##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AflII AgeI Alw44I
AocI Aor51HI ApaLI AscI AviII
BalI BamHI BbrPI BciVI BfrI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrFI
BssHII BstEII BstPI Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FseI FspI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NgoMIV NotI NruI NsiI
PacI PinAI PmaCI PmeI PmlI
PvuI RsrII SacI SacII SalI
SfiI SgrAI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 4448 GGTACC G^GTACC
AccII 4662 CGCG CG^CG
AclI 1206 AACGTT AA^CGTT
AhdI 4295 GACATTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2029 GGGCCC GGGCC^C
AseI 1941 ATTAAT AT^TAAT
AsnI 1941 ATTAAT AT^TAAT
Asp718I 4448 GGTACC G^GTACC
AspEI 4295 GACATTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1488 GAGCAC G[AT]GC[AT]^C
BbeI 5216 GGCGCC GGCGC^C
BcgI 3397 CGATTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3955 TGATCA T^GATCA
BglII 5058 AGATCT A^GATCT
BsiHKAI 1488 GAGCAC G[AT]GC[AT]^C
BspHI 3872 TCATGA T^CATGA
BsrBI 4495 GAGCGG CCGCTC(-3/-3)
BssSI 1696 CACGAG CACGAG(-5/-1)
Bst1107I 818 GTATAC GTA^TAC
BstAPI 3372 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 319 TTCGAA TT^CGAA
BstUI 4662 CGCG CG^CG
BstZ17I 818 GTATAC GTA^TAC
DsaI 707 CCATGG C^C[GA][CT]GG
Eam1105I 4295 GACATTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 3955 TGATCA T^GATCA
HaeII 5216 GGCGCC [GA]GCGC^[CT]
HincII 2408 GTTAAC GT[CT]^[GA]AC
HindII 2408 GTTAAC GT[CT]^[GA]AC
HindIII 4981 AAGCTT A^AGCTT
HpaI 2408 GTTAAC GTT^AAC
KasI 5216 GGCGCC G^GCGCC
KpnI 4448 GGTACC GGTAC^C
MvnI 4662 CGCG CG^CG
NarI 5216 GGCGCC GG^CGCC
NcoI 707 CCATGG C^CATGG
NheI 71 GCTAGC G^CTAGC
NspV 319 TTCGAA TT^CGAA
PaeR7I 2760 CTCGAG C^TCGAG
PshAI 4601 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1941 ATTAAT AT^TAAT
Psp1406I 1206 AACGTT AA^CGTT
RcaI 3872 TCATGA T^CATGA
SbfI 496 CCTGCAGG CCTGCA^GG
ScaI 2361 AGTACT AGT^ACT
SexAI 2777 ACCAGGT A^CC[AT]GGT
SfoI 5216 GGCGCC GGC^GCC
SfuI 319 TTCGAA TT^CGAA
SmaI 1838 CCCGGG CCC^GGG
Sse8387I 496 CCTGCAGG CCTGCA^GG
XcmI 612 CCAATTTGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2760 CTCGAG C^TCGAG
XmaCI 1838 CCCGGG C^CCGGG
XmaI 1838 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 818 GTATAC GT^[AC][GT]AC
4136 GTAGAC
AcyI 1263 GACGCC G[GA]^CG[CT]C
5216 GGCGCC
Asp700I 1611 GAATAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2662 GAACTGTTTC
AspI 95 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1905 GACTCCGTC
AvrII 2947 CCTAGG C^CTAGG
3501 CCTAGG
BlnI 2947 CCTAGG C^CTAGG
3501 CCTAGG
BsaAI 1408 CACGTA [CT]AC^GT[GA]
4147 TACGTG
BsaHI 1263 GACGCC G[GA]^CG[CT]C
5216 GGCGCC
BseRI 984 GAGGAG GAGGAG(10/8)
1606 GAGGAG
BsmBI 1910 CGTCTC CGTCTC(1/5)
1683 GAGACG
BsmI 1049 GCATTC GAATGC(1/-1)
3942 GCATTC
Bsp1407I 4077 TGTACA T^GTACA
4101 TGTACA
BspMI 4451 ACCTGC ACCTGC(4/8)
3000 GCAGGT
BsrDI 4411 CATTGC GCAATG(2/0)
3348 GCAATG
BsrGI 4077 TGTACA T^GTACA
4101 TGTACA
BstYI 1419 GGATCT [GA]^GATC[CT]
5058 AGATCT
EcoRI 778 GAATTC G^AATTC
3732 GAATTC
Hin1I 1263 GACGCC G[GA]^CG[CT]C
5216 GGCGCC
MflI 1419 GGATCT [GA]^GATC[CT]
5058 AGATCT
MslI 545 CATGATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3855 CACCAACATG
PflFI 95 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1905 GACTCCGTC
PpuMI 2715 AGGTCCT [GA]G^G[AT]CC[CT]
4122 AGGACCT
PvuII 1093 CAGCTG CAG^CTG
4168 CAGCTG
SapI 322 GAAGAGC GCTCTTC(1/4)
2770 GCTCTTC
SphI 3438 GCATGC GCATG^C
4430 GCATGC
SspBI 4077 TGTACA T^GTACA
4101 TGTACA
Tth111I 95 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1905 GACTCCGTC
XhoII 1419 GGATCT [GA]^GATC[CT]
5058 AGATCT
XmnI 1611 GAATAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2662 GAACTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI