##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AflII AgeI Alw44I ApaLI AscI
AseI AsnI AspI AviII BamHI
BfrI BsiWI BspDI Bst1107I BstBI
BstZ17I ClaI CpoI EcoT22I FseI
FspI HpaI KspI MfeI MluI
MunI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshBI
PvuI RsrII SacII SalI SfuI
SgrAI SnaBI SpeI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2957 GACGTC GACGT^C
AccIII 1899 TCCGGA T^CCGGA
AclI 5938 AACGTT AA^CGTT
AhdI 662 GACTGTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3606 AGCGCT AGC^GCT
Asp700I 2317 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 662 GACTGTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 229 GGCGCC GGCGC^C
BbsI 1102 GAAGAC GAAGAC(2/6)
BcgI 307 GCACAGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 220 GGATAC GTATCC(6/5)
BpuAI 1102 GAAGAC GAAGAC(2/6)
BsaBI 2637 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1899 TCCGGA [AT]^CCGG[AT]
BseAI 1899 TCCGGA T^CCGGA
BsmI 5765 GAATGC GAATGC(1/-1)
Bsp1407I 6315 TGTACA T^GTACA
BspEI 1899 TCCGGA T^CCGGA
BspHI 5855 TCATGA T^CATGA
BsrBI 2377 GAGCGG CCGCTC(-3/-3)
BsrGI 6315 TGTACA T^GTACA
BssHII 1710 GCGCGC G^CGCGC
BssSI 1438 CTCGTG CACGAG(-5/-1)
DrdI 2867 GACACCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 662 GACTGTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3606 AGCGCT AGC^GCT
EcoRV 2426 GATATC GAT^ATC
HindIII 4771 AAGCTT A^AGCTT
KasI 229 GGCGCC G^GCGCC
MamI 2637 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1899 TCCGGA T^CCGGA
NaeI 4043 GCCGGC GCC^GGC
NarI 229 GGCGCC GG^CGCC
NgoMIV 4043 GCCGGC G^CCGGC
NheI 5103 GCTAGC G^CTAGC
PaeR7I 3089 CTCGAG C^TCGAG
Psp1406I 5938 AACGTT AA^CGTT
RcaI 5855 TCATGA T^CATGA
SapI 3428 GAAGAGC GCTCTTC(1/4)
SbfI 2947 CCTGCAGG CCTGCA^GG
ScaI 5178 AGTACT AGT^ACT
SexAI 3462 ACCTGGT A^CC[AT]GGT
SfoI 229 GGCGCC GGC^GCC
SmlI 3089 CTCGAG C^T[CT][GA]AG
SphI 911 GCATGC GCATG^C
Sse8387I 2947 CCTGCAGG CCTGCA^GG
SspBI 6315 TGTACA T^GTACA
XhoI 3089 CTCGAG C^TCGAG
XmnI 2317 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 3654 GGTACC G^GTACC
5800 GGTACC
Asp718I 3654 GGTACC G^GTACC
5800 GGTACC
AvrII 2885 CCTAGG C^CTAGG
4946 CCTAGG
BclI 1034 TGATCA T^GATCA
3435 TGATCA
BlnI 2885 CCTAGG C^CTAGG
4946 CCTAGG
BlpI 770 GCTCAGC GC^T[ACGT]AGC
3359 GCTGAGC
Bpu1102I 770 GCTCAGC GC^T[ACGT]AGC
3359 GCTGAGC
BsaI 1545 GAGACC GGTCTC(1/5)
2875 GGTCTC
BsiEI 2914 CGGCCG CG[GA][CT]^CG
3533 CGGCCG
BspLU11I 3777 ACATGT A^CATGT
4564 ACATGT
BsrFI 4043 GCCGGC [GA]^CCGG[CT]
5422 ACCGGC
BstAPI 254 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3069 GCAAAGTCTGC
BstEII 122 GGTCACC G^GT[ACGT]ACC
439 GGTGACC
BstPI 122 GGTCACC G^GT[ACGT]ACC
439 GGTGACC
CelII 770 GCTCAGC GC^T[ACGT]AGC
3359 GCTGAGC
Cfr10I 4043 GCCGGC [GA]^CCGG[CT]
5422 ACCGGC
DraI 6031 TTTAAA TTT^AAA
6073 TTTAAA
EagI 2914 CGGCCG C^GGCCG
3533 CGGCCG
EclXI 2914 CGGCCG C^GGCCG
3533 CGGCCG
Eco52I 2914 CGGCCG C^GGCCG
3533 CGGCCG
EcoO65I 122 GGTCACC G^GT[ACGT]ACC
439 GGTGACC
FbaI 1034 TGATCA T^GATCA
3435 TGATCA
KpnI 3654 GGTACC GGTAC^C
5800 GGTACC
NdeI 723 CATATG CA^TATG
3488 CATATG
PshAI 116 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5040 GACAGCGGTC
SfiI 1883 GGCCTGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2906 GGCCAGGGCGGCC
SspI 4572 AATATT AAT^ATT
6016 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApaI ApoI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbvCI BbvI BcnI BfaI BglI
BglII BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII
XmaCI XmaI