##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AocI AscI
Asp718I AspEI AspI AvrII BbrPI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiWI BspDI BspEI BspLU11I BspMI
BssSI BstXI Bsu36I CelII ClaI
CpoI DrdI Eam1105I Eco81I EcoRV
FseI HindIII KpnI MamI MfeI
MluI MroI MunI NheI NotI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SalI SapI SbfI SexAI SfiI
SgrAI SpeI Sse8387I SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Alw44I 1542 GTGCAC G^TGCAC
Aor51HI 3362 AGCGCT AGC^GCT
ApaI 97 GGGCCC GGGCC^C
ApaLI 1542 GTGCAC G^TGCAC
AvaI 93 CCCGGG C^[CT]CG[GA]G
AviII 3306 TGCGCA TGC^GCA
BamHI 4001 GGATCC G^GATCC
BbvCI 3818 GCTGAGG CCTCAGC(-5/-2)
BclI 3202 TGATCA T^GATCA
BglI 3273 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1695 TACGTA [CT]AC^GT[GA]
BsgI 3695 GTGCAG GTGCAG(16/14)
BsiEI 109 CGGCCG CG[GA][CT]^CG
BsmBI 311 GAGACG CGTCTC(1/5)
BsoBI 93 CCCGGG C^[CT]CG[GA]G
Bsp1407I 4108 TGTACA T^GTACA
BspHI 1402 TCATGA T^CATGA
BsrBI 3325 CCGCTC CCGCTC(-3/-3)
BsrGI 4108 TGTACA T^GTACA
BssHII 381 GCGCGC G^CGCGC
BstAPI 1946 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1598 TTCGAA TT^CGAA
BstEII 4537 GGTTACC G^GT[ACGT]ACC
BstPI 4537 GGTTACC G^GT[ACGT]ACC
DraIII 1842 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 109 CGGCCG C^GGCCG
EclXI 109 CGGCCG C^GGCCG
Eco47III 3362 AGCGCT AGC^GCT
Eco52I 109 CGGCCG C^GGCCG
EcoNI 4349 CCTCTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4537 GGTTACC G^GT[ACGT]ACC
EcoRI 661 GAATTC G^AATTC
EcoT22I 3827 ATGCAT ATGCA^T
FbaI 3202 TGATCA T^GATCA
FspI 3306 TGCGCA TGC^GCA
HincII 5053 GTTAAC GT[CT]^[GA]AC
HindII 5053 GTTAAC GT[CT]^[GA]AC
HpaI 5053 GTTAAC GTT^AAC
KspI 2651 CCGCGG CCGC^GG
NdeI 3173 CATATG CA^TATG
NruI 432 TCGCGA TCG^CGA
NsiI 3827 ATGCAT ATGCA^T
NspV 1598 TTCGAA TT^CGAA
PacI 4432 TTAATTAA TTAAT^TAA
PflMI 4747 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1402 TCATGA T^CATGA
SacII 2651 CCGCGG CCGC^GG
ScaI 3029 AGTACT AGT^ACT
SfuI 1598 TTCGAA TT^CGAA
SmaI 93 CCCGGG CCC^GGG
SnaBI 1695 TACGTA TAC^GTA
SphI 3309 GCATGC GCATG^C
SspBI 4108 TGTACA T^GTACA
StuI 273 AGGCCT AGG^CCT
Van91I 4747 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 189 CCAGTGCGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 93 CCCGGG C^CCGGG
XmaI 93 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AseI 4266 ATTAAT AT^TAAT
4435 ATTAAT
AsnI 4266 ATTAAT AT^TAAT
4435 ATTAAT
BalI 615 TGGCCA TGG^CCA
945 TGGCCA
BanI 76 GGCGCC G^G[CT][GA]CC
2647 GGCGCC
BbeI 76 GGCGCC GGCGC^C
2647 GGCGCC
BsaI 2486 GAGACC GGTCTC(1/5)
2406 GGTCTC
BsrDI 4609 CATTGC GCAATG(2/0)
3140 GCAATG
Bst1107I 2167 GTATAC GTA^TAC
4100 GTATAC
BstZ17I 2167 GTATAC GTA^TAC
4100 GTATAC
DraI 4138 TTTAAA TTT^AAA
4272 TTTAAA
KasI 76 GGCGCC G^GCGCC
2647 GGCGCC
MluNI 615 TGGCCA TGG^CCA
945 TGGCCA
MscI 615 TGGCCA TGG^CCA
945 TGGCCA
NaeI 1 GCCGGC GCC^GGC
391 GCCGGC
NarI 76 GGCGCC GG^CGCC
2647 GGCGCC
NgoMIV 1 GCCGGC G^CCGGC
391 GCCGGC
NspI 2875 ACATGC [GA]CATG^[CT]
3309 GCATGC
PshBI 4266 ATTAAT AT^TAAT
4435 ATTAAT
SfoI 76 GGCGCC GGC^GCC
2647 GGCGCC
SspI 4220 AATATT AAT^ATT
4238 AATATT
XbaI 3680 TCTAGA T^CTAGA
4725 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI