##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
BbrPI BciVI BclI BfrI BsaBI
BsaI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII BstBI
BstEII BstPI ClaI DraI Eam1105I
Eco47III EcoO65I EcoRV EcoT22I FbaI
FseI FspI HpaI MamI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SalI
SfiI SfuI SnaBI SpeI SspBI
SspI SwaI Tth111I Van91I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 1046 GGTACC G^GTACC
AccIII 219 TCCGGA T^CCGGA
Asp718I 1046 GGTACC G^GTACC
AvrII 1618 CCTAGG C^CTAGG
BbsI 1771 GTCTTC GAAGAC(2/6)
BglII 4565 AGATCT A^GATCT
BlnI 1618 CCTAGG C^CTAGG
BpuAI 1771 GTCTTC GAAGAC(2/6)
BsaAI 2583 TACGTG [CT]AC^GT[GA]
BsaWI 219 TCCGGA [AT]^CCGG[AT]
BseAI 219 TCCGGA T^CCGGA
BspEI 219 TCCGGA T^CCGGA
BsrDI 3239 GCAATG GCAATG(2/0)
BssSI 3490 CTCGTG CACGAG(-5/-1)
Bst1107I 2580 GTATAC GTA^TAC
BstZ17I 2580 GTATAC GTA^TAC
CpoI 487 CGGTCCG CG^G[AT]CCG
DrdI 1457 GACTTTTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 549 CGGCCG C^GGCCG
EclXI 549 CGGCCG C^GGCCG
Eco52I 549 CGGCCG C^GGCCG
EcoNI 4050 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 87 GACGC GACGC(5/10)
HincII 3142 GTCAAC GT[CT]^[GA]AC
HindII 3142 GTCAAC GT[CT]^[GA]AC
KpnI 1046 GGTACC GGTAC^C
MroI 219 TCCGGA T^CCGGA
MseI 4109 TTAA T^TAA
NdeI 1373 CATATG CA^TATG
PaeR7I 2832 CTCGAG C^TCGAG
RsrII 487 CGGTCCG CG^G[AT]CCG
SapI 3678 GAAGAGC GCTCTTC(1/4)
SbfI 1204 CCTGCAGG CCTGCA^GG
ScaI 4103 AGTACT AGT^ACT
SgrAI 227 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 1204 CCTGCAGG CCTGCA^GG
StuI 3091 AGGCCT AGG^CCT
Tru9I 4109 TTAA T^TAA
XhoI 2832 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 2580 GTATAC GT^[AC][GT]AC
3284 GTCTAC
AcsI 1879 GAATTC [GA]^AATT[CT]
2938 GAATTC
AflIII 2584 ACGTGT A^C[GA][CT]GT
3377 ACACGT
ApoI 1879 GAATTC [GA]^AATT[CT]
2938 GAATTC
BalI 2742 TGGCCA TGG^CCA
3898 TGGCCA
BsiEI 549 CGGCCG CG[GA][CT]^CG
3123 CGACCG
BsmBI 1957 CGTCTC CGTCTC(1/5)
2043 CGTCTC
DraIII 2361 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4364 CACACTGTG
EcoRI 1879 GAATTC G^AATTC
2938 GAATTC
MaeII 2584 ACGT A^CGT
3379 ACGT
MluNI 2742 TGGCCA TGG^CCA
3898 TGGCCA
MscI 2742 TGGCCA TGG^CCA
3898 TGGCCA
NaeI 228 GCCGGC GCC^GGC
3830 GCCGGC
NcoI 3409 CCATGG C^CATGG
3439 CCATGG
NgoMIV 228 GCCGGC G^CCGGC
3830 GCCGGC
PpuMI 1464 AGGTCCC [GA]G^G[AT]CC[CT]
3770 GGGTCCT
SexAI 275 ACCTGGT A^CC[AT]GGT
3840 ACCAGGT
SphI 2202 GCATGC GCATG^C
2730 GCATGC
TaqI 1690 TCGA T^CGA
2833 TCGA
TthHB8I 1690 TCGA T^CGA
2833 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BamHI BanI BanII
BbeI BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StyI TfiI
TseI Tsp45I Tsp509I TspRI XcmI
XhoII XmaCI XmaI