##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccI AccIII AflII AgeI AhdI
Alw44I Aor51HI ApaLI Asp700I AspEI
AspI AviII BalI BbrPI BcgI
BciVI BfrI BlpI Bpu1102I BseAI
BsgI BsiWI BspDI BspEI BspMI
BssSI Bst1107I BstEII BstPI BstZ17I
CelII ClaI DraIII DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoT22I FspI
HpaI MluI MluNI MroI MscI
NheI NotI NruI NsiI PacI
PflFI PinAI PmaCI PmeI PmlI
PvuII SalI SapI SbfI SexAI
SfiI SgrAI SphI Sse8387I SwaI
Tth111I XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 708 GACGTC GACGT^C
Acc65I 2695 GGTACC G^GTACC
AclI 3038 AACGTT AA^CGTT
AflIII 3444 ACATGT A^C[GA][CT]GT
AocI 2771 CCTTAGG CC^T[ACGT]AGG
ApaI 772 GGGCCC GGGCC^C
AscI 421 GGCGCGCC GG^CGCGCC
AseI 2585 ATTAAT AT^TAAT
AsnI 2585 ATTAAT AT^TAAT
Asp718I 2695 GGTACC G^GTACC
AvrII 2129 CCTAGG C^CTAGG
BamHI 278 GGATCC G^GATCC
BbvCI 952 CCTCAGC CCTCAGC(-5/-2)
BclI 1746 TGATCA T^GATCA
BlnI 2129 CCTAGG C^CTAGG
BsaAI 2742 TACGTA [CT]AC^GT[GA]
BsaBI 3006 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 499 TCCGGT [AT]^CCGG[AT]
BsmBI 960 CGTCTC CGTCTC(1/5)
Bsp1407I 1422 TGTACA T^GTACA
BspHI 1312 TCATGA T^CATGA
BspLU11I 3444 ACATGT A^CATGT
BsrBI 364 GAGCGG CCGCTC(-3/-3)
BsrDI 1737 CATTGC GCAATG(2/0)
BsrGI 1422 TGTACA T^GTACA
BstBI 1530 TTCGAA TT^CGAA
BstXI 200 CCACGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2771 CCTTAGG CC^T[ACGT]AGG
CpoI 176 CGGTCCG CG^G[AT]CCG
Eco81I 2771 CCTTAGG CC^T[ACGT]AGG
EcoRV 1355 GATATC GAT^ATC
FbaI 1746 TGATCA T^GATCA
FseI 70 GGCCGGCC GGCCGG^CC
KpnI 2695 GGTACC GGTAC^C
MamI 3006 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1707 CAATTG C^AATTG
MunI 1707 CAATTG C^AATTG
NdeI 3330 CATATG CA^TATG
NspV 1530 TTCGAA TT^CGAA
PaeR7I 1477 CTCGAG C^TCGAG
PflMI 2699 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2077 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 2072 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2585 ATTAAT AT^TAAT
Psp1406I 3038 AACGTT AA^CGTT
PvuI 1497 CGATCG CGAT^CG
RcaI 1312 TCATGA T^CATGA
RsrII 176 CGGTCCG CG^G[AT]CCG
ScaI 2523 AGTACT AGT^ACT
SfuI 1530 TTCGAA TT^CGAA
SmaI 1002 CCCGGG CCC^GGG
SnaBI 2742 TACGTA TAC^GTA
SspBI 1422 TGTACA T^GTACA
SspI 3487 AATATT AAT^ATT
StuI 494 AGGCCT AGG^CCT
Van91I 2699 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1477 CTCGAG C^TCGAG
XmaCI 1002 CCCGGG C^CCGGG
XmaI 1002 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BglII 2264 AGATCT A^GATCT
2837 AGATCT
BseRI 116 GAGGAG GAGGAG(10/8)
1550 GAGGAG
BsmI 2593 GAATGC GAATGC(1/-1)
1231 GCATTC
BssHII 192 GCGCGC G^CGCGC
422 GCGCGC
DraI 2384 TTTAAA TTT^AAA
2824 TTTAAA
DraII 771 AGGGCCC [GA]G^G[ACGT]CC[CT]
2077 GGGTCCC
EagI 69 CGGCCG C^GGCCG
644 CGGCCG
EclXI 69 CGGCCG C^GGCCG
644 CGGCCG
Eco52I 69 CGGCCG C^GGCCG
644 CGGCCG
EcoNI 109 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
909 CCTTGAAAAGG
EcoO109I 771 AGGGCCC [GA]G^G[ACGT]CC[CT]
2077 GGGTCCC
HindIII 2246 AAGCTT A^AGCTT
2620 AAGCTT
HphI 23 GGTGA GGTGA(8/7)
1263 TCACC
MslI 1342 CATACACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3012 CATCAAAATG
NaeI 71 GCCGGC GCC^GGC
996 GCCGGC
NcoI 1266 CCATGG C^CATGG
2976 CCATGG
NgoMIV 71 GCCGGC G^CCGGC
996 GCCGGC
PstI 85 CTGCAG CTGCA^G
2519 CTGCAG
SacI 1465 GAGCTC GAGCT^C
2854 GAGCTC
SfcI 85 CTGCAG C^T[GA][CT]AG
2519 CTGCAG
SmlI 1477 CTCGAG C^T[CT][GA]AG
2892 CTTGAG
SpeI 3179 ACTAGT A^CTAGT
3193 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfoI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII