##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AocI ApaI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsiWI
Bsp1407I BspDI BspHI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoT22I FbaI FseI
FspI HpaI KpnI KspI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PpuMI PshAI PshBI Psp1406I RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccIII 1754 TCCGGA T^CCGGA
AcyI 88 GGCGCC G[GA]^CG[CT]C
Alw44I 2437 GTGCAC G^TGCAC
AlwNI 437 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2437 GTGCAC G^TGCAC
AspHI 2437 GTGCAC G[AT]GC[AT]^C
AspI 2555 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 242 CCCGGG C^[CT]CG[GA]G
BalI 2376 TGGCCA TGG^CCA
BamHI 1433 GGATCC G^GATCC
BanII 2489 GAGCCC G[GA]GC[CT]^C
BbeI 88 GGCGCC GGCGC^C
BbrPI 1524 CACGTG CAC^GTG
BbvCI 2500 GCTGAGG CCTCAGC(-5/-2)
BfaI 1974 CTAG C^TAG
BsaAI 1524 CACGTG [CT]AC^GT[GA]
BsaHI 88 GGCGCC G[GA]^CG[CT]C
BsaI 2366 GAGACC GGTCTC(1/5)
BsaWI 1754 TCCGGA [AT]^CCGG[AT]
BseAI 1754 TCCGGA T^CCGGA
BsiEI 35 CGATCG CG[GA][CT]^CG
BsiHKAI 2437 GTGCAC G[AT]GC[AT]^C
BsmBI 39 CGTCTC CGTCTC(1/5)
BsoBI 242 CCCGGG C^[CT]CG[GA]G
BspEI 1754 TCCGGA T^CCGGA
BspLU11I 630 ACATGT A^CATGT
BsrBI 1678 CCGCTC CCGCTC(-3/-3)
BstAPI 193 GCATCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1778 CCAGAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 325 GACTTCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 173 GATATC GAT^ATC
HgaI 181 GCGTC GACGC(5/10)
Hin1I 88 GGCGCC G[GA]^CG[CT]C
HindIII 2254 AAGCTT A^AGCTT
KasI 88 GGCGCC G^GCGCC
MaeI 1974 CTAG C^TAG
MfeI 2101 CAATTG C^AATTG
MluI 179 ACGCGT A^CGCGT
MluNI 2376 TGGCCA TGG^CCA
MroI 1754 TCCGGA T^CCGGA
MscI 2376 TGGCCA TGG^CCA
MslI 990 CATGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2101 CAATTG C^AATTG
NarI 88 GGCGCC GG^CGCC
NcoI 209 CCATGG C^CATGG
NdeI 1454 CATATG CA^TATG
NspI 630 ACATGT [GA]CATG^[CT]
PflFI 2555 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2167 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1524 CACGTG CAC^GTG
PmlI 1524 CACGTG CAC^GTG
PstI 265 CTGCAG CTGCA^G
PvuI 35 CGATCG CGAT^CG
SfoI 88 GGCGCC GGC^GCC
SmaI 242 CCCGGG CCC^GGG
TaqI 2364 TCGA T^CGA
Tth111I 2555 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2364 TCGA T^CGA
Van91I 2167 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 242 CCCGGG C^CCGGG
XmaI 242 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 804 GTAC GT^AC
2060 GTAC
Aor51HI 12 AGCGCT AGC^GCT
313 AGCGCT
Asp700I 1150 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1917 GAAGAGATTC
AvaII 1065 GGACC G^G[AT]CC
1595 GGTCC
BbsI 1140 GAAGAC GAAGAC(2/6)
1860 GAAGAC
BglI 2378 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2491 GCCCAGGAGGC
BpuAI 1140 GAAGAC GAAGAC(2/6)
1860 GAAGAC
BsaBI 1108 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1255 GATAAAGATC
BseRI 42 CTCCTC GAGGAG(10/8)
1791 GAGGAG
BsmI 1051 GAATGC GAATGC(1/-1)
797 GCATTC
BssSI 1547 CACGAG CACGAG(-5/-1)
750 CTCGTG
BstYI 1433 GGATCC [GA]^GATC[CT]
1619 AGATCC
EaeI 362 CGGCCA [CT]^GGCC[GA]
2376 TGGCCA
Eco47III 12 AGCGCT AGC^GCT
313 AGCGCT
EcoRI 1462 GAATTC G^AATTC
1802 GAATTC
HincII 49 GTCAAC GT[CT]^[GA]AC
1441 GTTGAC
HindII 49 GTCAAC GT[CT]^[GA]AC
1441 GTTGAC
MamI 1108 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1255 GATAAAGATC
MflI 1433 GGATCC [GA]^GATC[CT]
1619 AGATCC
MspA1I 437 CAGCTG C[AC]G^C[GT]G
1145 CAGCTG
PvuII 437 CAGCTG CAG^CTG
1145 CAGCTG
RsaI 804 GTAC GT^AC
2060 GTAC
SexAI 347 ACCTGGT A^CC[AT]GGT
782 ACCAGGT
SmlI 2064 CTTGAG C^T[CT][GA]AG
2486 CTTGAG
XcmI 783 CCAGGTAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1446 CCAAAACTCATATGG
XhoII 1433 GGATCC [GA]^GATC[CT]
1619 AGATCC
XmnI 1150 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1917 GAAGAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcsI AflIII AluI
AlwI ApoI BanI BbvI BcnI
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI