##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccIII AclI AflII AscI
AseI AsnI Asp700I Asp718I AvrII
BbvCI BfrI BlnI BseAI BsiWI
BsmBI BspDI BspEI Bst1107I BstBI
BstZ17I ClaI CpoI DraI EcoRI
EcoT22I HindIII HpaI KpnI KspI
MfeI MluI MroI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SexAI
SfiI SfuI SnaBI SpeI SspI
SwaI TfiI Tru9I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 3558 GTCTAC GT^[AC][GT]AC
AcsI 137 GAATTT [GA]^AATT[CT]
AgeI 1261 ACCGGT A^CCGGT
AhdI 4518 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 137 GAATTT [GA]^AATT[CT]
AspEI 4518 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 949 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1610 GGATCC G^GATCC
BclI 2671 TGATCA T^GATCA
BglII 4813 AGATCT A^GATCT
BsaBI 4634 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1398 GAATGC GAATGC(1/-1)
Bsp1407I 2848 TGTACA T^GTACA
BsrDI 4888 GCAATG GCAATG(2/0)
BsrGI 2848 TGTACA T^GTACA
BssHII 997 GCGCGC G^CGCGC
BstEII 257 GGTCACC G^GT[ACGT]ACC
BstPI 257 GGTCACC G^GT[ACGT]ACC
Eam1105I 4518 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 257 GGTCACC G^GT[ACGT]ACC
EcoRV 288 GATATC GAT^ATC
FbaI 2671 TGATCA T^GATCA
FseI 1214 GGCCGGCC GGCCGG^CC
MamI 4634 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 5206 GCGGCCGC GC^GGCCGC
PaeR7I 2097 CTCGAG C^TCGAG
PflFI 949 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1261 ACCGGT A^CCGGT
PshAI 1943 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1182 CCTGCAGG CCTGCA^GG
ScaI 64 AGTACT AGT^ACT
SgrAI 611 CACCGGCG C[GA]^CCGG[CT]G
SmlI 2097 CTCGAG C^T[CT][GA]AG
SphI 1302 GCATGC GCATG^C
Sse8387I 1182 CCTGCAGG CCTGCA^GG
SspBI 2848 TGTACA T^GTACA
Tth111I 949 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2097 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AviII 3621 TGCGCA TGC^GCA
4834 TGCGCA
BcgI 747 CGATGCTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3734 CGAAGTAGGTGC
BciVI 2879 GGATAC GTATCC(6/5)
3831 GGATAC
BfaI 2452 CTAG C^TAG
2742 CTAG
BlpI 2648 GCTCAGC GC^T[ACGT]AGC
4376 GCTGAGC
Bpu1102I 2648 GCTCAGC GC^T[ACGT]AGC
4376 GCTGAGC
BsaWI 1261 ACCGGT [AT]^CCGG[AT]
3828 ACCGGA
BspHI 4759 TCATGA T^CATGA
5107 TCATGA
BspLU11I 1119 ACATGT A^CATGT
1536 ACATGT
CelII 2648 GCTCAGC GC^T[ACGT]AGC
4376 GCTGAGC
DraIII 1759 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2855 CACCCTGTG
DrdI 249 GACCCCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
830 GACGCCCTCGTC
FspI 3621 TGCGCA TGC^GCA
4834 TGCGCA
HincII 618 GTCAAC GT[CT]^[GA]AC
2214 GTCAAC
HindII 618 GTCAAC GT[CT]^[GA]AC
2214 GTCAAC
MaeI 2452 CTAG C^TAG
2742 CTAG
RcaI 4759 TCATGA T^CATGA
5107 TCATGA
SmaI 2875 CCCGGG CCC^GGG
3016 CCCGGG
StuI 1828 AGGCCT AGG^CCT
2497 AGGCCT
Tsp509I 138 AATT ^AATT
888 AATT
XmaCI 2875 CCCGGG C^CCGGG
3016 CCCGGG
XmaI 2875 CCCGGG C^CCGGG
3016 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbsI BbvI BcnI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII