##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I ApaLI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BciVI BglI BlnI
BlpI Bpu1102I BsaHI BsaI BseAI
BsiWI BsmBI BsoBI BspDI BspEI
BsrFI BssSI Bst1107I BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DrdI EaeI EagI Eam1105I EclXI
Eco52I EcoO65I FseI FspI Hin1I
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacII
SalI SapI SbfI SfiI SfoI
SgrAI SmaI SphI Sse8387I Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1530 GTCTAC GT^[AC][GT]AC
AclI 5199 AACGTT AA^CGTT
AflII 3524 CTTAAG C^TTAAG
AflIII 3261 ACATGT A^C[GA][CT]GT
AocI 1700 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1028 AGCGCT AGC^GCT
ApaI 98 GGGCCC GGGCC^C
AscI 122 GGCGCGCC GG^CGCGCC
BbvCI 3105 GCTGAGG CCTCAGC(-5/-2)
BcgI 3487 CGATTTAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3524 CTTAAG C^TTAAG
BsaWI 3062 TCCGGT [AT]^CCGG[AT]
BsiEI 57 CGACCG CG[GA][CT]^CG
Bsp1407I 4738 TGTACA T^GTACA
BspLU11I 3261 ACATGT A^CATGT
BsrDI 740 CATTGC GCAATG(2/0)
BsrGI 4738 TGTACA T^GTACA
BssHII 123 GCGCGC G^CGCGC
BstBI 3811 TTCGAA TT^CGAA
BstXI 1388 CCAAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1700 CCTCAGG CC^T[ACGT]AGG
DsaI 1643 CCATGG C^C[GA][CT]GG
EarI 3091 GAAGAG CTCTTC(1/4)
Eco47III 1028 AGCGCT AGC^GCT
Eco81I 1700 CCTCAGG CC^T[ACGT]AGG
EcoRI 2090 GAATTC G^AATTC
EcoRV 1603 GATATC GAT^ATC
HpaI 3586 GTTAAC GTT^AAC
Ksp632I 3091 GAAGAG CTCTTC(1/4)
NcoI 1643 CCATGG C^CATGG
NdeI 5305 CATATG CA^TATG
NheI 4259 GCTAGC G^CTAGC
NspV 3811 TTCGAA TT^CGAA
PflMI 3909 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 5199 AACGTT AA^CGTT
SexAI 3747 ACCTGGT A^CC[AT]GGT
SfuI 3811 TTCGAA TT^CGAA
SnaBI 4455 TACGTA TAC^GTA
SpeI 4033 ACTAGT A^CTAGT
SspBI 4738 TGTACA T^GTACA
StuI 1176 AGGCCT AGG^CCT
SwaI 2102 ATTTAAAT ATTT^AAAT
Van91I 3909 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BamHI 396 GGATCC G^GATCC
3174 GGATCC
BclI 330 TGATCA T^GATCA
3372 TGATCA
BglII 2682 AGATCT A^GATCT
3306 AGATCT
BsaAI 818 TACGTG [CT]AC^GT[GA]
4455 TACGTA
BsaBI 2428 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2481 GATGACCATC
BsrBI 705 CCGCTC CCGCTC(-3/-3)
2395 GAGCGG
DraIII 184 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
2764 CACCAAGTG
FbaI 330 TGATCA T^GATCA
3372 TGATCA
HaeII 1028 AGCGCT [GA]GCGC^[CT]
3362 GGCGCT
HgaI 84 GACGC GACGC(5/10)
3353 GCGTC
HincII 2703 GTCAAC GT[CT]^[GA]AC
3586 GTTAAC
HindII 2703 GTCAAC GT[CT]^[GA]AC
3586 GTTAAC
HindIII 1222 AAGCTT A^AGCTT
4443 AAGCTT
MamI 2428 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2481 GATGACCATC
PleI 2532 GACTC GAGTC(4/5)
5062 GACTC
ScaI 601 AGTACT AGT^ACT
4075 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PpuMI
PshBI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI