##################################
# NO CUT ENZYMES ( 40 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I BfrI BsaBI
BsiWI BspDI BspHI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI FseI
KpnI MamI MfeI MluI MunI
NdeI NotI NspV PacI PinAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SexAI SfuI SgrAI SwaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 10129 TCCGGA T^CCGGA
AviII 10590 TGCGCA TGC^GCA
BbeI 11122 GGCGCC GGCGC^C
BcgI 533 CGATGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2523 GGATAC GTATCC(6/5)
BglII 288 AGATCT A^GATCT
BseAI 10129 TCCGGA T^CCGGA
Bsp1407I 3184 TGTACA T^GTACA
BspEI 10129 TCCGGA T^CCGGA
BspLU11I 10097 ACATGT A^CATGT
BsrGI 3184 TGTACA T^GTACA
EcoRV 3750 GATATC GAT^ATC
EcoT22I 7470 ATGCAT ATGCA^T
FspI 10590 TGCGCA TGC^GCA
HindIII 1230 AAGCTT A^AGCTT
HpaI 6402 GTTAAC GTT^AAC
KasI 11122 GGCGCC G^GCGCC
KspI 3020 CCGCGG CCGC^GG
MroI 10129 TCCGGA T^CCGGA
NarI 11122 GGCGCC GG^CGCC
NheI 8111 GCTAGC G^CTAGC
NruI 11515 TCGCGA TCG^CGA
NsiI 7470 ATGCAT ATGCA^T
PmeI 6413 GTTTAAAC GTTT^AAAC
SacII 3020 CCGCGG CCGC^GG
ScaI 8430 AGTACT AGT^ACT
SfoI 11122 GGCGCC GGC^GCC
SnaBI 6659 TACGTA TAC^GTA
SpeI 2292 ACTAGT A^CTAGT
SspBI 3184 TGTACA T^GTACA
SspI 5356 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 624 GACGTC GACGT^C
3174 GACGTC
AccI 3177 GTCTAC GT^[AC][GT]AC
8645 GTCTAC
AhdI 212 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2085 GACCCACTGTC
Aor51HI 2027 AGCGCT AGC^GCT
2662 AGCGCT
AspEI 212 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2085 GACCCACTGTC
BamHI 3352 GGATCC G^GATCC
9515 GGATCC
BclI 4753 TGATCA T^GATCA
8787 TGATCA
BsaWI 3454 ACCGGA [AT]^CCGG[AT]
10129 TCCGGA
BsmI 8472 GAATGC GAATGC(1/-1)
9486 GCATTC
BsrBI 1607 CCGCTC CCGCTC(-3/-3)
11511 CCGCTC
BsrFI 4941 GCCGGC [GA]^CCGG[CT]
8612 GCCGGC
BssSI 5062 CACGAG CACGAG(-5/-1)
7649 CTCGTG
BstAPI 10582 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
10719 GCAGTGCCTGC
Cfr10I 4941 GCCGGC [GA]^CCGG[CT]
8612 GCCGGC
DrdI 1604 GACCCGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3525 GACAGCATCGTC
EagI 3205 CGGCCG C^GGCCG
7481 CGGCCG
Eam1105I 212 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2085 GACCCACTGTC
EclXI 3205 CGGCCG C^GGCCG
7481 CGGCCG
Eco47III 2027 AGCGCT AGC^GCT
2662 AGCGCT
Eco52I 3205 CGGCCG C^GGCCG
7481 CGGCCG
EcoRI 1142 GAATTC G^AATTC
4965 GAATTC
FbaI 4753 TGATCA T^GATCA
8787 TGATCA
HincII 1014 GTTGAC GT[CT]^[GA]AC
6402 GTTAAC
HindII 1014 GTTGAC GT[CT]^[GA]AC
6402 GTTAAC
NaeI 4941 GCCGGC GCC^GGC
8612 GCCGGC
NgoMIV 4941 GCCGGC G^CCGGC
8612 GCCGGC
PaeR7I 9481 CTCGAG C^TCGAG
10537 CTCGAG
PshAI 624 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3335 GACCGATGTC
SbfI 7532 CCTGCAGG CCTGCA^GG
9865 CCTGCAGG
SfiI 2123 GGCCACCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
7272 GGCCAGCAGGGCC
SphI 2363 GCATGC GCATG^C
6862 GCATGC
Sse8387I 7532 CCTGCAGG CCTGCA^GG
9865 CCTGCAGG
XhoI 9481 CTCGAG C^TCGAG
10537 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 154 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI Asp700I
AspHI AspI AvaI AvaII AvrII
BalI BanI BanII BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoII XmaCI XmaI XmnI