##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI AscI AspHI AspI AvaI
AviII BamHI BbeI BbrPI BbvCI
BciVI BclI BcnI BfrI BglI
BglII BsaHI BseAI BsiEI BsiHKAI
BsiWI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstXI Cfr10I ClaI CpoI DraII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRV FbaI
FseI FspI Hin1I HindIII KasI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 4094 GGTACC G^GTACC
AhdI 253 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3240 CCTAAGG CC^T[ACGT]AGG
Asp718I 4094 GGTACC G^GTACC
AspEI 253 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4162 CCTAGG C^CTAGG
BalI 2324 TGGCCA TGG^CCA
BanI 4094 GGTACC G^G[CT][GA]CC
BanII 2303 GGGCTC G[GA]GC[CT]^C
BlnI 4162 CCTAGG C^CTAGG
BlpI 2018 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2018 GCTTAGC GC^T[ACGT]AGC
BsaAI 3387 CACGTA [CT]AC^GT[GA]
BsaBI 1435 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 4162 CCTAGG C^C[ACGT][ACGT]GG
BsaWI 1226 ACCGGA [AT]^CCGG[AT]
BsmBI 1571 GAGACG CGTCTC(1/5)
BspHI 233 TCATGA T^CATGA
BspMI 770 ACCTGC ACCTGC(4/8)
BsrDI 1950 GCAATG GCAATG(2/0)
Bsu36I 3240 CCTAAGG CC^T[ACGT]AGG
CelII 2018 GCTTAGC GC^T[ACGT]AGC
CfoI 13 GCGC GCG^C
DraIII 3820 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2324 TGGCCA [CT]^GGCC[GA]
Eam1105I 253 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3240 CCTAAGG CC^T[ACGT]AGG
EcoT14I 4162 CCTAGG C^C[AT][AT]GG
EcoT22I 2155 ATGCAT ATGCA^T
FauI 3170 GCGGG CCCGC(4/6)
HaeII 12 GGCGCT [GA]GCGC^[CT]
HapII 1227 CCGG C^CGG
HgaI 3204 GCGTC GACGC(5/10)
HhaI 13 GCGC GCG^C
HinP1I 13 GCGC G^CGC
HpaI 4170 GTTAAC GTT^AAC
HpaII 1227 CCGG C^CGG
KpnI 4094 GGTACC GGTAC^C
MamI 1435 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2324 TGGCCA TGG^CCA
MscI 2324 TGGCCA TGG^CCA
MslI 2966 CACTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 7 CCGCTG C[AC]G^C[GT]G
MspI 1227 CCGG C^CGG
NdeI 3510 CATATG CA^TATG
NsiI 2155 ATGCAT ATGCA^T
PacI 530 TTAATTAA TTAAT^TAA
PflMI 2409 CCAATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3695 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 233 TCATGA T^CATGA
SmlI 2096 CTCAAG C^T[CT][GA]AG
SpeI 716 ACTAGT A^CTAGT
StyI 4162 CCTAGG C^C[AT][AT]GG
Van91I 2409 CCAATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Asp700I 2588 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2836 GAAAGAATTC
AvaII 988 GGTCC G^G[AT]CC
1635 GGTCC
BcgI 1580 CGAAACCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1950 GCAATGTAATCG
BmyI 2303 GGGCTC G[AGT]GC[ACT]^C
4325 GTGCCC
BsaI 1897 GAGACC GGTCTC(1/5)
3701 GGTCTC
BseRI 495 CTCCTC GAGGAG(10/8)
775 CTCCTC
BsgI 4265 CTGCAC GTGCAG(16/14)
2074 GTGCAG
BsmFI 2188 GGGAC GGGAC(10/14)
1636 GTCCC
Bsp1286I 2303 GGGCTC G[AGT]GC[ACT]^C
4325 GTGCCC
Bst1107I 1997 GTATAC GTA^TAC
2745 GTATAC
BstYI 1537 GGATCT [GA]^GATC[CT]
3297 AGATCC
BstZ17I 1997 GTATAC GTA^TAC
2745 GTATAC
MflI 1537 GGATCT [GA]^GATC[CT]
3297 AGATCC
MwoI 1713 GCCATCAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
3547 GCAGTAATAGC
StuI 514 AGGCCT AGG^CCT
3904 AGGCCT
XhoII 1537 GGATCT [GA]^GATC[CT]
3297 AGATCC
XmnI 2588 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2836 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI BbsI BbvI BfaI BpmI
BpuAI BsiYI BslI BsmAI BsmI
BspLU11I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoNI EcoRI EcoRII Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII NlaIV NspI
PleI PshBI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I