##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AgeI AhdI AocI Aor51HI AscI
AspEI AvaI AviII BbeI BciVI
BclI BfrI BsaBI BsaHI BsiWI
BsmBI BsmI BsoBI BspHI BspLU11I
BsrBI BssHII Bst1107I BstBI BstZ17I
Bsu36I CpoI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HpaI KasI KspI
MamI MluI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 2452 GGTACC G^GTACC
AccIII 1188 TCCGGA T^CCGGA
AflIII 2870 ACGTGT A^C[GA][CT]GT
Alw44I 777 GTGCAC G^TGCAC
ApaI 2851 GGGCCC GGGCC^C
ApaLI 777 GTGCAC G^TGCAC
AseI 2057 ATTAAT AT^TAAT
AsnI 2057 ATTAAT AT^TAAT
Asp700I 3520 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2452 GGTACC G^GTACC
AspI 802 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2869 CACGTG CAC^GTG
BcgI 1748 GCACTTGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 433 AGATCT A^GATCT
BsaAI 2869 CACGTG [CT]AC^GT[GA]
BsaWI 1188 TCCGGA [AT]^CCGG[AT]
BseAI 1188 TCCGGA T^CCGGA
BsiEI 1509 CGGTCG CG[GA][CT]^CG
Bsp1407I 2083 TGTACA T^GTACA
BspDI 590 ATCGAT AT^CGAT
BspEI 1188 TCCGGA T^CCGGA
BsrDI 3310 CATTGC GCAATG(2/0)
BsrFI 126 GCCGGC [GA]^CCGG[CT]
BsrGI 2083 TGTACA T^GTACA
BssSI 117 CTCGTG CACGAG(-5/-1)
BstAPI 2123 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3638 GGTGACC G^GT[ACGT]ACC
BstPI 3638 GGTGACC G^GT[ACGT]ACC
Cfr10I 126 GCCGGC [GA]^CCGG[CT]
ClaI 590 ATCGAT AT^CGAT
DraIII 1029 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 3338 GAAGAG CTCTTC(1/4)
EcoNI 1351 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3638 GGTGACC G^GT[ACGT]ACC
FauI 1425 CCCGC CCCGC(4/6)
HincII 1472 GTCAAC GT[CT]^[GA]AC
HindII 1472 GTCAAC GT[CT]^[GA]AC
KpnI 2452 GGTACC GGTAC^C
Ksp632I 3338 GAAGAG CTCTTC(1/4)
MfeI 2776 CAATTG C^AATTG
MroI 1188 TCCGGA T^CCGGA
MunI 2776 CAATTG C^AATTG
NaeI 126 GCCGGC GCC^GGC
NgoMIV 126 GCCGGC G^CCGGC
NspI 2096 ACATGC [GA]CATG^[CT]
PflFI 802 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2869 CACGTG CAC^GTG
PmlI 2869 CACGTG CAC^GTG
PshAI 2699 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2057 ATTAAT AT^TAAT
PstI 1837 CTGCAG CTGCA^G
SexAI 1030 ACCTGGT A^CC[AT]GGT
SmlI 1490 CTCAAG C^T[CT][GA]AG
SspBI 2083 TGTACA T^GTACA
Tth111I 802 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3520 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccII 1064 CGCG CG^CG
1310 CGCG
BamHI 294 GGATCC G^GATCC
934 GGATCC
BbsI 2889 GAAGAC GAAGAC(2/6)
2992 GTCTTC
BglI 833 GCCCTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1013 GCCGTGGTGGC
BpuAI 2889 GAAGAC GAAGAC(2/6)
2992 GTCTTC
BsaI 2697 GAGACC GGTCTC(1/5)
4 GGTCTC
BsgI 1746 CTGCAC GTGCAG(16/14)
1074 GTGCAG
BstUI 1064 CGCG CG^CG
1310 CGCG
DrdI 1235 GACAAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2508 GACTCTCATGTC
MvnI 1064 CGCG CG^CG
1310 CGCG
NcoI 409 CCATGG C^CATGG
1689 CCATGG
PflMI 1678 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1968 CCAACTACTGG
PpuMI 856 AGGTCCC [GA]G^G[AT]CC[CT]
2173 AGGTCCC
SacI 650 GAGCTC GAGCT^C
1322 GAGCTC
Van91I 1678 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1968 CCAACTACTGG
XcmI 1964 CCACCCAACTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3399 CCAAGAACAAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII AvrII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BlnI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII