##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AcyI AgeI AhdI
AscI AseI AsnI Asp700I AspEI
AvrII BbeI BcgI BciVI BlnI
BsaHI BsiEI BsiWI BsmBI Bsp1407I
BspHI BspLU11I BsrGI BssHII BstBI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoRI EcoT22I FseI Hin1I
HpaI KasI MfeI MluI MunI
NarI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SspBI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 998 TCCGGA T^CCGGA
AflII 7597 CTTAAG C^TTAAG
AspI 3928 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 7296 TGCGCA TGC^GCA
BamHI 6880 GGATCC G^GATCC
BclI 3307 TGATCA T^GATCA
BfrI 7597 CTTAAG C^TTAAG
BsaBI 7135 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 998 TCCGGA T^CCGGA
BspDI 3241 ATCGAT AT^CGAT
BspEI 998 TCCGGA T^CCGGA
BssSI 6312 CTCGTG CACGAG(-5/-1)
ClaI 3241 ATCGAT AT^CGAT
EcoRV 2287 GATATC GAT^ATC
FbaI 3307 TGATCA T^GATCA
FspI 7296 TGCGCA TGC^GCA
KspI 7380 CCGCGG CCGC^GG
MamI 7135 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 998 TCCGGA T^CCGGA
NaeI 5463 GCCGGC GCC^GGC
NgoMIV 5463 GCCGGC G^CCGGC
PflFI 3928 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 7380 CCGCGG CCGC^GG
SapI 6356 GCTCTTC GCTCTTC(1/4)
SmaI 7469 CCCGGG CCC^GGG
SspI 1844 AATATT AAT^ATT
Tth111I 3928 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 7469 CCCGGG C^CCGGG
XmaI 7469 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 8049 ACGTGT A^C[GA][CT]GT
8204 ACACGT
Alw44I 3593 GTGCAC G^TGCAC
7055 GTGCAC
Aor51HI 821 AGCGCT AGC^GCT
993 AGCGCT
ApaLI 3593 GTGCAC G^TGCAC
7055 GTGCAC
BglII 1133 AGATCT A^GATCT
7134 AGATCT
BsaWI 998 TCCGGA [AT]^CCGG[AT]
5706 ACCGGA
BsmI 3999 GAATGC GAATGC(1/-1)
7932 GAATGC
BsrBI 4130 CCGCTC CCGCTC(-3/-3)
4619 CCGCTC
DrdI 4678 GACCTCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6288 GACATGCCTGTC
Eco47III 821 AGCGCT AGC^GCT
993 AGCGCT
HindIII 4787 AAGCTT A^AGCTT
7600 AAGCTT
NdeI 2150 CATATG CA^TATG
2992 CATATG
NheI 1361 GCTAGC G^CTAGC
4830 GCTAGC
PaeR7I 2362 CTCGAG C^TCGAG
5037 CTCGAG
PshAI 7351 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
7981 GACTGGAGTC
SacI 350 GAGCTC GAGCT^C
5034 GAGCTC
SbfI 1956 CCTGCAGG CCTGCA^GG
3858 CCTGCAGG
SfiI 847 GGCCAGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
856 GGCCTCAGAGGCC
SphI 7299 GCATGC GCATG^C
7362 GCATGC
Sse8387I 1956 CCTGCAGG CCTGCA^GG
3858 CCTGCAGG
XhoI 2362 CTCGAG C^TCGAG
5037 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI Asp718I AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI Bst1107I BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII