##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI AocI AscI AseI AsnI
Asp700I AspEI AviII BamHI BbrPI
BcgI BciVI BfrI BsaBI BsiWI
BspLU11I BsrFI BssHII Bst1107I BstBI
BstZ17I Bsu36I Cfr10I CpoI DraI
Eam1105I Eco81I EcoT22I FseI FspI
HindIII HpaI KspI MamI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 899 GGTACC G^GTACC
AccI 2220 GTCTAC GT^[AC][GT]AC
AccIII 3329 TCCGGA T^CCGGA
Asp718I 899 GGTACC G^GTACC
AspI 2462 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3231 GGCGCC GGCGC^C
BclI 3198 TGATCA T^GATCA
BglII 657 AGATCT A^GATCT
BsaAI 3145 TACGTG [CT]AC^GT[GA]
BseAI 3329 TCCGGA T^CCGGA
BsiEI 2 CGGCCG CG[GA][CT]^CG
BsmBI 434 CGTCTC CGTCTC(1/5)
Bsp1407I 213 TGTACA T^GTACA
BspDI 398 ATCGAT AT^CGAT
BspEI 3329 TCCGGA T^CCGGA
BspHI 2456 TCATGA T^CATGA
BspMI 721 GCAGGT ACCTGC(4/8)
BsrDI 3752 GCAATG GCAATG(2/0)
BsrGI 213 TGTACA T^GTACA
BssSI 71 CACGAG CACGAG(-5/-1)
BstAPI 3042 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2433 GGTGACC G^GT[ACGT]ACC
BstPI 2433 GGTGACC G^GT[ACGT]ACC
ClaI 398 ATCGAT AT^CGAT
EagI 2 CGGCCG C^GGCCG
EarI 3544 GAAGAG CTCTTC(1/4)
EclXI 2 CGGCCG C^GGCCG
Eco52I 2 CGGCCG C^GGCCG
EcoO65I 2433 GGTGACC G^GT[ACGT]ACC
EcoRI 307 GAATTC G^AATTC
EcoRV 884 GATATC GAT^ATC
FbaI 3198 TGATCA T^GATCA
HincII 974 GTCAAC GT[CT]^[GA]AC
HindII 974 GTCAAC GT[CT]^[GA]AC
KasI 3231 GGCGCC G^GCGCC
KpnI 899 GGTACC GGTAC^C
Ksp632I 3544 GAAGAG CTCTTC(1/4)
MroI 3329 TCCGGA T^CCGGA
MseI 780 TTAA T^TAA
NarI 3231 GGCGCC GG^CGCC
NotI 1 GCGGCCGC GC^GGCCGC
NruI 535 TCGCGA TCG^CGA
PaeR7I 3413 CTCGAG C^TCGAG
PflFI 2462 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2307 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2456 TCATGA T^CATGA
ScaI 2784 AGTACT AGT^ACT
SexAI 207 ACCTGGT A^CC[AT]GGT
SfoI 3231 GGCGCC GGC^GCC
SmlI 3413 CTCGAG C^T[CT][GA]AG
SspBI 213 TGTACA T^GTACA
Tru9I 780 TTAA T^TAA
Tth111I 2462 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2025 TCTAGA T^CTAGA
XcmI 3063 CCAGCCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3413 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 133 CGCG CG^CG
536 CGCG
AcsI 307 GAATTC [GA]^AATT[CT]
767 GAATTT
Alw44I 254 GTGCAC G^TGCAC
2898 GTGCAC
AlwI 2209 GATCC GGATC(4/5)
2823 GATCC
Aor51HI 681 AGCGCT AGC^GCT
999 AGCGCT
ApaLI 254 GTGCAC G^TGCAC
2898 GTGCAC
ApoI 307 GAATTC [GA]^AATT[CT]
767 GAATTT
AvrII 1181 CCTAGG C^CTAGG
3435 CCTAGG
BlnI 1181 CCTAGG C^CTAGG
3435 CCTAGG
BsaWI 1027 ACCGGA [AT]^CCGG[AT]
3329 TCCGGA
BsmI 2274 GCATTC GAATGC(1/-1)
3631 GCATTC
BstUI 133 CGCG CG^CG
536 CGCG
BstXI 807 CCAACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1916 CCAGTGGTATGG
DraIII 2624 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2646 CACGGGGTG
DrdI 338 GACAAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1099 GACAGCATGGTC
Eco47III 681 AGCGCT AGC^GCT
999 AGCGCT
MaeII 3146 ACGT A^CGT
3404 ACGT
MslI 1331 CACATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2499 CATCACTGTG
MvnI 133 CGCG CG^CG
536 CGCG
SphI 1303 GCATGC GCATG^C
2511 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI