##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccII AccIII AgeI AscI
AvaI AviII BalI BlpI Bpu1102I
BsaWI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BsrBI BssHII Bst1107I
BstBI BstUI BstZ17I CelII ClaI
CpoI DraIII DrdI EagI EclXI
Eco52I EcoNI FauI FseI FspI
KspI MluI MluNI MroI MscI
MspA1I MvnI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PshAI PvuI PvuII RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 5030 GGTACC G^GTACC
AccI 716 GTAGAC GT^[AC][GT]AC
AcyI 1248 GGCGCC G[GA]^CG[CT]C
Alw44I 4233 GTGCAC G^TGCAC
AocI 5745 CCTTAGG CC^T[ACGT]AGG
Aor51HI 5331 AGCGCT AGC^GCT
ApaI 5554 GGGCCC GGGCC^C
ApaLI 4233 GTGCAC G^TGCAC
Asp700I 7185 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5030 GGTACC G^GTACC
AspI 2843 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 6301 CCTAGG C^CTAGG
BamHI 1945 GGATCC G^GATCC
BbeI 1248 GGCGCC GGCGC^C
BbrPI 359 CACGTG CAC^GTG
BbvCI 1631 GCTGAGG CCTCAGC(-5/-2)
BcgI 2801 GCAACCTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 234 GTATCC GTATCC(6/5)
BclI 6767 TGATCA T^GATCA
BcnI 2347 CCGGG CC^[GC]GG
BglI 2196 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 6301 CCTAGG C^CTAGG
BsaAI 359 CACGTG [CT]AC^GT[GA]
BsaHI 1248 GGCGCC G[GA]^CG[CT]C
BsgI 1896 GTGCAG GTGCAG(16/14)
BspHI 4527 TCATGA T^CATGA
BsrFI 587 ACCGGC [GA]^CCGG[CT]
BssSI 6060 CTCGTG CACGAG(-5/-1)
BstEII 3995 GGTGACC G^GT[ACGT]ACC
BstPI 3995 GGTGACC G^GT[ACGT]ACC
Bsu36I 5745 CCTTAGG CC^T[ACGT]AGG
Cfr10I 587 ACCGGC [GA]^CCGG[CT]
Eco47III 5331 AGCGCT AGC^GCT
Eco81I 5745 CCTTAGG CC^T[ACGT]AGG
EcoO65I 3995 GGTGACC G^GT[ACGT]ACC
EcoRV 7710 GATATC GAT^ATC
FbaI 6767 TGATCA T^GATCA
Hin1I 1248 GGCGCC G[GA]^CG[CT]C
KasI 1248 GGCGCC G^GCGCC
KpnI 5030 GGTACC GGTAC^C
NarI 1248 GGCGCC GG^CGCC
NciI 2347 CCGGG CC^[GC]GG
PflFI 2843 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 6660 CCAATATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 359 CACGTG CAC^GTG
PmeI 7752 GTTTAAAC GTTT^AAAC
PmlI 359 CACGTG CAC^GTG
PpuMI 6449 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 4527 TCATGA T^CATGA
SapI 1157 GAAGAGC GCTCTTC(1/4)
ScaI 6789 AGTACT AGT^ACT
SfoI 1248 GGCGCC GGC^GCC
SpeI 2873 ACTAGT A^CTAGT
SphI 1816 GCATGC GCATG^C
SwaI 7584 ATTTAAAT ATTT^AAAT
Tth111I 2843 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 6660 CCAATATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 7185 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AciI 496 CCGC CCGC(-3/-1)
555 GCGG
AclI 1612 AACGTT AA^CGTT
3889 AACGTT
AflII 1163 CTTAAG C^TTAAG
7257 CTTAAG
AhdI 737 GACGAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1554 GACGTAGGGTC
AlwNI 877 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1526 CAGCGTCTG
AspEI 737 GACGAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1554 GACGTAGGGTC
BfrI 1163 CTTAAG C^TTAAG
7257 CTTAAG
BglII 2574 AGATCT A^GATCT
5905 AGATCT
BsaBI 2033 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6768 GATCAGCATC
BsmBI 7252 CGTCTC CGTCTC(1/5)
1552 GAGACG
BsmI 676 GAATGC GAATGC(1/-1)
6146 GCATTC
Bsp1407I 2513 TGTACA T^GTACA
7579 TGTACA
BspLU11I 7319 ACATGT A^CATGT
7412 ACATGT
BsrGI 2513 TGTACA T^GTACA
7579 TGTACA
BstAPI 1534 GCAGTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3791 GCAGTCAATGC
BstXI 2485 CCAAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5035 CCACATTGCTGG
CfoI 1249 GCGC GCG^C
5332 GCGC
EaeI 457 TGGCCG [CT]^GGCC[GA]
589 CGGCCA
Eam1105I 737 GACGAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1554 GACGTAGGGTC
EcoT22I 809 ATGCAT ATGCA^T
6234 ATGCAT
HaeII 1248 GGCGCC [GA]GCGC^[CT]
5331 AGCGCT
HapII 588 CCGG C^CGG
2347 CCGG
HgaI 1528 GCGTC GACGC(5/10)
2322 GCGTC
HhaI 1249 GCGC GCG^C
5332 GCGC
HinP1I 1249 GCGC G^CGC
5332 GCGC
HindIII 3714 AAGCTT A^AGCTT
7723 AAGCTT
HpaII 588 CCGG C^CGG
2347 CCGG
MamI 2033 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6768 GATCAGCATC
MspI 588 CCGG C^CGG
2347 CCGG
NcoI 1571 CCATGG C^CATGG
2328 CCATGG
NsiI 809 ATGCAT ATGCA^T
6234 ATGCAT
Psp1406I 1612 AACGTT AA^CGTT
3889 AACGTT
SacI 4327 GAGCTC GAGCT^C
7104 GAGCTC
SexAI 2804 ACCTGGT A^CC[AT]GGT
3999 ACCTGGT
SspBI 2513 TGTACA T^GTACA
7579 TGTACA
XbaI 3672 TCTAGA T^CTAGA
5112 TCTAGA
XcmI 1571 CCATGGGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2415 CCAATTGCTTTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI AseI AsnI AspHI AvaII
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HincII
HindII HinfI HpaI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MunI MvaI MwoI NdeI
NdeII NlaIII NlaIV NspI PleI
PshBI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII