##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AflIII AgeI Aor51HI AscI AseI
AsnI AviII AvrII BciVI BglII
BlnI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI Eco47III
FseI FspI HpaI MluI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 764 GACGTC GACGT^C
Acc65I 5259 GGTACC G^GTACC
AccI 4019 GTCTAC GT^[AC][GT]AC
AclI 380 AACGTT AA^CGTT
AflII 4814 CTTAAG C^TTAAG
Alw44I 4809 GTGCAC G^TGCAC
ApaLI 4809 GTGCAC G^TGCAC
Asp700I 1446 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5259 GGTACC G^GTACC
AspI 2356 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 991 TGGCCA TGG^CCA
BcgI 4177 CGAGCCATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2664 TGATCA T^GATCA
BfrI 4814 CTTAAG C^TTAAG
BlpI 5218 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5218 GCTCAGC GC^T[ACGT]AGC
BsaBI 989 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 4064 CATTGC GCAATG(2/0)
BstAPI 1642 GCACTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 554 GGTAACC G^GT[ACGT]ACC
BstPI 554 GGTAACC G^GT[ACGT]ACC
CelII 5218 GCTCAGC GC^T[ACGT]AGC
DraIII 4782 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3452 CGGCCG C^GGCCG
EclXI 3452 CGGCCG C^GGCCG
Eco52I 3452 CGGCCG C^GGCCG
EcoO65I 554 GGTAACC G^GT[ACGT]ACC
EcoRI 1498 GAATTC G^AATTC
EcoRV 2903 GATATC GAT^ATC
EcoT22I 5670 ATGCAT ATGCA^T
FbaI 2664 TGATCA T^GATCA
HincII 2014 GTTGAC GT[CT]^[GA]AC
HindII 2014 GTTGAC GT[CT]^[GA]AC
KpnI 5259 GGTACC GGTAC^C
MamI 989 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2684 CAATTG C^AATTG
MluNI 991 TGGCCA TGG^CCA
MscI 991 TGGCCA TGG^CCA
MunI 2684 CAATTG C^AATTG
NcoI 5042 CCATGG C^CATGG
NsiI 5670 ATGCAT ATGCA^T
PflFI 2356 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1375 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 380 AACGTT AA^CGTT
PvuI 2599 CGATCG CGAT^CG
StuI 4547 AGGCCT AGG^CCT
Tth111I 2356 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1446 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 1282 AAATTC [GA]^AATT[CT]
1498 GAATTC
AhdI 4576 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4601 GACGAGATGTC
ApoI 1282 AAATTC [GA]^AATT[CT]
1498 GAATTC
AspEI 4576 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4601 GACGAGATGTC
BbeI 85 GGCGCC GGCGC^C
1394 GGCGCC
BbrPI 1039 CACGTG CAC^GTG
3268 CACGTG
BsaAI 1039 CACGTG [CT]AC^GT[GA]
3268 CACGTG
BspHI 5032 TCATGA T^CATGA
5186 TCATGA
BsrFI 69 GCCGGC [GA]^CCGG[CT]
1945 GCCGGC
Cfr10I 69 GCCGGC [GA]^CCGG[CT]
1945 GCCGGC
Eam1105I 4576 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4601 GACGAGATGTC
HindIII 197 AAGCTT A^AGCTT
921 AAGCTT
KasI 85 GGCGCC G^GCGCC
1394 GGCGCC
KspI 4 CCGCGG CCGC^GG
3455 CCGCGG
NaeI 69 GCCGGC GCC^GGC
1945 GCCGGC
NarI 85 GGCGCC GG^CGCC
1394 GGCGCC
NgoMIV 69 GCCGGC G^CCGGC
1945 GCCGGC
NspI 2316 GCATGT [GA]CATG^[CT]
5311 ACATGC
PmaCI 1039 CACGTG CAC^GTG
3268 CACGTG
PmlI 1039 CACGTG CAC^GTG
3268 CACGTG
RcaI 5032 TCATGA T^CATGA
5186 TCATGA
SacII 4 CCGCGG CCGC^GG
3455 CCGCGG
SfoI 85 GGCGCC GGC^GCC
1394 GGCGCC
TaqI 2053 TCGA T^CGA
2277 TCGA
TthHB8I 2053 TCGA T^CGA
2277 TCGA
XbaI 1621 TCTAGA T^CTAGA
5284 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MroI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII XmaCI XmaI