##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
Acc65I AflII Aor51HI AscI AseI
AsnI Asp700I Asp718I BamHI BclI
BfrI BglII BsaBI BsiWI BspDI
BsrDI Bst1107I BstBI BstZ17I ClaI
CpoI Eco47III EcoRV EcoT22I FbaI
FseI KpnI MamI MfeI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshBI
PvuI RsrII SfuI SgrAI SnaBI
SphI SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 1990 TCCGGA T^CCGGA
AclI 3691 AACGTT AA^CGTT
AgeI 3176 ACCGGT A^CCGGT
AhdI 2371 GACCACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2371 GACCACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3629 CCTAGG C^CTAGG
BlnI 3629 CCTAGG C^CTAGG
BseAI 1990 TCCGGA T^CCGGA
BsmBI 232 CGTCTC CGTCTC(1/5)
BsmI 1453 GCATTC GAATGC(1/-1)
Bsp1407I 909 TGTACA T^GTACA
BspEI 1990 TCCGGA T^CCGGA
BspHI 3701 TCATGA T^CATGA
BspLU11I 912 ACATGT A^CATGT
BsrBI 1105 CCGCTC CCGCTC(-3/-3)
BsrGI 909 TGTACA T^GTACA
BssSI 3896 CACGAG CACGAG(-5/-1)
BstAPI 4672 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 4111 TTTAAA TTT^AAA
DraIII 4381 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2489 GACTGGCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2371 GACCACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 5098 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4361 GAATTC G^AATTC
HindIII 4573 AAGCTT A^AGCTT
HpaI 3868 GTTAAC GTT^AAC
MluI 117 ACGCGT A^CGCGT
MroI 1990 TCCGGA T^CCGGA
PflMI 3591 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3176 ACCGGT A^CCGGT
Psp1406I 3691 AACGTT AA^CGTT
RcaI 3701 TCATGA T^CATGA
SacI 3997 GAGCTC GAGCT^C
SalI 39 GTCGAC G^TCGAC
SexAI 4342 ACCAGGT A^CC[AT]GGT
SfiI 4223 GGCCATCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 909 TGTACA T^GTACA
Van91I 3591 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2380 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 1420 GACGTC GACGT^C
3125 GACGTC
AflIII 117 ACGCGT A^C[GA][CT]GT
912 ACATGT
ApaI 3654 GGGCCC GGGCC^C
4289 GGGCCC
BbeI 607 GGCGCC GGCGC^C
2805 GGCGCC
BbrPI 1243 CACGTG CAC^GTG
2791 CACGTG
BciVI 3338 GGATAC GTATCC(6/5)
5189 GGATAC
BglI 1118 GCCGCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4224 GCCATCCCGGC
BsaAI 1243 CACGTG [CT]AC^GT[GA]
2791 CACGTG
KasI 607 GGCGCC G^GCGCC
2805 GGCGCC
KspI 1175 CCGCGG CCGC^GG
2087 CCGCGG
NaeI 173 GCCGGC GCC^GGC
604 GCCGGC
NarI 607 GGCGCC GG^CGCC
2805 GGCGCC
NgoMIV 173 GCCGGC G^CCGGC
604 GCCGGC
NspI 912 ACATGT [GA]CATG^[CT]
3921 GCATGT
PmaCI 1243 CACGTG CAC^GTG
2791 CACGTG
PmlI 1243 CACGTG CAC^GTG
2791 CACGTG
PshAI 2916 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4242 GACCCCAGTC
SacII 1175 CCGCGG CCGC^GG
2087 CCGCGG
SapI 398 GCTCTTC GCTCTTC(1/4)
2202 GCTCTTC
SbfI 1043 CCTGCAGG CCTGCA^GG
4263 CCTGCAGG
ScaI 2511 AGTACT AGT^ACT
4271 AGTACT
SfoI 607 GGCGCC GGC^GCC
2805 GGCGCC
SmlI 1381 CTCAAG C^T[CT][GA]AG
5294 CTTGAG
SpeI 2775 ACTAGT A^CTAGT
3194 ACTAGT
Sse8387I 1043 CCTGCAGG CCTGCA^GG
4263 CCTGCAGG
StuI 2158 AGGCCT AGG^CCT
3166 AGGCCT
TaqI 40 TCGA T^CGA
5308 TCGA
TfiI 2564 GATTC G^A[AT]TC
4698 GATTC
TthHB8I 40 TCGA T^CGA
5308 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AspHI AspI
AvaI AvaII AviII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssHII
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflFI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I XcmI XhoII XmaCI XmaI