##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
Alw44I ApaLI AscI Asp718I AvaI
AviII BamHI BbrPI BglII BsaAI
BsaBI BsiWI BsmBI BsoBI Bsp1407I
BsrGI BssHII Bst1107I BstBI BstZ17I
CpoI EcoRI EcoRV EcoT22I FseI
FspI HpaI KpnI MaeII MamI
MluI NdeI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmlI Psp1406I PvuI RsrII SalI
ScaI SexAI SfuI SgrAI SmaI
SnaBI SpeI SspBI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AflII 103 CTTAAG C^TTAAG
AflIII 2880 ACATGT A^C[GA][CT]GT
Aor51HI 2216 AGCGCT AGC^GCT
AseI 2756 ATTAAT AT^TAAT
AsnI 2756 ATTAAT AT^TAAT
Asp700I 4985 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2228 TGGCCA TGG^CCA
BbeI 2105 GGCGCC GGCGC^C
BcgI 2389 CGAGAGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3116 GGATAC GTATCC(6/5)
BfrI 103 CTTAAG C^TTAAG
BsiEI 1698 CGGCCG CG[GA][CT]^CG
BsmI 3795 GAATGC GAATGC(1/-1)
BspDI 3877 ATCGAT AT^CGAT
BspLU11I 2880 ACATGT A^CATGT
BsrDI 2949 CATTGC GCAATG(2/0)
ClaI 3877 ATCGAT AT^CGAT
EagI 1698 CGGCCG C^GGCCG
EclXI 1698 CGGCCG C^GGCCG
Eco47III 2216 AGCGCT AGC^GCT
Eco52I 1698 CGGCCG C^GGCCG
KasI 2105 GGCGCC G^GCGCC
MfeI 384 CAATTG C^AATTG
MluNI 2228 TGGCCA TGG^CCA
MscI 2228 TGGCCA TGG^CCA
MunI 384 CAATTG C^AATTG
NarI 2105 GGCGCC GG^CGCC
NheI 2666 GCTAGC G^CTAGC
PmeI 3311 GTTTAAAC GTTT^AAAC
PshBI 2756 ATTAAT AT^TAAT
SfoI 2105 GGCGCC GGC^GCC
SphI 4829 GCATGC GCATG^C
XbaI 3076 TCTAGA T^CTAGA
XmnI 4985 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 897 TCCGGA T^CCGGA
1436 TCCGGA
AcsI 3032 GAATTT [GA]^AATT[CT]
5311 AAATTT
AhdI 639 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2360 GACTCCCTGTC
ApoI 3032 GAATTT [GA]^AATT[CT]
5311 AAATTT
AspEI 639 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2360 GACTCCCTGTC
AvrII 3388 CCTAGG C^CTAGG
3553 CCTAGG
BclI 490 TGATCA T^GATCA
3334 TGATCA
BlnI 3388 CCTAGG C^CTAGG
3553 CCTAGG
BsaWI 897 TCCGGA [AT]^CCGG[AT]
1436 TCCGGA
BseAI 897 TCCGGA T^CCGGA
1436 TCCGGA
BspEI 897 TCCGGA T^CCGGA
1436 TCCGGA
BsrFI 2473 GCCGGC [GA]^CCGG[CT]
2650 GCCGGC
Cfr10I 2473 GCCGGC [GA]^CCGG[CT]
2650 GCCGGC
DraIII 4114 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
4708 CACAAGGTG
DrdI 242 GACCTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3691 GACACAGCTGTC
Eam1105I 639 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2360 GACTCCCTGTC
FbaI 490 TGATCA T^GATCA
3334 TGATCA
HincII 636 GTTGAC GT[CT]^[GA]AC
1452 GTCAAC
HindII 636 GTTGAC GT[CT]^[GA]AC
1452 GTCAAC
HindIII 1473 AAGCTT A^AGCTT
4987 AAGCTT
KspI 1622 CCGCGG CCGC^GG
1681 CCGCGG
MroI 897 TCCGGA T^CCGGA
1436 TCCGGA
NaeI 2473 GCCGGC GCC^GGC
2650 GCCGGC
NgoMIV 2473 GCCGGC G^CCGGC
2650 GCCGGC
PflMI 729 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1087 CCAATGACTGG
PshAI 3885 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4124 GACCACAGTC
SacI 1990 GAGCTC GAGCT^C
4631 GAGCTC
SacII 1622 CCGCGG CCGC^GG
1681 CCGCGG
SfiI 2295 GGCCGAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2304 GGCCGAGGTGGCC
SspI 3082 AATATT AAT^ATT
3674 AATATT
Van91I 729 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1087 CCAATGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AspI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrBI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PleI PpuMI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SmlI Sse8387I StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XcmI
XhoII