##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AvaI AviII AvrII
BbrPI BglI BglII BlnI BlpI
Bpu1102I BsaAI BseAI BsiEI BsiWI
BsmI BsoBI BspDI BspEI BsrBI
BstBI BstEII BstPI Bsu36I CelII
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FseI FspI HindIII HpaI
KpnI KspI MluI MroI NheI
NotI NruI NspV PacI PaeR7I
PflMI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AcyI 1506 GGCGCC G[GA]^CG[CT]C
AgeI 2860 ACCGGT A^CCGGT
Alw44I 1485 GTGCAC G^TGCAC
AlwNI 1743 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1485 GTGCAC G^TGCAC
Asp700I 2216 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3146 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1004 TGGCCA TGG^CCA
BbeI 1506 GGCGCC GGCGC^C
BbvCI 2444 CCTCAGC CCTCAGC(-5/-2)
BciVI 3372 GTATCC GTATCC(6/5)
BclI 560 TGATCA T^GATCA
BsaBI 2808 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1506 GGCGCC G[GA]^CG[CT]C
BsaI 3508 GAGACC GGTCTC(1/5)
BsaWI 2860 ACCGGT [AT]^CCGG[AT]
BseRI 2236 GAGGAG GAGGAG(10/8)
BsmBI 1415 CGTCTC CGTCTC(1/5)
BspLU11I 3160 ACATGT A^CATGT
BsrDI 1934 CATTGC GCAATG(2/0)
BssHII 23 GCGCGC G^CGCGC
BssSI 2025 CACGAG CACGAG(-5/-1)
Bst1107I 3601 GTATAC GTA^TAC
BstAPI 1774 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3601 GTATAC GTA^TAC
EaeI 1004 TGGCCA [CT]^GGCC[GA]
EcoRI 592 GAATTC G^AATTC
EcoT22I 2715 ATGCAT ATGCA^T
FauI 101 GCGGG CCCGC(4/6)
FbaI 560 TGATCA T^GATCA
HaeII 1506 GGCGCC [GA]GCGC^[CT]
Hin1I 1506 GGCGCC G[GA]^CG[CT]C
KasI 1506 GGCGCC G^GCGCC
MamI 2808 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2817 CAATTG C^AATTG
MluNI 1004 TGGCCA TGG^CCA
MscI 1004 TGGCCA TGG^CCA
MunI 2817 CAATTG C^AATTG
NaeI 123 GCCGGC GCC^GGC
NarI 1506 GGCGCC GG^CGCC
NgoMIV 123 GCCGGC G^CCGGC
NsiI 2715 ATGCAT ATGCA^T
PflFI 3146 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2860 ACCGGT A^CCGGT
PpuMI 2455 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 105 GAGCTC GAGCT^C
SfoI 1506 GGCGCC GGC^GCC
SphI 1066 GCATGC GCATG^C
Tth111I 3146 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2141 TCTAGA T^CTAGA
XcmI 3231 CCACAGTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2216 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflII 572 CTTAAG C^TTAAG
2699 CTTAAG
ApaI 1141 GGGCCC GGGCC^C
1662 GGGCCC
BamHI 644 GGATCC G^GATCC
3057 GGATCC
BbsI 1852 GTCTTC GAAGAC(2/6)
3122 GTCTTC
BfrI 572 CTTAAG C^TTAAG
2699 CTTAAG
BpuAI 1852 GTCTTC GAAGAC(2/6)
3122 GTCTTC
BsgI 1278 CTGCAC GTGCAG(16/14)
3179 GTGCAG
BsrFI 123 GCCGGC [GA]^CCGG[CT]
2860 ACCGGT
BstXI 3269 CCAGCACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3544 CCAGACAGATGG
Cfr10I 123 GCCGGC [GA]^CCGG[CT]
2860 ACCGGT
DraIII 1281 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
3173 CACCATGTG
EarI 296 GAAGAG CTCTTC(1/4)
549 GAAGAG
EcoNI 1361 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1666 CCTCCAGCAGG
HgaI 20 GACGC GACGC(5/10)
78 GACGC
Ksp632I 296 GAAGAG CTCTTC(1/4)
549 GAAGAG
MspA1I 1743 CAGCTG C[AC]G^C[GT]G
3182 CAGCTG
NcoI 1684 CCATGG C^CATGG
2460 CCATGG
NdeI 1000 CATATG CA^TATG
3015 CATATG
PshAI 504 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2859 GACCGGTGTC
PvuII 1743 CAGCTG CAG^CTG
3182 CAGCTG
StuI 2615 AGGCCT AGG^CCT
2954 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AspHI
AvaII BanI BanII BbvI BcgI
BcnI BfaI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspHI BspMI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII