##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI ApoI AscI AseI
AsnI Asp700I AspI AviII AvrII
BbrPI BciVI BfrI BglII BlnI
BsaBI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HpaI KspI MamI
MfeI MroI MunI NcoI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 1704 GGTACC G^GTACC
AhdI 2017 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1644 GTGCAC G^TGCAC
ApaLI 1644 GTGCAC G^TGCAC
Asp718I 1704 GGTACC G^GTACC
AspEI 2017 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1376 GGATCC G^GATCC
BlpI 1817 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1817 GCTCAGC GC^T[ACGT]AGC
BsaAI 1652 TACGTG [CT]AC^GT[GA]
BsmI 3716 GCATTC GAATGC(1/-1)
CelII 1817 GCTCAGC GC^T[ACGT]AGC
Eam1105I 2017 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII 3923 AAGCTT A^AGCTT
KpnI 1704 GGTACC GGTAC^C
MslI 1312 CACAGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1601 GCCGGC GCC^GGC
NgoMIV 1601 GCCGGC G^CCGGC
ScaI 1692 AGTACT AGT^ACT
SfiI 1561 GGCCCGTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 1932 AGGCCT AGG^CCT
Tsp509I 3941 AATT ^AATT
XcmI 1428 CCAAGGTCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AocI 568 CCTAAGG CC^T[ACGT]AGG
2705 CCTAAGG
Aor51HI 83 AGCGCT AGC^GCT
2220 AGCGCT
BalI 1575 TGGCCA TGG^CCA
1638 TGGCCA
BbeI 645 GGCGCC GGCGC^C
2782 GGCGCC
BclI 1456 TGATCA T^GATCA
3558 TGATCA
BglI 1562 GCCCGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3545 GCCCTCAAGGC
BsgI 1024 GTGCAG GTGCAG(16/14)
3161 GTGCAG
BsmBI 3619 CGTCTC CGTCTC(1/5)
1526 GAGACG
BspHI 410 TCATGA T^CATGA
2547 TCATGA
BstEII 1093 GGTGACC G^GT[ACGT]ACC
3230 GGTGACC
BstPI 1093 GGTGACC G^GT[ACGT]ACC
3230 GGTGACC
Bsu36I 568 CCTAAGG CC^T[ACGT]AGG
2705 CCTAAGG
DraIII 541 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2678 CACCCTGTG
Eco47III 83 AGCGCT AGC^GCT
2220 AGCGCT
Eco81I 568 CCTAAGG CC^T[ACGT]AGG
2705 CCTAAGG
EcoO65I 1093 GGTGACC G^GT[ACGT]ACC
3230 GGTGACC
FbaI 1456 TGATCA T^GATCA
3558 TGATCA
KasI 645 GGCGCC G^GCGCC
2782 GGCGCC
MluI 977 ACGCGT A^CGCGT
3114 ACGCGT
MluNI 1575 TGGCCA TGG^CCA
1638 TGGCCA
MscI 1575 TGGCCA TGG^CCA
1638 TGGCCA
NarI 645 GGCGCC GG^CGCC
2782 GGCGCC
NdeI 595 CATATG CA^TATG
2732 CATATG
NheI 268 GCTAGC G^CTAGC
2405 GCTAGC
NspI 1779 GCATGT [GA]CATG^[CT]
3495 GCATGT
PaeR7I 766 CTCGAG C^TCGAG
2903 CTCGAG
RcaI 410 TCATGA T^CATGA
2547 TCATGA
SfoI 645 GGCGCC GGC^GCC
2782 GGCGCC
TfiI 1187 GAATC G^A[AT]TC
3324 GAATC
XhoI 766 CTCGAG C^TCGAG
2903 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XhoII XmaCI XmaI