoNI 1448 CCTGTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 151 GAATTC G^AATTC
EcoT22I 2311 ATGCAT ATGCA^T
FauI 1179 CCCGC CCCGC(4/6)
HapII 2830 CCGG C^CGG
HgaI 1909 GACGC GACGC(5/10)
HpaII 2830 CCGG C^CGG
MspI 2830 CCGG C^CGG
MvnI 2725 CGCG CG^CG
NciI 2830 CCGGG CC^[GC]GG
NdeI 1722 CATATG CA^TATG
NsiI 2311 ATGCAT ATGCA^T
NspI 2108 ACATGT [GA]CATG^[CT]
PflFI 122 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1525 CCATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1150 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 770 ATTAAT AT^TAAT
Psp1406I 2545 AACGTT AA^CGTT
RcaI 352 TCATGA T^CATGA
SacI 476 GAGCTC GAGCT^C
SnaBI 1111 TACGTA TAC^GTA
Tth111I 122 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1525 CCATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3140 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 1180 CCGC CCGC(-3/-1)
2506 GCGG
AflIII 2108 ACATGT A^C[GA][CT]GT
2854 ACGTGT
AlwNI 32 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1011 CAGGAACTG
AvaII 418 GGACC G^G[AT]CC
1459 GGACC
BanII 476 GAGCTC G[GA]GC[CT]^C
1815 GGGCTC
BmyI 476 GAGCTC G[AGT]GC[ACT]^C
1815 GGGCTC
BsmI 44 GAATGC GAATGC(1/-1)
1080 GAATGC
Bsp1286I 476 GAGCTC G[AGT]GC[ACT]^C
1815 GGGCTC
BstYI 682 AGATCC [GA]^GATC[CT]
2596 GGATCT
CfoI 2724 GCGC GCG^C
2726 GCGC
DraI 2229 TTTAAA TTT^AAA
3165 TTTAAA
DsaI 759 CCATGG C^C[GA][CT]GG
1860 CCATGG
EcoT14I 759 CCATGG C^C[AT][AT]GG
1860 CCATGG
HhaI 2724 GCGC GCG^C
2726 GCGC
HinP1I 2724 GCGC G^CGC
2726 GCGC
HphI 282 TCACC GGTGA(8/7)
3020 TCACC
MflI 682 AGATCC [GA]^GATC[CT]
2596 GGATCT
NcoI 759 CCATGG C^CATGG
1860 CCATGG
PpuMI 417 AGGACCC [GA]G^G[AT]CC[CT]
1458 GGGACCT
PstI 675 CTGCAG CTGCA^G
2656 CTGCAG
SapI 473 GAAGAGC GCTCTTC(1/4)
1125 GAAGAGC
ScaI 131 AGTACT AGT^ACT
2394 AGTACT
SfaNI 764 GATGC GCATC(5/9)
279 GCATC
SmlI 790 CTTAAG C^T[CT][GA]AG
1049 CTCAAG
StyI 759 CCATGG C^C[AT][AT]GG
1860 CCATGG
Tsp45I 2682 GTGAC ^GT[GC]AC
3019 GTCAC
XhoII 682 AGATCC [GA]^GATC[CT]
2596 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BalI BbsI BbvI BfaI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI SspBI SspI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XmnI