##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AclI AflII AscI AseI
AsnI Asp700I Asp718I AviII BciVI
BclI BfrI BsaBI BsiWI BspDI
BspHI BspLU11I Bst1107I BstBI BstZ17I
ClaI CpoI EcoRV EcoT22I FbaI
FseI FspI HpaI KpnI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 19 enzymes) #
###################################
AatII 3187 GACGTC GACGT^C
AcsI 5284 GAATTC [GA]^AATT[CT]
AgeI 912 ACCGGT A^CCGGT
Alw44I 4648 GTGCAC G^TGCAC
ApaLI 4648 GTGCAC G^TGCAC
ApoI 5284 GAATTC [GA]^AATT[CT]
BamHI 144 GGATCC G^GATCC
BglII 829 AGATCT A^GATCT
BstEII 3204 GGTCACC G^GT[ACGT]ACC
BstPI 3204 GGTCACC G^GT[ACGT]ACC
DraI 2271 TTTAAA TTT^AAA
DraIII 3748 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 3204 GGTCACC G^GT[ACGT]ACC
EcoRI 5284 GAATTC G^AATTC
KspI 3811 CCGCGG CCGC^GG
PinAI 912 ACCGGT A^CCGGT
SacI 5180 GAGCTC GAGCT^C
SacII 3811 CCGCGG CCGC^GG
TfiI 3052 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 772 GTCTAC GT^[AC][GT]AC
902 GTCTAC
AccIII 851 TCCGGA T^CCGGA
1791 TCCGGA
BbsI 476 GTCTTC GAAGAC(2/6)
5060 GTCTTC
BlpI 2898 GCTCAGC GC^T[ACGT]AGC
3087 GCTCAGC
Bpu1102I 2898 GCTCAGC GC^T[ACGT]AGC
3087 GCTCAGC
BpuAI 476 GTCTTC GAAGAC(2/6)
5060 GTCTTC
BseAI 851 TCCGGA T^CCGGA
1791 TCCGGA
BsmBI 2087 CGTCTC CGTCTC(1/5)
3549 CGTCTC
Bsp1407I 972 TGTACA T^GTACA
4980 TGTACA
BspEI 851 TCCGGA T^CCGGA
1791 TCCGGA
BsrGI 972 TGTACA T^GTACA
4980 TGTACA
BssHII 67 GCGCGC G^CGCGC
120 GCGCGC
BstYI 144 GGATCC [GA]^GATC[CT]
829 AGATCT
CelII 2898 GCTCAGC GC^T[ACGT]AGC
3087 GCTCAGC
EcoNI 754 CCTCTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5249 CCTCCCGAAGG
HincII 1447 GTTGAC GT[CT]^[GA]AC
4697 GTTGAC
HindII 1447 GTTGAC GT[CT]^[GA]AC
4697 GTTGAC
HindIII 2930 AAGCTT A^AGCTT
5101 AAGCTT
MflI 144 GGATCC [GA]^GATC[CT]
829 AGATCT
MroI 851 TCCGGA T^CCGGA
1791 TCCGGA
NaeI 1433 GCCGGC GCC^GGC
4922 GCCGGC
NgoMIV 1433 GCCGGC G^CCGGC
4922 GCCGGC
PaeR7I 91 CTCGAG C^TCGAG
3902 CTCGAG
SfiI 3130 GGCCAGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4015 GGCCCTGGAGGCC
SphI 3450 GCATGC GCATG^C
3994 GCATGC
SspBI 972 TGTACA T^GTACA
4980 TGTACA
Tsp509I 2626 AATT ^AATT
5285 AATT
XhoI 91 CTCGAG C^TCGAG
3902 CTCGAG
XhoII 144 GGATCC [GA]^GATC[CT]
829 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AhdI AluI AlwI AlwNI AocI
Aor51HI ApaI AspEI AspHI AspI
AvaI AvaII AvrII BalI BanI
BanII BbeI BbrPI BbvCI BbvI
BcgI BcnI BfaI BglI BlnI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I TspRI Tth111I
TthHB8I Van91I XcmI XmaCI XmaI