##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AccI AccIII AgeI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspI AvaII AviII AvrII BbrPI
BbvCI BcgI BlnI BlpI Bpu1102I
BsaAI BsaI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspMI BsrFI
BsrGI BssHII Bst1107I BstAPI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraII DrdI EagI EclXI
Eco47III Eco52I EcoO109I EcoO65I FseI
FspI HpaI KpnI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PvuI PvuII RsrII
SalI SbfI SfiI SgrAI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 1679 GACGTC GACGT^C
AclI 1757 AACGTT AA^CGTT
AflII 410 CTTAAG C^TTAAG
AhdI 2967 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 275 CCTGAGG CC^T[ACGT]AGG
AseI 2209 ATTAAT AT^TAAT
AsnI 2209 ATTAAT AT^TAAT
AspEI 2967 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2382 TGGCCA TGG^CCA
BbeI 240 GGCGCC GGCGC^C
BclI 3319 TGATCA T^GATCA
BfrI 410 CTTAAG C^TTAAG
BglI 3294 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1946 AGATCT A^GATCT
BsaBI 3314 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2628 ACCGGA [AT]^CCGG[AT]
BsmBI 113 GAGACG CGTCTC(1/5)
BspHI 4256 TCATGA T^CATGA
BsrBI 1718 GAGCGG CCGCTC(-3/-3)
BsrDI 4018 CATTGC GCAATG(2/0)
BssSI 4060 CTCGTG CACGAG(-5/-1)
BstBI 3838 TTCGAA TT^CGAA
BstXI 1850 CCAGAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 275 CCTGAGG CC^T[ACGT]AGG
DraI 4343 TTTAAA TTT^AAA
DraIII 71 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2967 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 275 CCTGAGG CC^T[ACGT]AGG
EcoNI 2650 CCTTGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4252 GAATTC G^AATTC
EcoT22I 2452 ATGCAT ATGCA^T
FbaI 3319 TGATCA T^GATCA
HaeII 240 GGCGCC [GA]GCGC^[CT]
HincII 2624 GTCAAC GT[CT]^[GA]AC
HindII 2624 GTCAAC GT[CT]^[GA]AC
KasI 240 GGCGCC G^GCGCC
KspI 252 CCGCGG CCGC^GG
MamI 3314 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2382 TGGCCA TGG^CCA
MscI 2382 TGGCCA TGG^CCA
MslI 1700 CACAGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 240 GGCGCC GG^CGCC
NdeI 3988 CATATG CA^TATG
NsiI 2452 ATGCAT ATGCA^T
NspV 3838 TTCGAA TT^CGAA
PshBI 2209 ATTAAT AT^TAAT
Psp1406I 1757 AACGTT AA^CGTT
RcaI 4256 TCATGA T^CATGA
SacII 252 CCGCGG CCGC^GG
SfoI 240 GGCGCC GGC^GCC
SfuI 3838 TTCGAA TT^CGAA
SmaI 149 CCCGGG CCC^GGG
SphI 3591 GCATGC GCATG^C
XmaCI 149 CCCGGG C^CCGGG
XmaI 149 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 240 GGCGCC G[GA]^CG[CT]C
1679 GACGTC
AlwNI 1434 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2001 CAGATACTG
AvaI 134 CCCGAG C^[CT]CG[GA]G
149 CCCGGG
BamHI 377 GGATCC G^GATCC
3248 GGATCC
BanI 240 GGCGCC G^G[CT][GA]CC
2477 GGCACC
BbsI 1452 GTCTTC GAAGAC(2/6)
2236 GTCTTC
BciVI 261 GGATAC GTATCC(6/5)
2321 GTATCC
BpuAI 1452 GTCTTC GAAGAC(2/6)
2236 GTCTTC
BsaHI 240 GGCGCC G[GA]^CG[CT]C
1679 GACGTC
BsgI 1100 CTGCAC GTGCAG(16/14)
77 GTGCAG
BsoBI 134 CCCGAG C^[CT]CG[GA]G
149 CCCGGG
Cfr13I 272 GGGCC G^G[ACGT]CC
301 GGCCC
EaeI 246 TGGCCG [CT]^GGCC[GA]
2382 TGGCCA
FauI 166 GCGGG CCCGC(4/6)
254 GCGGG
Hin1I 240 GGCGCC G[GA]^CG[CT]C
1679 GACGTC
HindIII 1547 AAGCTT A^AGCTT
2400 AAGCTT
MspA1I 252 CCGCGG C[AC]G^C[GT]G
2472 CCGCTG
NcoI 533 CCATGG C^CATGG
2544 CCATGG
PleI 1318 GACTC GAGTC(4/5)
2078 GAGTC
PstI 90 CTGCAG CTGCA^G
4023 CTGCAG
SacI 10 GAGCTC GAGCT^C
1188 GAGCTC
SapI 170 GCTCTTC GCTCTTC(1/4)
355 GCTCTTC
Sau96I 272 GGGCC G^G[ACGT]CC
301 GGCCC
ScaI 822 AGTACT AGT^ACT
1325 AGTACT
SexAI 3073 ACCTGGT A^CC[AT]GGT
3157 ACCTGGT
SmlI 410 CTTAAG C^T[CT][GA]AG
2341 CTCAAG
TaqI 3196 TCGA T^CGA
3839 TCGA
TthHB8I 3196 TCGA T^CGA
3839 TCGA
XbaI 1024 TCTAGA T^CTAGA
2429 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspLU11I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI DdeI
DpnII DsaI EarI EcoRII EcoRV
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
RsaI Sau3AI ScrFI SfaNI SfcI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII
XmnI