##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AcyI AflIII AgeI AhdI ApoI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbeI BclI BlnI
BsaBI BsaHI BsmBI Bsp1407I BspDI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I ClaI CpoI
DraI DrdI Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MunI MvnI
NarI NdeI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SwaI
Tsp509I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AflII 3190 CTTAAG C^TTAAG
Alw44I 1138 GTGCAC G^TGCAC
Aor51HI 2119 AGCGCT AGC^GCT
ApaLI 1138 GTGCAC G^TGCAC
Asp700I 1443 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2678 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2965 GGATCC G^GATCC
BbrPI 1917 CACGTG CAC^GTG
BbsI 3142 GTCTTC GAAGAC(2/6)
BbvCI 533 GCTGAGG CCTCAGC(-5/-2)
BcgI 1652 GCAGTTGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2664 GTATCC GTATCC(6/5)
BfrI 3190 CTTAAG C^TTAAG
BglII 1592 AGATCT A^GATCT
BpuAI 3142 GTCTTC GAAGAC(2/6)
BsaAI 1917 CACGTG [CT]AC^GT[GA]
BsiEI 1055 CGGCCG CG[GA][CT]^CG
BsiWI 1430 CGTACG C^GTACG
BsmI 1443 GAATGC GAATGC(1/-1)
BspHI 1030 TCATGA T^CATGA
EagI 1055 CGGCCG C^GGCCG
EclXI 1055 CGGCCG C^GGCCG
Eco47III 2119 AGCGCT AGC^GCT
Eco52I 1055 CGGCCG C^GGCCG
HgaI 1658 GCGTC GACGC(5/10)
HincII 1718 GTTGAC GT[CT]^[GA]AC
HindII 1718 GTTGAC GT[CT]^[GA]AC
MaeII 1918 ACGT A^CGT
NaeI 1109 GCCGGC GCC^GGC
NgoMIV 1109 GCCGGC G^CCGGC
NheI 2325 GCTAGC G^CTAGC
PflFI 2678 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1917 CACGTG CAC^GTG
PmlI 1917 CACGTG CAC^GTG
PshAI 1721 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1030 TCATGA T^CATGA
SbfI 2357 CCTGCAGG CCTGCA^GG
SexAI 3235 ACCTGGT A^CC[AT]GGT
SfiI 2385 GGCCTGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 697 CCCGGG CCC^GGG
Sse8387I 2357 CCTGCAGG CCTGCA^GG
SspI 3249 AATATT AAT^ATT
StuI 1860 AGGCCT AGG^CCT
Tth111I 2678 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 697 CCCGGG C^CCGGG
XmaI 697 CCCGGG C^CCGGG
XmnI 1443 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1792 GTCTAC GT^[AC][GT]AC
3087 GTAGAC
AccIII 1095 TCCGGA T^CCGGA
3004 TCCGGA
BsaI 177 GAGACC GGTCTC(1/5)
3105 GGTCTC
BsaWI 1095 TCCGGA [AT]^CCGG[AT]
3004 TCCGGA
BseAI 1095 TCCGGA T^CCGGA
3004 TCCGGA
BspEI 1095 TCCGGA T^CCGGA
3004 TCCGGA
BspMI 568 ACCTGC ACCTGC(4/8)
2655 ACCTGC
BstEII 2031 GGTGACC G^GT[ACGT]ACC
2552 GGTCACC
BstPI 2031 GGTGACC G^GT[ACGT]ACC
2552 GGTCACC
DraIII 1132 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2920 CACTCCGTG
EcoNI 1472 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2288 CCTGTTGAAGG
EcoO65I 2031 GGTGACC G^GT[ACGT]ACC
2552 GGTCACC
HaeII 2119 AGCGCT [GA]GCGC^[CT]
3023 GGCGCT
MroI 1095 TCCGGA T^CCGGA
3004 TCCGGA
MseI 907 TTAA T^TAA
3191 TTAA
MslI 595 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1730 CACCAATGTG
SapI 2156 GCTCTTC GCTCTTC(1/4)
2879 GCTCTTC
ScaI 685 AGTACT AGT^ACT
822 AGTACT
TaqI 13 TCGA T^CGA
730 TCGA
Tru9I 907 TTAA T^TAA
3191 TTAA
TthHB8I 13 TCGA T^CGA
730 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI TseI Tsp45I TspRI Van91I
XcmI XhoII